2025-05-23
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113542825
External Id
Institute of Oncology research (IOR)
INSDC center name
2023-05-23T15:23:30Z
INSDC last update
public
INSDC status
ERS15539242
SRA accession
E-MTAB-12991:VCaP_Ctrl_rep1
Submitter Id
ArrayExpress
broker name
VCaP
cell_line
prostate gland cancer cell
cell_type
human
common name
Protocols: Cells were plated in 6-well tissue culture plates at 50% confluence. After 24 h, cells were treated with DMSO or 100 nM PRT2527 for 24 h. Cells were lysed with 500 µl TRIzol Reagent (15596026, Thermo Fisher Scientific) RNA was extracted using Direct-zol RNA MiniPrep kit (R2052, Zymo Research). NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was employed with the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) and NEBNext® Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis with the addition of barcode sequences.
description
prostate cancer
disease
wild type genotype
genotype
Homo sapiens
organism
Homo sapiens
organism
prostate gland
organism part
Homo sapiens
scientific_name
VCaP_Ctrl_rep1
title