2025-05-24
ENA first public
ERC000011
ENA-CHECKLIST
École Polytechnique Fédérale de Lausanne (EPFL)
INSDC center name
public
INSDC status
ERS24548840
SRA accession
E-MTAB-13010_3:C3H10_10X_exp14_enriched
Submitter Id
ArrayExpress
broker name
C3H10T1/2
cell line
mesenchymal stem cell
cell type
not collected
collection date
house mouse
common name
Protocols: Prior to use, cells were washed with PBS, dissociated with Trypsin-EDTA (0.05% for HEK293T and 0.25% for C3H10T1/2), resuspended with basic culture medium, filtered using 40 µm strainers and counted with Trypan blue live-dead stain using a Countess cell counter. Both HEK293T and C3H10T1/2 cells were maintained in basic culture medium (containing high-glucose DMEM with GlutaMax and pyruvate, 10% FBS and 1x Penicillin-Streptomycin). All cells were placed at 37 °C and 5% CO2 in a humidified incubator. Using Chromium Single Cell Expression 3’ Reagent Kits Using Chromium Single Cell Expression 3’ Reagent Kits
description
embryo stage
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Mus musculus
scientific_name
C3H
strain
C3H10_10X_exp14_enriched
title