2025-05-24
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland Molecular and Integrative Bioscience Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland
INSDC center name
public
INSDC status
ERS24434214
SRA accession
E-MTAB-15115:A006
Submitter Id
5
month
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: ISCs from 6 PBS and 6 dm-aKG treated mice were sorted  total DNA was isolated using Quick-DNA microprep kit(Zymo). DNA was fractionated using Bioruptor NGS(Diagenode)  after adding control DNAs from the NEBNext Enzymatic 5hmC-seq Kit(NEB-E3350S) to 250 ng of sample. The sonicated DNA libraries were constructed using NEBNext Primers for Epigenetics(Dual Index Primer Pairs Set 2B)(NEB-E3392S).
description
adult
developmental stage
Lgr5-EGFP-IRES-CreERT2(+/-)
genotype
not collected
geographic location (country and/or sea)
PK6341A
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
small intestine
organism part
Mus musculus
scientific_name
female
sex
dm-aKG
stimulus
C57BL/6j
strain
A006
title