2025-05-26
ENA first public
ERC000011
ENA-CHECKLIST
NKI
INSDC center name
public
INSDC status
ERS24690229
SRA accession
CAPH  knockout_Untreated
Submitter Id
2023-06-12
collection date
human
common name
Genomic DNA was isolated from sorted cells using a DNA mini kit (Qiagen) with the lysis step occurring overnight at 56° C for decrosslinking.The gene trap insertion sites were amplified using a LAM-PCR procedure as described (Blomen et al., 2015). Using a biotinylated primer in the gene trap cassette, single-stranded DNA (ssDNA) products were generated for 120 cycles, captured on magnetic beads, a pre-adenylated ssDNA linker ligated to the 3 end, and a final round of exponential amplificationusing a primer containing Illumina sequencing compatible overhangs at the end of the LTR andin the ssDNA linker.
description
Netherlands
geographic location (country and/or sea)
Homo sapiens
organism
Homo sapiens
scientific_name
Synthetic lethality screen in CAPH knockout Untreated
title