0
16S rRNAs recovered
Prokka v1.14.6 (Barrnap v0.9)
16S recovery software
No
16s recovered
0
23S rRNAs recovered
0
5S rRNAs recovered
2025-05-28
ENA first public
ERC000011
ENA-CHECKLIST
d__Bacteria;p__Bacillota_A;c__Clostridia;o__Lachnospirales;f__Lachnospiraceae;g__COE1;s__COE1 sp910585765
GTDB R220 classification
Stanford University School of Medicine
INSDC center name
public
INSDC status
45.819
MAG coverage depth
ERS22948262
SRA accession
METABAT215_SAMESTATE_PRE_SCAFFOLDS_1500_METASPADES__control_F407_F408_F411_F412.50
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics.
assembly quality
metaSPAdes v3.15.4
assembly software
All samples coassembled together were mapped onto the coassembly using bowtie2. Resulting depth information (via jgi_summarize_bam_contig_depths, default settings) was used to bin the coassembly using metaBAT2 (default settings).
binning parameters
metaBAT2 v2.15
binning software
ENVO_01000313
broad-scale environmental context
2021-04-03
collection date
Gradient Boost (General Model)
completeness approach
98.61
completeness score
CheckM2 v1.0.2
completeness software
5.47
contamination score
This sample represents an uncultured Lachnospiraceae bacterium MAG co-assembled and binned from multi-cage mouse samples from several Stanford mice in the control group (resulting in healthy mice).
description
ENVO_00002003
environmental medium
USA
geographic location (country and/or sea)
37.42784
geographic location (latitude)
-122.17000
geographic location (longitude)
metagenome-assembled genome
investigation type
mouse feces
isolation_source
ENVO_2100002
local environmental context
mouse gut metagenome
metagenomic source
not applicable
most complete 16S rRNA recovered
not applicable
most complete 23S rRNA recovered
not applicable
most complete 5S rRNA recovered
32
number of standard tRNAs extracted
uncultured Lachnospiraceae bacterium
organism
Metagenome sequencing of NSG mice with diarrhea
project name
Prokka v1.14.6 (Barrnap v0.9)
rRNA extraction software
No
reassembly post binning
ERS22564061,ERS22564062,ERS22564065,ERS22564066
sample derived from
uncultured Lachnospiraceae bacterium
scientific_name
Illumina NovaSeq 6000
sequencing method
ARAGORN v1.2.41
tRNA extraction software
GTDB-TK v2.4.0, r220 database, default parameters
taxonomic classification
multi-marker approach
taxonomic identity marker
F407_F408_F411_F412__Stanford__control__healthy.50
title
14
unique standard tRNAs extracted