2025-05-29
ENA first public
ERC000011
ENA-CHECKLIST
UOM-BCF
INSDC center name
public
INSDC status
ERS20849854
SRA accession
E-MTAB-14334:Sample 5
Submitter Id
ArrayExpress
broker name
HEK293T
cell_line
immortalized human embryonic kidney cell
cell_type
not collected
collection date
human
common name
Protocols: HEK293T were purchased from ATCC and were grown in DMEM supplemented with 10% FBS. HEK293T cells were co-transfected with plasmids LV-mCherry-T2A-Cas13a and LV-sgRNA-13a-GFP-Neo or LV-sgRNA-13a-GFP-MALAT1 using Polyfect (Qiagen, 301105) following the manufacturer’s protocol. LwaCas13a expression was induced by adding doxycycline (Sigma D9891) to the cells growing in media supplemented with TET-free foetal bovine serum (Takara, 631107) for 48 h. Total RNA isolation was done using HP RNA isolation kit (Roche E3101K) following manufacturer’s instructions. Illumina stranded mRNA library
description
unmodified HEK293T
genetic construct
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
kidney
organism part
Homo sapiens
scientific_name
female
sex
Sample 5
title