2025-05-30
ENA first public
ERC000011
ENA-CHECKLIST
1Mondor Institute for Biomedical Research (IMRB), INSERM, Créteil, France
INSDC center name
public
INSDC status
ERS20529955
SRA accession
E-MTAB-14222:2022_26
Submitter Id
12,3 months
month
age
ArrayExpress
broker name
Tumor MPNST
cell_type
not collected
collection date
house mouse
common name
Protocols: Mice were euthanised via isoflurane inhalation and perfused with PBS to remove circulating immune cells. Tumors were dissected and sampled for (immuno)histological and genomic analyses. Remaining tissue was minced into a slurry and digested in RPMI-1640 medium (Gibco, #42401-018) containing 0,25 mg/ml of Liberase HD (Roche, #05401089001) for 30 min at 37°C. Enzymatic digestion was halted by adding 10% FBS in RPMI (Gibcon #10270106) and tumor cell suspensions were collected by centrifugation (1200 rpmi for 5 min)and mechanical dissociation and filtering (30µm MACS SmartStratiners, Miltenyi Biotec, #130-098-458)was performed to obtain single cell suspension  single-cell suspensions were labelled with DAPI (Invitrogen, #D1306) and subjected to FACS to recover viable cells. 20,000 of viable  were loaded into one channel of the Chromium system using the V3 and V3.1 single-cell reagent kits (10X Genomics Following capture and lysis, cDNAs were synthesized, then amplified by PCR for 12 cycles as per the manufacturer’s protocol (10X Genomics).
description
adult Tumor MPNST
developmental stage
Neurofibromatosis type 1
disease
Prss56cre/+, R26mt/+, NF1flox/-
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Mus musculus
scientific_name
Male
sex
C57BL/6
strain
2022_26
title
xenograft