2025-05-30
ENA first public
ERC000011
ENA-CHECKLIST
MRC Human Genetics Unit, Institute of Genetics and Cancer, University of Edinburgh
INSDC center name
public
INSDC status
ERS20182775
SRA accession
E-MTAB-14144:bl6_he_he_DEN_treated_liver_nodule_15_44044_litterE
Submitter Id
252
day
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Livers from mice were isolated and flash frozen at -80°C. A subgroup of C57BL/6J mice were treated with a single intraperitoneal dose of diethylnitrosamine (20 mg/kg) on post-natal day 15 (P15) to induce liver tumours, which were isolated on day P252. Background liver tissue was also collected.  Samples were treated as previously described in Omni-ATAC protocol (Corces et al. 2017), with minor modifications to the nuclear isolation steps (in Step 1, 1 ml of 1× homogeniser buffer was used instead of 2 ml; in Step 4, douncing was performed with 30 strokes instead of 20). ATAC-seq was performed as described previously (Corces et al. 2017).
description
adult
developmental stage
22/10/15
dob
B6.Ctcf tm1.1Laat/Ctcf+;Speer6-ps1Tg(Alb-cre)21Mgn
genetic modification
CTCF hemizygous
genotype
not collected
geographic location (country and/or sea)
15_44044
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
liver
organism part
15_28660/15_32751
parents (mother/father)
tumour
sampling site
Mus musculus
scientific_name
male
sex
C57BL/6J
strain
bl6_he_he_DEN_treated_liver_nodule_15_44044_litterE
title