2025-05-31
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114497836
External Id
The Racah Institute of Physics The Hebrew University of Jerusalem Jerusalem 9112001, Israel
INSDC center name
2023-10-13T16:00:20Z
INSDC last update
public
INSDC status
ERS16479491
SRA accession
E-MTAB-13428:microcolony_1
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Microcolonies were selected from an anaerobic LB plate are kept at -80C. Bacteria were regrown in LB at 37C with shaking for 5h. DNA was extracted from 1 mL of bacteria using the DNeasy Blood & Tissue Kit (Qiagen). Library preparation and sequencing was carried out by the Genomics Applications Laboratory, Core Research Facility, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel. DNA libraries for sequencing were prepared using the kit (ILMN DNA LP (M) Tagmentation (24 Samples, IPB) 20060060) (Illumina, Inc., USA) according to manufacturing instructions.
description
lrhA mutation
genetic modification
not collected
geographic location (country and/or sea)
not applicable
isolate
microcolony_1
microcolony
Escherichia coli
organism
Escherichia coli
organism
whole organism
organism part
Escherichia coli
scientific_name
O127:H6 strain E2348/69
strain
microcolony_1
title