2025-05-31
ENA first public
ERC000011
ENA-CHECKLIST
Laboratory of Molecular Medicine and Genomics - University of Salerno
INSDC center name
public
INSDC status
siPRAJA
RNA interference
ERS20836968
SRA accession
E-MTAB-14326:E093 siPRAJA input
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: cells were collected using standard protocols  standard protocol standard protocols for cell lines cells where treated with compound for certain number of hours.  standard protocol Total RNA extraction was performed using the TRI reagent (Merck T9424) following the manufacturer's instruction Total RNA concentrations were determined by using NanoDrop spectrophotometer (Thermo Fisher) and their quality were assessed with the TapeStation 4150 (Agilent Technologies). Briefly, 100 ng of RNA of each sample were subjected to mRNAs capture by using polyT-coated beads followed by the indexed library preparation with the SureSelect XT HS2 mRNA Library Preparation System (Agilent Technologies), according to the manufacturer’s instructions
description
glioblastoma
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
E093 siPRAJA input
title