2025-05-31
ENA first public
ERC000011
ENA-CHECKLIST
Universitaetsklinikum Erlangen, Medizin 1
INSDC center name
public
INSDC status
ERS22993331
SRA accession
E-MTAB-14790:O11_Jej_0h
Submitter Id
10
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Mice were sacrificed and its small intestine was collected Total RNA was extracted from the samples taken from colons of mice using the peqGOLD total RNA kit (Peqlab/VWR) following the manufacturers' instructions. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
jejunum
organism part
Mus musculus
scientific_name
male
sex
C57BL/6
strain
O11_Jej_0h
title