2025-05-31
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113577862
External Id
University of Edinburgh Centre for Inflammation Research
INSDC center name
2023-06-07T15:58:36Z
INSDC last update
public
INSDC status
ERS15572776
SRA accession
E-MTAB-13049:FK2L
Submitter Id
16
week
age
ArrayExpress
broker name
not provided
collection date
house mouse
common name
Protocols: Freshly-harvested liver tissues from mice (n = 3 male mice per group) were snap frozen in liquid nitrogen before storage in a low-temperature freezer at -80°C. Total RNA was isolated from the liver tissue samples using the RNeasy Mini Kit (Qiagen) After the QC procedures, mRNA from eukaryotic organisms is enriched using oligo(dT) beads. For prokaryotic organisms or eukaryotic organisms' long-non-coding libraries, rRNA is removed using the Ribo-Zero kit that leaves the mRNA. First, the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.
description
adult
developmental stage
KmoFlpe
genotype
not provided
geographic location (country and/or sea)
2
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
liver
organism part
Mus musculus
scientific_name
male
sex
C57BL6J
strain
FK2L
title