2025-05-31
ENA first public
ERC000011
ENA-CHECKLIST
Novocure
INSDC center name
public
INSDC status
ERS24443167
SRA accession
E-MTAB-15132:Sample 11
Submitter Id
ArrayExpress
broker name
AsPC-1
cell line
not collected
collection date
human
common name
Protocols: Samples were fresh frozen in TRIzol Grown in a 37 °C humidified incubator in RPMI1640 media supplemented with 10% FBS. All media were further supplemented with 2 mM L-glutamine and 50 μg/ml penicillin/streptomycin. TTFields were applied to cells using the inovitroTM system as previously described (Kirson, E.D., et al., Disruption of cancer cell replication by alternating electric fields. Cancer Res, 2004. 64(9): p. 3288-95.) With frequency of 150 kHz and intensity of 1.7 V/cm RMS for 24 and 48 hours.  RNA was purified with TRI reagent as previously described [ Rio, D.C., et al., Purification of RNA Using TRIzol (TRI Reagent). Cold Spring Harbor Protocols, 2010. 2010(6): p. pdb.prot5439. ]   For cDNA libraries preparation, the polyA fraction (mRNA) was purified, followed by fragmentation and generation of double-stranded cDNA. Agencourt AMPure XP beads (Beckman Coulter) were used for cleanup, followed by end repair, UMIs addition, adapter ligation and PCR amplification steps.
description
pancreatic ductal adenocarcinoma
disease
AsPC1
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
pancreas
organism part
3
replicate
Homo sapiens
scientific_name
Baseline
stimulus
Sample 11
title