2025-06-01
ENA first public
ERC000011
ENA-CHECKLIST
Universidad de León
INSDC center name
public
INSDC status
ERS23968679
SRA accession
E-MTAB-15012:Sample 8
Submitter Id
25
day
age
ArrayExpress
broker name
not collected
collection date
sheep
common name
Protocols: Samples from perirenal fat perirenal adipose tissue were collected from each lamb after sacrifice at the commercial slaughterhouse. The tissue was preserved in an RNA-stabilization solution (Ambion RNAlater; Life Technologies) and stored at 4 °C for 24 h. After that, the RNA-stabilization solution was removed, and the samples were later frozen at -80 °C until RNA extraction. The RNA extraction was performed using the miRNeasy Mini KIT (Qiagen) modified to use in adipose tissue. Once the RNA was obtained, the cDNA library construction and sequencing were conducted at Novogene in Cambridge (UK) by using the UltraTM RNA Library Prep Kit (NEBNext®).
description
suckling lamb
developmental stage
protein restriction
diet
wild type genotype
genotype
not collected
geographic location (country and/or sea)
mNC_lf_4
individual
not applicable
isolate
Ovis aries
organism
Ovis aries
organism
perirenal adipose tissue
organism part
Low perirenal fat
phenotype
Ovis aries
scientific_name
male
sex
Assaf
strain
Sample 8
title