2025-06-01
ENA first public
ERC000011
ENA-CHECKLIST
UBT
INSDC center name
public
INSDC status
ERS23861288
SRA accession
319_2022
Submitter Id
temperate biome [ENVO_01001831]
broad.scale.environmental.context
2022-08-10
collection_date
16S rRNA V4 reads from soil taken from an agricultural field near Ruhstorf, Germany. This sample was subjected to natural precipitation.
description
368.17 m
elevation
silty loam [ENVO_06105275]
environmental.medium
sewage sludge (dry), 15000 kg/ha + ALZON (SKW Stickstoffwerke Piesteritz GmbH, Lutherstadt Wittenberg, Germany), 80 kg/ha
fertilizer.regimen
Germany
geographic location (country and/or sea)
48,38241993
geographic.location..latitude.
13,26346437
geographic.location..longitude.
Bavaria, Lower Bavaria, Pocking
geographic.location..region.and.locality.
Laudis Aspekt Pack (Bayer CropScience Deutschland GmbH, Monheim, Germany)
herbicide.regimen
not applicable
host.age
not applicable
host.common.name
not applicable
host.life.stage
not applicable
host.taxid
maize field [ENVO_01000348]
local.environmental.context
Extraction with water instead of sample. Treated like the other samples in the subsequent steps.
negative.control.type
soil metagenome
organism
initial denaturation:98degC_120s; denaturation:98degC_10s; annealing:55degC_30s; extension:72degC_30s; final extension:72degC_120s
pcr.conditions
515f: 5'-GTGYCAGCMGCCGCGGTAA-3', 806RB: 5'-GGACTACNVGGGTWTCTAAT-3'
pcr.primers
not applicable
plant.body.site
Bacterial mock community of the following taxa: Bacillus subtilis (NCBI:txid1423), Chryseobacterium luteum, Clostridium roseum (NCBI:txid421531), Enterobacter mori (NCBI:txid539813), Franconibacter helveticus (NCBI:txid357240), Glycomyces tenuis (NCBI:txid58116), Pseudomonas fluorescens (NCBI:txid294), Pseudomonas putida (NCBI:txid303), Rhizobium endophyticum (NCBI:txid578290), Streptomyces acidiscabies (NCBI:txid42234), Variovorax guangxiensis (NCBI:txid1775474). Amplicons were generated individually for each taxon with the same conditions as for the other samples and pooled in equimolar manner after amplification.
positive.control.type
Rhizo4Bio: RhizoTraits
project.name
natural precipitation
rainfall.regimen
-80 degC
sample.storage.temperature
soil metagenome
scientific_name
none
sequence.quality.check
Illumina iSeq 100
sequencing.method
not applicable
subspecific.genetic.lineage
16S rRNA
target.gene
V4
target.subfragment
prokaryotic microbiome in soil from a maize field
title