2025-06-01
ENA first public
ERC000011
ENA-CHECKLIST
Universitätsklinikum Erlangen Division of Molecular and Experimental Surgery
INSDC center name
public
INSDC status
ERS20525894
SRA accession
E-MTAB-14227:ER30
Submitter Id
ArrayExpress
broker name
primary cell line
cell_line
Cancer associated fibroblast
cell_type
not collected
collection date
human
common name
Protocols: 3 human CAF lines from human CRC and one matching NAFs from uninvolved healthy colon were isolated as described in Naschberger et al, JoVE2018 and Schellerer et al, Oncol Rep 2014 and propagated in culture for up to three passages prior to scRNA-sequencing. Fibroblasts were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich, #S0615) 1% L-Glutamin (Thermo Fisher, #25030-024) and 1% Penicillin/Streptomycin (Gibco, #15140-122) at 37°C, with 8.5% CO2 and 95% humidity. Cultivated fibroblasts were trypsinized, counted and loaded onto a 10x Chromium Next GEM Chip following the Chromium Next GEM Single Cell 3´user guide (10x Genomics). Amplified cDNA was used for 3’ RNA-seq library generation, RNA-seq libraries were prepared following the manufacturer’s user guide (10x Genomics).
description
adult
developmental stage
colorectal cancer
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
colorectum
organism part
Homo sapiens
scientific_name
male
sex
ER30
title