2025-06-01
ENA first public
ERC000011
ENA-CHECKLIST
Department of Systems Biology and Engineering; Silesian University of Technology
INSDC center name
public
INSDC status
ERS21104522
SRA accession
E-MTAB-14492:ST486_IR_12h
Submitter Id
ArrayExpress
broker name
ST486
cell_line
Burkitts lymphoma
cell_type
not collected
collection date
human
common name
Protocols: Burkitt lymphoma and Hodgkin lymphoma cell lines were exposed to ionizing radiation at a dose of 4 Gy and collected 1h, 4h and 12h upon irradiation, control unirradiated cells were collected at 4h timepoint.  Total RNA was extracted using the miRNeasy Mini Kit  (QIAGEN, Cat. No. 1038703). This kit is specifically designed for the isolation of high-quality RNA, including small RNAs such as miRNA, from a variety of sample types. After addition of chloroform, Heavy 5PRIME Phase Lock GelTM (QuantaBio, Cat. No. 2302830) was applied to improve organic/inorganic phase separation. Quality control of RNA was performed using Bioanalyzer. A gel-free, bead-based size selection procedure was applied to isolate RNA fragments ranging between 15 and 40 nucleotides. Libraries were generated with Illumina QIAseq miRNA Library Kit and integrated UMI technology was applied.
description
unspecified
developmental stage
Burkitts lymphoma
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
ascites fluid
organism part
Homo sapiens
scientific_name
Irradiated
stimulus
ST486_IR_12h
title