2025-06-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114567817
External Id
The Racah Institute of Physics The Hebrew University of Jerusalem Jerusalem 9112001, Israel
INSDC center name
2023-11-01T13:11:29Z
INSDC last update
public
INSDC status
ERS16602362
SRA accession
E-MTAB-13514:Sample 1
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Single colonies of wild type EPEC (E2348/69) and CsrA-3XFLAG (NN6861) strains were inoculated from a fresh LB plate in liquid LB and grown over-night without shaking at 37°C. Bacteria were then diluted (1:50) in 200ml of pre-wormed DMEM and grown to OD600 0.4. The bacteria were harvested and subjected to RIL-seq experiments as described (Melamed et al., 2018; Melamed et al., 2016) with modifications to several steps in the protocols RNA was extracted using TRI Reagent (Sigma) as described (Faigenbaum-Romm et al., 2020) Libraries according to (Shishkin et al., 2015) were constructed with several modifications (Melamed et al., 2018).
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Escherichia coli
organism
Escherichia coli
organism
Escherichia coli
scientific_name
EPEC csrA-flagx3_rep1
strain
Sample 1
title