2025-06-01
ENA first public
ERC000011
ENA-CHECKLIST
Department of Systems Biology and Engineering; Silesian University of Technology
INSDC center name
public
INSDC status
ERS21104539
SRA accession
E-MTAB-14491:L428_IR_4h
Submitter Id
ArrayExpress
broker name
L428
cell_line
Hodgkins lymphoma
cell_type
not collected
collection date
human
common name
Protocols: Burkitt lymphoma and Hodgkin lymphoma cell lines were exposed to ionizing radiation at a dose of 4 Gy and collected 4h and 12h upon irradiation, control unirradiated cells were collected at 4h timepoint.  Total RNA was extracted using the miRNeasy Mini Kit  (QIAGEN, Cat. No. 1038703). This kit is specifically designed for the isolation of high-quality RNA, including small RNAs such as miRNA, from a variety of sample types. After addition of chloroform, Heavy 5PRIME Phase Lock GelTM (QuantaBio, Cat. No. 2302830) was applied to improve organic/inorganic phase separation. Upon isolation, RNA was treated with and RNase-Free DNase Set (Qiagen, Cat. No.  79254), to remove possibly remaining DNA.  Libraries were generated with Illumina TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Gold. Standard fragmentation conditions were used (90°C for 2 minutes to obtain 150 nt sequences (modified from standard 94°C for 4 minutes).
description
Adult
developmental stage
Hodgkins lymphoma
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
pleural effusion
organism part
Homo sapiens
scientific_name
Irradiated
stimulus
L428_IR_4h
title