2025-06-01
ENA first public
ERC000011
ENA-CHECKLIST
IBMP-CNRS, Université de Strasbourg
INSDC center name
public
INSDC status
ERS20128874
SRA accession
E-MTAB-14125:f17-1 e2fc-1_2
Submitter Id
10
day
age
ArrayExpress
broker name
not collected
collection date
thale cress
common name
Columbia-0
cultivar
Protocols: Whole 10 day-old in vitro-grown seedlings are snap-frozen in liquid nitrogen before RNA extraction. Arabidopsis sterile seeds are plated on MES-buffered Murashige and Skoog) medium (1X MS salts; Duchefa; 0.8% agar, Fluka; 1% (w/v) sucrose; pH adjusted to 5.7 with KOH). Plated seeds are stratified at 4°C for 2-3 days and then transferred to a plant growth chamber under a 16-h-light/8-h-dark cycle (22/20°C) until collection.  Total RNAs were extracted from 10 day-old whole seedlings in vitro-grown by using Trizol solution (Invitrogen) completed by a second phenol/chloroform treatment. Three biological replicates were used as starting material. RNAs concentrations were determined with a QuBit Fluorometer (Thermo Fisher Scientific). RNAs integrities were checked using the 2100 Bioanalyzer (Agilent).  mRNAs were isolated from total RNAs by using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB) for mRNA libraries preparation. Sequencing libraries were prepared using the Collibri stranded RNA library kit for Illumina (Invitrogen).
description
small rosette (10 day-old)
developmental stage
fbl17-1 (GK_170-E07) e2fc-1 (GK_718E12-025281)
genotype
not collected
geographic location (country and/or sea)
in vitro
growth condition
not applicable
isolate
Arabidopsis thaliana
organism
Arabidopsis thaliana
organism
whole plant
organism part
Arabidopsis thaliana
scientific_name
f17-1 e2fc-1_2
title