2025-06-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114567815
External Id
The Racah Institute of Physics The Hebrew University of Jerusalem Jerusalem 9112001, Israel
INSDC center name
2023-11-01T13:10:02Z
INSDC last update
public
INSDC status
ERS16602358
SRA accession
E-MTAB-13513:Sample 8
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: EPEC ΔgadXYW and EPEC ΔgadXYW, ΔcsrA strains were transformed with pJV300 (NE9069 and NE9090) or pZE12-gadY (NE9070 and NE9091) plasmids. Single colonies of the transformants were grown overnight in LB at 37°C without shaking. Cultures were diluted 100-fold in fresh DMEM, re-grown without shaking at 37°C at 5% CO2 to exponential phase (OD600 = 0.2) and induced with IPTG (1mM, 2h). Cells were centrifuged at 4 °C and resuspended in 50 µl 10 mM Tris-HCl (pH 7.5) containing 1 mM EDTA. Lysosyme was added to 0.5 mg/ml, and samples were subjected to three freeze-thaw cycles. RNA was extracted using TRI Reagent (Sigma) as described (Faigenbaum-Romm et al., 2020) Total expression libraries according to (Shishkin et al., 2015) were constructed with several modifications (Melamed et al., 2018)
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Escherichia coli
organism
Escherichia coli
organism
Escherichia coli
scientific_name
EPEC ΔgadXYW, ΔcsrA pgadY_1
strain
Sample 8
title