2025-06-02
ENA first public
ERC000011
ENA-CHECKLIST
School of Life Sciences, University of Sussex
INSDC center name
public
INSDC status
ERS22557232
SRA accession
E-MTAB-14675:HEL_DMSO_3
Submitter Id
ArrayExpress
broker name
HEL
cell_line
blood cell
cell_type
not collected
collection date
human
common name
Protocols: Myeloid cell lines (K562 and HEL) were treated with DMSO or CHIR99021 (CHIR) overnight. Subsequently, beta-catenin RIP was performed in treated cells. CHIR99021 was used to provide beta-catenin stabilisation, which leads to Wnt/beta-catenin signalling activation. DMSO was used as a vehicle control.  Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (17-700; Sigma-Aldrich) TruSeq Stranded Total RNA libraries made from RNA purified using a RIP protocol
description
adult
developmental stage
acute myeloid leukemia
disease
basal
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
bone marrow
organism part
Homo sapiens
scientific_name
HEL_DMSO_3
title