2025-06-05
ENA first public
ERC000024
ENA-CHECKLIST
Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research
INSDC center name
public
INSDC status
ERS20535316
SRA accession
151160:151170e6-d77e-4465-b30c-13b1549c7c0c
Submitter Id
brackish water [ENVO:00002019]
broad-scale environmental context
GFBIO
broker name
2023-04-23
collection date
sequences were generated in mixed orientation, separate read files submitted for each orientation (here: R1 file contains forward reads)
comment
11.5
m
depth
bottom water sample artifical reef Nienhagen
description
sea water [ENVO:00002149]
environmental medium
water
environmental package
yes
environmental_sample
NF02_lib1
experiment_id
Germany
geographic location (country and/or sea)
54.174985
DD
geographic location (latitude)
11.943486
DD
geographic location (longitude)
artifical reef Nienhagen
geographic location (region and locality)
mimarks-survey
investigation type
artificial reef [ENVO:00000149]
local environmental context
AllPrep DNA/RNA Mini Kit, Cat. No. / ID: 80204
nucleic acid extraction
seawater metagenome
organism
FWD:CCTAYGGGRBGCASCAG REV:GGACTACNNGGGTATCTAAT
pcr primers
Baltic Halichondria microbiome
project name
11.35
psu
salinity
filtration, 0.22 micro m pore size, filters frozen at -80 degrees C
sample material processing
bottom water sample artifical reef Nienhagen
sample_description
seawater metagenome
scientific_name
Illumina MiSeq
sequencing method
true
submitted to insdc
V3-V4
target subfragment
7
temp
NF02
title
11.5
m
total depth of water column
water
water environmental package