2025-06-06
ENA first public
ERC000011
ENA-CHECKLIST
Laboratory of Translational Genomics, Division of Cancer Epidemiology and Genetics, National Cancer Institute
INSDC center name
public
INSDC status
ERS24142035
SRA accession
E-MTAB-15079:C140_REP2
Submitter Id
ArrayExpress
broker name
normal cell line
cell line
melanocyte
cell type
not collected
collection date
human
common name
Protocols: Early passages of primary human melanocytes were isolated from foreskin healthy newborn males, following an established protocol (Halaban et al, J Exp Med (2000) 191(6):1005-1016) from the Specialized Programs of Research Excellence (SPORE) in Skin Cancer Specimen Resource Core at Yale University. Cells were grown in Dermal Cell Basal Medium (American Type Culture Collection/ATCC PCS-200-030) supplemented with Melanocyte Growth Kit (ATCC PCS-200-041) and 1% amphotericin B/penicillin/streptomycin (120-096-711, Quality Biological). C56, C140 and C205 are of European ancestry(CEU) and C24 and C27 are of African descent(YRI). For each library, 50,000 live cells were harvested. The initial Hi-C libraries were generated using the Arima HiC kit (Arima Genomics) following the manufacturer's protocol. Briefly, 2-4 million cells were crosslinked, enzyme digested, and ligated. The ligated DNA were purified by Ampure beads. The ligated DNA was reverse-crosslinked, fragmented by sonication, and size-selected (200-600bp) for adaptor ligation and library amplification using KAPA HyperPrep kit (KAPA Biosystems) following the manufacturer's protocol.The HiC library was then hybridized with the custom capture baits and captured by the SureSelect XT HS and XT low input library preparation kit for ILM (Agilent).
description
neonate
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
C140
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
skin of prepuce of penis
organism part
Homo sapiens
scientific_name
C140_REP2
title