2025-06-06
ENA first public
ERC000011
ENA-CHECKLIST
Vanderbilt University
INSDC center name
public
INSDC status
ERS24089593
SRA accession
E-MTAB-15075:SynNotch hESCs (-)GFP (2)
Submitter Id
ArrayExpress
broker name
embryonic stem cell
cell type
not collected
collection date
human
common name
SynNotch (-)GFP
culture condition
Protocols: Cells were lifted using Accutase and spun down at 300xg for 5 minutes before being resuspended in PBS (w/o calcium or magnesium). H9 hESCs were plated at a density of 400,000 cells/cm2 in 96-well plates coated with Geltrex. SynNotch conditions were seeded at 1:1 ratio of senders:receivers. Neural induction medium was prepared on day 0 and contained neurobasal medium (Gibco, 21103049) supplemented with 1% N2 supplement (Gibco, 17502048), 2% B-27 supplement without vitamin A (Gibco, 12587010), 1% GlutaMAX (Gibco, 35050061), 10 μM SB431542 (STEMCELL TECH, 72234), 100 nM LDN193189 (STEMCELL TECH, 72149), and 10 μM Y-27632. WT H9 hESCs were also plated with 200 ng/mL SHH protein (Sonic C25II, R&D Systems, 464-SH). Medium was prepared (excluding Y-27632) and changed daily, and cells were taken out to 11 days in culture. Cells were encapsulated using the PIPseq method. The PIPseq manufacturer's guide was then followed for nucleic acid extraction. The PIPseq T2 kit v3 chemistry was used. The PIPseq manufacturer's guide was then followed for library preparation.
description
embryo
developmental stage
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
inner cell mass
organism part
Homo sapiens
scientific_name
SynNotch hESCs (-)GFP (2)
title