2025-06-06
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115057672
External Id
Sorbonne-universite INSERM
INSDC center name
2023-12-22T13:13:50Z
INSDC last update
public
INSDC status
ERS17598168
SRA accession
E-MTAB-13670:FIP_Nin+Cel_7
Submitter Id
12
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Mouse epidydimal adipose tissue was harvested and digested in DMEM and collagenase A. After homogenization, the suspension is washed in PBS buffer containing bovine serum albumin (1%) and EDTA (0.5%). After centrifugation, the adipocyte fraction is discarded and the stroma vascular fraction passed through a 100 µm filter before staining.Cell suspensions were stained with appropriate antibodies for 30 min on ice.Cell sorting was performed on a BD FACSAria II™ cell sorter. RNAs from sorted cells were prepared using the RNeasy Plus micro kit (Qiagen). cDNA libraries were generated using the KAPA mRNA HyperPrep kit (Roche).
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
FIP_Nin+Cel_7
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
visceral abdominal adipose tissue
organism part
Mus musculus
scientific_name
male
sex
C3H
strain
FIP_Nin+Cel_7
title
yes
treatment