2025-06-06
ENA first public
ERC000011
ENA-CHECKLIST
IFOM ETS - The AIRC Institute of Molecular Oncology, Milan, Italy
INSDC center name
public
INSDC status
ERS22719672
SRA accession
E-MTAB-14730:HELA-SCR-2
Submitter Id
ArrayExpress
broker name
HeLa
cell_line
epithelial
cell_type
not collected
collection date
human
common name
Protocols: HeLa GFP cells were generated by infecting HeLa WT cells with the pLenti-CMV-MCS-GFP-SV-puro plasmid (#73582, Addgene), previously depleted of the promoter and enhancer regions to generate a stable cell line that does not express the GFP protein. HeLa GFP cells were grown under standard tissue culture conditions (37°C, 5% CO2) in DMEM, supplemented with 10% FBS, 2mM L-Glutamine and 1% Penicillin/Streptomycin. Maxwell® RSC simplyRNA Tissue Kit (Promega) was used to isolate total RNA from cells, according to the manufacturer’s instructions. DNase I (Qiagen) treatment was performed to remove any potential residual contamination of genomic DNA.  Illumina stranded mRNA prep Ligation kit for polyA transcripts
description
adult
developmental stage
adenocarcinoma
disease
scramble
genetic modification
somatic genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
cervix
organism part
Homo sapiens
scientific_name
HELA-SCR-2
title