2025-06-06
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115057309
External Id
UCL GOS Institute of Child Health, University College London
INSDC center name
2023-12-22T11:41:25Z
INSDC last update
public
INSDC status
ERS17597805
SRA accession
E-MTAB-13662:Sample 17
Submitter Id
11
week
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Human embryonic and fetal tissue samples used for bulk-RNA seq were obtained with ethical approval and informed consent from the Human Developmental Biology Resource (http://www.hdbr.org).The age of embryos up to 8wpc was calculated based on Carnegie staging, whereas in older fetuses the age was estimated from foot length and knee-heel length in relation to standard growth data. Samples were karyotyped by G-banding or quantitative PCR (chromosomes 13, 16, 18, 21, 22 and X and Y) to determine the sex of the embryo/fetus and to exclude any major chromosomal rearrangements or aneuploidies. Total RNA was extracted from human fetal brain cortex samples using the AllPrep DNA/RNA Mini Kit (Qiagen) cDNA libraries were prepared using the KAPA mRNA HyperPrep Kit (Roche)
description
11wpc
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
fetal brain cortex
organism part
Homo sapiens
scientific_name
46,XY
sex
Sample 17
title