2025-06-06
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115131601
External Id
UOM-BCF
INSDC center name
2024-01-24T12:10:25Z
INSDC last update
public
INSDC status
ERS17835884
SRA accession
E-MTAB-13735:PNUTS_wtWA-3
Submitter Id
14 to 15
day
age
ArrayExpress
broker name
not collected
collection date
fruit fly
common name
Protocols: Ovaries were dissected in 1 x PBS and then transferred into TRIzol reagent. Flies were grown at 25°C on standard media containing maize, sugar, yeast, agar and anti-fungal agents. RNA was extracted from 10 pairs of ovary/sample. Total RNA was extracted according to the manufacturer’s instructions and RNA was stored in RNAse free water. Quantity and quality of RNA was quantified using Nanodrop ND-2000 (Thermo Fisher Scientific) and Agilent TapeStation system (Agilent). RNA-sequencing libraries were constructed using the Stranded Total RNA Prep. Ligation with Ribo-Zero Plus kit (Illumina, Inc.) according to the manufacturer’s protocol, except with a hybridization step using customised oligonucleotide probes for depletion of Drosophila rRNA.
description
adult
developmental stage
mutant
genotype
not collected
geographic location (country and/or sea)
Pool 12
individual
not applicable
isolate
Drosophila melanogaster
organism
Drosophila melanogaster
organism
ovary
organism part
Drosophila melanogaster
scientific_name
female
sex
w1118; PNUTS13B/ PNUTS13B; PNUTSwt-W762A (InDisruPT)/ovo-FLP
strain
PNUTS_wtWA-3
title