2025-06-06
ENA first public
ERC000011
ENA-CHECKLIST
IFOM ETS - The AIRC Institute of Molecular Oncology, Milan, Italy
INSDC center name
public
INSDC status
ERS22719671
SRA accession
E-MTAB-14730:HELA-G5-3
Submitter Id
ArrayExpress
broker name
HeLa
cell_line
epithelial
cell_type
not collected
collection date
human
common name
Protocols: HeLa GFP cells were generated by infecting HeLa WT cells with the pLenti-CMV-MCS-GFP-SV-puro plasmid (#73582, Addgene), previously depleted of the promoter and enhancer regions to generate a stable cell line that does not express the GFP protein. HeLa GFP cells were grown under standard tissue culture conditions (37°C, 5% CO2) in DMEM, supplemented with 10% FBS, 2mM L-Glutamine and 1% Penicillin/Streptomycin. Maxwell® RSC simplyRNA Tissue Kit (Promega) was used to isolate total RNA from cells, according to the manufacturer’s instructions. DNase I (Qiagen) treatment was performed to remove any potential residual contamination of genomic DNA.  Illumina stranded mRNA prep Ligation kit for polyA transcripts
description
adult
developmental stage
adenocarcinoma
disease
pLenti-CMV-MCS-GFP-SV-puro
genetic modification
somatic genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
cervix
organism part
Homo sapiens
scientific_name
HELA-G5-3
title