2025-06-06
ENA first public
ERC000011
ENA-CHECKLIST
SJTU
INSDC center name
public
INSDC status
ERS23956693
SRA accession
E-MTAB-14997:Panc1-H3Q5A-H3Q5Ser
Submitter Id
ArrayExpress
broker name
pancreatic ductal cell
cell type
not collected
collection date
human
common name
Protocols: The PANC-1 cells were maintained on DMEM supplemented with 10% FBS, 1% penicillin-streptomycin.Collect cells by digesting with trypsin The cells were fixed with 1% formaldehyde and sonicated for 5 minutes on 4 ℃. Next, chromatin fragments were immunoprecipitated with Protein A/G Magnetic beads coupled with antibodies. The eluates were digested with  RNase A and proteinase K. DNA fragments were recovered using the QIAquick PCR purification kit and used for ChIP-seq library construction. ChIP-seq libraries were constructed using NEBNext Ultra II DNA Library Prep kit
description
H3.3Q5A
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
Panc1-H3Q5A-H3Q5Ser
title