2025-06-06
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115057041
External Id
Barts Cancer Institute Queen Mary University of London
INSDC center name
2023-12-22T10:29:47Z
INSDC last update
public
INSDC status
ERS17597537
SRA accession
E-MTAB-13655:CRC11_LM
Submitter Id
30-85
year
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Metastatic colorectal cancer tissue was provided by the Barts Cancer Tissue Bank. We accessed archived frozen tissue samples and collected fresh tissue samples from the Royal London Hospital. Fresh tissue samples were flash frozen in a dry ice/ethanol bath and stored at -80°C. 10 µm sections were taken from fresh frozen liver metastatic CRC samples from three patients. The tissue permeabilisation time was optimised using a Visium Spatial Tissue Optimization Slide & Reagent Kit (10x Genomics, 1000193), following the manufacturer’s instructions (Rev D). A 6 minute permeabilisation time was selected. Spatial transcriptomic libraries were created using a Visium Spatial Gene Expression Slide & Reagent Kit (10x Genomics, 1000187), following the manufacturer’s instructions (Rev E).
description
adult
developmental stage
colorectal cancer
disease
not collected
geographic location (country and/or sea)
CRC11
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
liver
organism part
colorectal cancer liver metastasis
sampling site
Homo sapiens
scientific_name
CRC11_LM
title