2025-06-06
ENA first public
ERC000011
ENA-CHECKLIST
Laboratory of Translational Genomics, Division of Cancer Epidemiology and Genetics, National Cancer Institute
INSDC center name
public
INSDC status
ERS24001610
SRA accession
E-MTAB-15048:MELANOCYTE_C24_rep1
Submitter Id
ArrayExpress
broker name
normal cell line
cell line
melanocyte
cell type
not collected
collection date
human
common name
Protocols: Early passages of primary human melanocytes were isolated from foreskin healthy newborn males, following an established protocol (Halaban et al, J Exp Med (2000) 191(6):1005-1016) from the Specialized Programs of Research Excellence (SPORE) in Skin Cancer Specimen Resource Core at Yale University. Cells were grown in Dermal Cell Basal Medium (American Type Culture Collection/ATCC PCS-200-030) supplemented with Melanocyte Growth Kit (ATCC PCS-200-041) and 1% amphotericin B/penicillin/streptomycin (120-096-711, Quality Biological). C56, C140 and C205 are of European ancestry(CEU) and C24 and C27 are of African descent(YRI). For each library, 50,000 live cells were harvested. The ATAC-seq libraries were generated following a previously published protocol (Buenrostro et al., Nature Methods, 2013). The nuclei were isolated from the 50,000 cells. The isolated nuclei were treated with Tn5 Transposes from Nextera DNA Sample Preparation Kit and the transposed DNA was purified using a Qiagen MinElute Kit. The transposed DNA libraries were amplified by PCR for 12 cycles and isolated by Ampure XP beads.
description
neonate
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
C24
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
skin of prepuce of penis
organism part
Homo sapiens
scientific_name
MELANOCYTE_C24_rep1
title