2025-06-10
ENA first public
ERC000011
ENA-CHECKLIST
Istituto Nazionale di Genetica Molecolare (INGM)
INSDC center name
public
INSDC status
ERS20620903
SRA accession
E-MTAB-14299:Sample 5
Submitter Id
ArrayExpress
broker name
MEF cell line
cell_line
mouse embryonic fibroblast cell
cell_type
not collected
collection date
house mouse
common name
Protocols: Immortalized MEF cells were washed once with ice-cold PBS 10ug/ml cyclohexamide.  Cells were lysed in 20mM Tris-HCl, 50mM NaCl, 10mM MgCl2, 1mM DTT, 100ug/ml cyclohexamide, 1% NP-40, 0.2mg/ml heparin. Ribosome Protected Fragments (RPF) were obtained following Ingolia 2017 (10.1016/j.ymeth.2017.05.028).  Immagina Biotechnology LACEseq #LS-001_12
description
Eif6 S235A
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
embryonic tissue
organism part
Mus musculus
scientific_name
Sample 5
title