2025-06-11
ENA first public
ERC000024
ENA-CHECKLIST
Ifremer
INSDC center name
public
INSDC status
Production of targeted PCR from 10 ng of DNA material. The aim is to generate amplified targeted regions indexed with secondary identifiers or BID = Barcoded Identifiers. In the protocol, the region of interest is amplified using PCR Forward and Reverse primers flanked by sequences of 8 bases, called "BID" (the name has been developed at Genoscope to differentiate from other MIDs = Molecular Identifiers already in use at the time). To date, 18 different BID sequences have been tested, 12 of which have been selected. The amplicons generated will be then normalized and pooled by up to 12 (amplicons carrying different BID sequences). Illumina libraries will be prepared by Genoscope on each pool of BIDed amplicons and then Illumina sequenced
LIBRARY_CONSTRUCTION_PROTOCOL
ERS24836890
SRA accession
sam_BIOCEAN5D-zooplankton_NC_BID04_F3_PL3
Submitter Id
neritic epipelagic zone biome
broad-scale environmental context
SeBiMER
broker name
PDG-ODE-DYNECO
collected_by
IFREMER
collecting institution
not applicable
collection date
PDG-ODE-DYNECO
collector name
blank sample
common name
missing: control sample
m
depth
seawater
environmental medium
water
environmental package
No
environmental_sample
sampling site,time,distance from the coast
experimental factor
not applicable
geographic location (country and/or sea)
not applicable
m
geographic location (elevation)
not applicable
DD
geographic location (latitude)
not applicable
DD
geographic location (longitude)
not applicable
geographic location (region and locality)
metagenome
investigation type
Blank sample
isolation_source
bay
local environmental context
TATGTCAG
multiplex identifiers
blank sample
organism
F: GGWACWGGWTGAACWGTWTAYCCYCC  /  R: TAIACYTCIGGRTGICCRAARAAYCA
pcr primers
ROME
project name
Negative control with barcode identifier "NC_BID04_F3_PL3"
sample_description
NC_BID04_F3_PL3
sample_name
blank sample
scientific_name
COX1
target gene
not applicable
target subfragment
NC_BID04_F3_PL3
title