2025-06-12
ENA first public
ERC000047
ENA-CHECKLIST
EMG
INSDC center name
public
INSDC status
ERS24935361
SRA accession
SRR18550247_metabat2_13
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
metaspades_v3.15.5
assembly software
default
binning parameters
MGnify-genomes-generation-pipeline_v1.0.0
binning software
maquatic
broad-scale environmental context
EBI-EMG
broker name
2017-06
collection date
97.81
%
completeness score
CheckM2_v1.0.1
completeness software
1.31
%
contamination score
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic run SRR18550247 of study SRP278837.
description
biofilter substrate
environmental medium
USA
geographic location (country and/or sea)
missing: third party data
DD
geographic location (latitude)
missing: third party data
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
sediment metagenome
isolation_source
aquarium system
local environmental context
sediment metagenome
metagenomic source
uncultured Gammaproteobacteria bacterium
organism
In commercial large-scale aquaria, microorganisms capable of transforming toxic nitrogen species into more benign species play important roles in system health. Many microbes convert nitrite and ammonium into nitrate via nitrification. However, nitrate is toxic to macrofauna at high concentrations. Some bacteria within the phylum Planctomycetes transform ammonium and nitrite to nitrogen gas via anaerobic ammonium oxidation (anammox). Here, we characterized microbial communities in four habitats at the Tennessee Aquarium and examined the abundance and anammox potential of Planctomycetes using culture-independent approaches. 16S rRNA gene amplicon sequencing of habitats revealed distinct, yet stable, microbial communities and the presence of Planctomycetes (~1-15% of each community). Metagenomic analyses identified the genetic potential for multiple complete nitrogen metabolism pathways. However, no known genes diagnostic of the anammox reaction were found in this survey. Given the low relative abundance of these bacteria, a nested Planctomycete-specific 16S rRNA gene-based PCR approach was used to (i) enrich amplicons representative of group members and (ii) identify anammox bacteria based on diagnostic genetic signatures. This amplicon enrichment identified highly diverse sequences that share <95% 16S rRNA gene sequence identity to cultured Planctomycetes, revealing an opportunity to study novel strains within this phylum. These data indicate that putative anammox organisms are present within these exhibits, but further study is required to confirm their capability to perform the anammox reaction.
project name
SAMN15908822
sample derived from
uncultured Gammaproteobacteria bacterium
scientific_name
Illumina MiSeq
sequencing method
multi-marker approach
taxonomic identity marker
Metagenome-assembled genome: SRR18550247_metabat2_13
title