2025-06-13
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114192174
External Id
(1) Laboratory of Angiogenesis and Vascular Metabolism, Department of Oncology, KU Leuven, Leuven, B-3000, Belgium; (2) Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology, VIB, Leuven, B-3000, Belgium; (3) Department of Molecular Biology and Biochemistry, Faculty of Sciences, Andalucía Tech, University of Má-laga and IBIMA (Biomedical Research Institute of Málaga)-BIONAND Platform, Málaga, 29590, Spain.
INSDC center name
2023-07-24T15:42:48Z
INSDC last update
public
INSDC status
ERS16172867
SRA accession
E-MTAB-13198:DXX_PEX5
Submitter Id
ArrayExpress
broker name
HUVEC
cell_line
not collected
collection date
human
common name
Protocols: Endothelial cells were cultured and RNA of control and PEX5 Knockdown ECs. RNA was extracted using TRIzol (Thermo Fisher Scientific) following the manufacturer’s instructions. RNA quality and quantity were measured on a Nanodrop (Thermo Fischer Scientific). Poly-adenylated fragments were isolated from 1 μg total RNA. These fragments were reverse transcribed and converted into indexed sequencing libraries using the KAPA stranded mRNA-seq kit (Sopachem).
description
embryo
developmental stage
normal
disease
PEX5 knockdown by shRNA
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
umbilical vein endothelium
organism part
Homo sapiens
scientific_name
DXX_PEX5
title