2025-06-13
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114751367
External Id
Dioscuri Centre for Chromatin Biology and Epigenomics, Nencki Institute of Experimental Biology, Polish Academy of Sciences
INSDC center name
2023-11-27T13:50:48Z
INSDC last update
public
INSDC status
ERS17170960
SRA accession
E-MTAB-13572:NS_CTCF-Cterm_HALO_Ddx5-KO_CE10
Submitter Id
ArrayExpress
broker name
embryonic stem cell derived neuronal stem cell
cell_line
neuronal stem cell
cell_type
not collected
collection date
house mouse
common name
Protocols: Cells were detached with Accutase, counted and crosslinked with 1% (final) formaldehyde in their medium. The reaction was quenched with 0.125M (final) glycine, and cell pellets were washed with PBS, snap-frozen, and stored in a -80 freezer. The pellet of 5 million cells was used for each Hi-C experiment. NS cells were grown on laminin-coated plates in neural differentiation medium containing N2 and B27 supplements along with recombinant murine EGF and bFGF to a final concentration of 10 ng/ml. Cells were split at 80% confluence with a density of 35000 cells/sq.cm. The medium was exchanged daily. Hi-C experiments were performed as described as describer before (Rao et al., 2014) Hi-C libraries were generated using NEBNext Ultra II DNA Library Kit, according to manufacturer's instructions
description
embryo
developmental stage
normal
disease
gene knock out
genetic modification
DDX5 KO
genotype
not collected
geographic location (country and/or sea)
Mus musculus
organism
Mus musculus
organism
inner cell mass
organism part
Mus musculus
scientific_name
NS_CTCF-Cterm_HALO_Ddx5-KO_CE10
title