SAMN00150936
External Id
LIBGSS_000046
External Id
University of Texas at El Paso
INSDC center name
1998-07-13T00:00:00Z
INSDC first public
2025-06-09T21:09:57.537Z
INSDC last update
live
INSDC status
Generic
NCBI submission model
Generic.1.0
NCBI submission package
ERS24947506
SRA accession
Vector was digested to produce ends compatible with Sau3a. G. lamblia DNA was partially digested with Sau3A to yield fragments in the 35 Kb range and then was de-phosphorylated. Vector and insert with ligated with DNA ligase, pacakaged into lambda phage with Gigapack Gold (Stratagene), and the E. coli host DH5-alpha was infected with the phage. Individual clones were picked from plates and arrayed into 96-well plates. The name cLM-5a12-t indicates that the clone is found on plate 5 in position a12 and was sequenced with the t3 primer. The suffix -u means the sequence was derived from the t7 end. The clones in the DH5-alpha background were much easier to sequence.
description
trophozoite
http://www.ebi.ac.uk/efo/EFO_0002592
dev-stage
E. coli, DH5-alpha
lab-host
Giardia duodenalis
http://purl.obolibrary.org/obo/NCBITaxon_5741
organism
BamHI/Sau3A
re_1
BamHI/Sau3A
re_2
WB
http://www.ebi.ac.uk/efo/EFO_0002871
strain
Whole organism
http://purl.obolibrary.org/obo/UBERON_0000468
tissue-type
cLM
title
Cosmid
v_type
S-cos-1
vector