2025-06-13
ENA first public
ERC000011
ENA-CHECKLIST
The Research Institute of the McGill University Health Centre
INSDC center name
public
INSDC status
ERS24556380
SRA accession
E-MTAB-15150:Female Unstable VSMCs
Submitter Id
66
year
age
ArrayExpress
broker name
vascular smooth muscle cell
cell type
not collected
collection date
human
common name
Protocols: Carotid atherosclerotic plaque specimens were collected from the operating room and immediately placed in ice cold sterile phosphate-buffered saline (PBS). All samples were processed within 30 minutes of collection. Each specimen was weighed, and a small portion from the area of maximal stenosis was kept for histological analysis to grade atherosclerotic plaque stability using Hematoxylin and Eosin, as previously described10-11. Plaque instability was determined based on two gold-standard classification methods: (i.) the American Heart Association (AHA) classification by Stary et al., and (ii) the semiquantitative scale by Lovett et al. Evaluating pathologists were blinded to the patients’ cerebrovascular symptomatic status, demographic details, and all pertaining clinical information. Single-cell suspensions were prepared and loaded into the 10x Genomics Chromium Controller or Chromium X instrument using the Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1, following the manufacturer’s protocol. Cells were encapsulated into Gel Beads-in-Emulsion (GEMs) containing barcoded oligonucleotides. Within each GEM, cells were lysed, and mRNA was reverse transcribed into barcoded cDNA. Post reverse transcription, GEMs were broken, and cDNA was purified using Dynabeads. The cDNA underwent amplification via PCR, followed by fragmentation, end-repair, A-tailing, and adaptor ligation. Indexed libraries were then amplified and purified using SPRIselect beads. Library quality and quantity were assessed using LabChip GX and qPCR, respectively.
description
unstable carotid atherosclerotic plaque
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
female
sex
Female Unstable VSMCs
title