characteristics.text: 2025-06-14
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: I2BC
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS24804326
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15189:Het_HP1g-Rep2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: bipotential mouse embryonic liver
characteristics.characteristic: cell line
characteristics.text: bipotential mouse embryonic liver
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: house mouse
characteristics.characteristic: common name
characteristics.text: Protocols: BMEL cells were trypsinized and permeabilized with NE1 buffer as per https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v3. BMEL cells were cultured in RPMI 1640 medium (Invitrogen) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, 10 µg/mL insulin, 30 ng/mL IGF-II, and 50 ng/mL EGF, at 37 °C under 5% CO2. Permeabilized cells were treated for Cut & Tag experiments as per https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v3. Libraries were PCR amplified for Illumina sequencing using primers described in https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v3
characteristics.characteristic: description
characteristics.text: mouse embryo stage
characteristics.characteristic: developmental stage
characteristics.text: normal
characteristics.characteristic: disease
characteristics.text: Cbx1+/+,Cbx3+/+,Cbx5+/-
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: Mus musculus
characteristics.characteristic: organism
characteristics.text: liver
characteristics.characteristic: organism part
characteristics.text: Mus musculus
characteristics.characteristic: scientific_name
characteristics.text: Het_HP1g-Rep2
characteristics.characteristic: title