characteristics.text: 2025-06-14
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: Molecular Cell Biology
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS24799512
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15180:Sample 13
characteristics.characteristic: Submitter Id
characteristics.text: adult
characteristics.characteristic: age
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: Protocols: The worms were cultivated on 8P agar plates coated with NA22 E. coli at 20 °C, 750 worms were isolated after treatment for RNA isolation.  RNA was isolated from 750 worms in 1 mL TRI reagent® (Sigma-Aldrich). Isolated RNA was purified with the RNA clean and concentrator™-5 Kit (Zymo Research) according to the manufacturers instructions.  Library preparation was performed with 300 ng RNA using the NEBNext® Ultra™ II directional RNA library prep kit for Ilumina® with 14 PCR cycles according to manufacturers instructions.
characteristics.characteristic: description
characteristics.text: D2
characteristics.characteristic: developmental stage
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: 750 individuals
characteristics.characteristic: individual
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: organism
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: organism
characteristics.text: whole worm
characteristics.characteristic: organism part
characteristics.text: Caenorhabditis elegans
characteristics.characteristic: scientific_name
characteristics.text: hermaphrodite
characteristics.characteristic: sex
characteristics.text: 24hFAC+24hFAC
characteristics.characteristic: stimulus
characteristics.text: Bristol N2
characteristics.characteristic: strain
characteristics.text: Sample 13
characteristics.characteristic: title