characteristics.text: SAMN41738566
characteristics.characteristic: External Id
characteristics.text: United States Environmental Protection Agency
characteristics.characteristic: INSDC center name
characteristics.text: 2025-06-06T00:50:53.867Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-06-06T00:50:53.867Z
characteristics.characteristic: INSDC last update
characteristics.text: SRS21546339
characteristics.characteristic: INSDC secondary accession
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: SRS21546339
characteristics.characteristic: SRA accession
characteristics.text: 5-6 dpf
characteristics.characteristic: age
characteristics.text: US EPA Great Lakes Toxicology & Ecology Division, 6201 Congdon Blvd, Duluth, MN 55804
characteristics.characteristic: biomaterial_provider
characteristics.text: US EPA Great Lakes Toxicology & Ecology Division, 6201 Congdon Blvd, Duluth, MN 55804
characteristics.characteristic: birth_location
characteristics.text: not applicable
characteristics.characteristic: breed
characteristics.text: All larval fathead minnows (Pimephales promelas) used for testing were obtained from an on-site culture facility at the US EPA Great Lakes Toxicology and Ecology Division in Duluth, MN, USA. Fathead minnow embryos were collected from multiple crosses of at least three females in paired breeding. Fertilized embryos that were attached to polyvinyl chloride breeding tiles were transferred to a bath of aerated Lake Superior water and held at 25 °C. All embryos were fertilized within a two-hour window. At five-day post-fertilization, larvae that had hatched and were freely swimming were collected, pooled, and randomly sorted for use. All procedures involving larval fathead minnows were approved by an animal care and use committee in accordance with the Guide for the Care and Use of Laboratory Animals (National Research Council, 2011; US EPA Animal Care and Use Plan # Eco24-09-001).
characteristics.characteristic: breeding_method
characteristics.text: Great Lakes Toxicology & Ecology Division
characteristics.characteristic: collected_by
characteristics.text: 2022-07
characteristics.characteristic: collection_date
characteristics.text: not applicable
characteristics.characteristic: cultivar
characteristics.text: larval
characteristics.characteristic: dev_stage
characteristics.text: not applicable
characteristics.characteristic: ecotype
characteristics.text: 24 h
characteristics.characteristic: exposure_duration
characteristics.text: USA: Minnesota, Duluth
characteristics.characteristic: geo_loc_name
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: 66.67
characteristics.characteristic: nominal_conc
characteristics.text: µM
characteristics.characteristic: nominal_conc_units
characteristics.text: Pimephales promelas
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_90988
characteristics.characteristic: organism
characteristics.text: FHM001
characteristics.characteristic: plate_ID
characteristics.text: D1_A11
characteristics.characteristic: replicate_ID
characteristics.text: Total RNA
characteristics.characteristic: sample_type
characteristics.text: not determined
characteristics.characteristic: sex
characteristics.text: -80 deg C
characteristics.characteristic: store_cond
characteristics.text: GLTED-Duluth-wt
characteristics.characteristic: strain
characteristics.text: whole body
characteristics.characteristic: tissue
characteristics.text: RNA-seq of whole, larval P. promelas after 24 h exposure to Cetirizine
characteristics.characteristic: title
characteristics.text: Cetirizine
characteristics.characteristic: treatment
characteristics.text: Chem9
characteristics.characteristic: treatment_group