characteristics.text: SAMN41392178
characteristics.characteristic: External Id
characteristics.text: Institut Pasteur de Montevideo
characteristics.characteristic: INSDC center name
characteristics.text: 2025-06-01T00:53:14.517Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-06-01T00:53:14.517Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: ERS24970784
characteristics.characteristic: SRA accession
characteristics.text: 8 weeks
characteristics.characteristic: age
characteristics.text: 2023-02-13
characteristics.characteristic: collection_date
characteristics.text: Differentiated Bone Marrow Derived Cells, containing dendritic cells (BMDCs), were cultured from bone marrow cell precursors as described in (Segovia et al. 2019). Briefly, bone marrow cells were collected form 8 weeks-old female C57BL/6 mice and differentiated with 0.4 ng / mL GM-CSF for 8 days in RPMI supplemented with 10 % heat-inactivated FBS, 2mM glutamine, non-essential amino acids, 1 mM sodium pyruvate, 10 mM HEPES, 0.05 mM Beta-mercaptoethanol and 100 U / mL Penicillin-Streptomycin. At day 8, cells were harvested and used as needed.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: dev_stage
characteristics.text: Uruguay: Montevideo
characteristics.characteristic: geo_loc_name
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: female
characteristics.characteristic: sex
characteristics.text: C57BL/6
characteristics.characteristic: strain
characteristics.text: Bone marrow
characteristics.characteristic: tissue
characteristics.text: Murine bone marrow-derived innate immune cells (BMDCs)
characteristics.characteristic: title