characteristics.text: 2025-06-14
characteristics.characteristic: ENA first public
characteristics.text: ERC000011
characteristics.characteristic: ENA-CHECKLIST
characteristics.text: University of Toronto, St. Michael's Hospital
characteristics.characteristic: INSDC center name
characteristics.text: public
characteristics.characteristic: INSDC status
characteristics.text: ERS24799099
characteristics.characteristic: SRA accession
characteristics.text: E-MTAB-15177:Modified drug Flu 2
characteristics.characteristic: Submitter Id
characteristics.text: ArrayExpress
characteristics.characteristic: broker name
characteristics.text: primary cell line
characteristics.characteristic: cell line
characteristics.text: endothelial cell
characteristics.characteristic: cell type
characteristics.text: not collected
characteristics.characteristic: collection date
characteristics.text: human
characteristics.characteristic: common name
characteristics.text: Protocols: Total RNA was isolated from human primary microvascular endothelial cells (HPMECs) seeded on 6 well plates (3.8 x 105cells/well). HPMECs were infected with influenza and treated with DMSO, Ap (1 μM) or Ap-1 (a modified inactive derivative of Ap) for two hours.  Lysates were prepared by washing cells twice with PBS+, lysed in recommended volume of RLT Lysis buffer from the Qiagen RNeasy RNA Isolation Kit (Cat. No. / ID: 74104) and collected by scraping with a sterile cell scraper. Lysates were vortexed for 20 seconds, centrifuged at max speed for 3 minutes at room temperature at stored at -80C for up to two weeks prior to extraction with the kit which was performed according to manufacturer’s instructions.  Library prep was performed using the NEB Ultra II Directional mRNA kit.
characteristics.characteristic: description
characteristics.text: adult
characteristics.characteristic: developmental stage
characteristics.text: influenza A (H1N1)
characteristics.characteristic: disease
characteristics.text: wild type genotype
characteristics.characteristic: genotype
characteristics.text: not collected
characteristics.characteristic: geographic location (country and/or sea)
characteristics.text: not applicable
characteristics.characteristic: isolate
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: Homo sapiens
characteristics.characteristic: organism
characteristics.text: endothelium
characteristics.characteristic: organism part
characteristics.text: Homo sapiens
characteristics.characteristic: scientific_name
characteristics.text: Modified drug Flu 2
characteristics.characteristic: title