characteristics.text: SAMN39588022
characteristics.characteristic: External Id
characteristics.text: Shanghai ninth people's hospital
characteristics.characteristic: INSDC center name
characteristics.text: 2025-06-01T00:53:07.913Z
characteristics.characteristic: INSDC first public
characteristics.text: 2025-06-01T00:53:07.913Z
characteristics.characteristic: INSDC last update
characteristics.text: live
characteristics.characteristic: INSDC status
characteristics.text: Model organism or animal
characteristics.characteristic: NCBI submission model
characteristics.text: Model.organism.animal.1.0
characteristics.characteristic: NCBI submission package
characteristics.text: ERS24969099
characteristics.characteristic: SRA accession
characteristics.text: 4-6 weeks
characteristics.characteristic: age
characteristics.text: 2022-12-12
characteristics.characteristic: collection_date
characteristics.text: Primary BMDMs were obtained from male C57BL/6 miceaged 4-6 weeks. The mice were euthanized, and femurs and tibias were removed. BMDMs were flushed out of the bones using a cold culture medium, filtered through a 70-um cell strainer , and centrifuged at 1500 rpm for 5 min. The cells were suspended in cold culture medium supplemented with 20 ng/ml M-CSF and cultured for five days, with half of the medium being replaced on day three and full replacement on day 5 before being used for subsequent experiments.The culture medium was alpha-MEM supplemented with 10% FBS and 1% penicillin-streptomycin-gentamicin solution. Mature BMDMs were double-stained using flow cytometry antibodies CD16/32 and F4/80 for identification. The recombinant protein AnxA5 used in this study was produced by SEME Cell Technology Co., Ltd, China. To investigate the role of AnxA5 in regulating macrophage polarization in an inflammatory environment, 100000 BMDMs were seeded in 12 well plates. After 24 hours of cell seeding, the regular medium was replaced with culture medium (control group, 1-1, 1-2 and 1-3), culture medium containing 1 ug/mL lipopolysaccharide and 30 ng/mL interferon gama (LPS and IFN gama group, 2-1, 2-2 and 2-3), culture medium containing 1 ug/mL LPS, 30 ng/mL IFN gama, and different 2 ug/mL of AnxA5 (Treat group, 4-1, 4-2 and 4-3) for 6 hours
characteristics.characteristic: description
characteristics.text: China
characteristics.characteristic: geo_loc_name
characteristics.text: bone marrow-derived macrophages (BMDMs)
characteristics.characteristic: isolate
characteristics.text: Mus musculus
characteristics.ontologyTerms: http://purl.obolibrary.org/obo/NCBITaxon_10090
characteristics.characteristic: organism
characteristics.text: male
characteristics.characteristic: sex
characteristics.text: bone marrow
characteristics.characteristic: tissue
characteristics.text: Bone marrow-derived macrophages from Mus musculus
characteristics.characteristic: title