SAMN48925073
External Id
Hubei University
INSDC center name
2025-06-06T00:00:00Z
INSDC first public
2025-06-06T07:01:14.797Z
INSDC last update
SRS25288106
INSDC secondary accession
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
SRS25288106
SRA accession
2024-03-15
collection_date
China: Qingdao
geo_loc_name
Under exogenous lactate stress (40 g/L), an AI-powered Digital Colony Picker (DCP) was employed to autonomously screen and export microbial clones based on integrated growth and metabolic phenotyping. Individual cells were randomly compartmentalized into static picoliter-scale droplets within an arrayed microfluidic chip containing 16,000 microchambers. AI-driven image analysis enabled continuous monitoring of single-cell morphology, proliferation dynamics, and fluorescence-based metabolic activities. A lactate-tolerant mutant strain (designated S-3-4) was subsequently isolated using contact-free laser-induced bubble (LIB) ejection technology.
isolation_source
Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
http://purl.obolibrary.org/obo/NCBITaxon_264203
organism
single cell
sample_type
ZM4
strain
mobilis
sub_species
Microbe sample from Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
title