SAMN49009163
External Id
Massachusetts Institute of Technology
INSDC center name
2025-06-11T00:00:00Z
INSDC first public
2025-06-11T20:10:02.201Z
INSDC last update
SRS25347946
INSDC secondary accession
live
INSDC status
Microbe, viral or environmental
NCBI submission model
Microbe.1.0
NCBI submission package
SRS25347946
SRA accession
2024-04-08
collection_date
Rubisco GWS1B under T7 promoter was selectively mutated in MutaT7-Dual8 E. coli for 24h per round of directed evolution. Two plasmids were present in the starting culture: BAC-GWS1B containing Rubisco and pACYC-prk::nptII to enable Rubisco selection. Both plasmids were purified from all cell material on a selective agar plate and sequenced after each evolution round (excluding 5 and 7). Rescreened plasmid pools were selectively digested, retransformed into E. coli, selected on agar plates, and sequenced again.
description
USA:Cambridge
geo_loc_name
Gallionellaceae
host
Evolution_Round_4
mating_type
Escherichia coli
http://purl.obolibrary.org/obo/NCBITaxon_562
organism
cell culture
sample_type
MutaT7-Dual8
strain
Sequencing of plasmid pools from Round 4 of evolution in MutaT7-RDE
title