SAMN49005398
External Id
Jiangsu University
INSDC center name
2025-06-11T00:00:00Z
INSDC first public
2025-06-11T13:18:29.457Z
INSDC last update
SRS25344830
INSDC secondary accession
live
INSDC status
Metagenome or environmental
NCBI submission model
Metagenome.environmental.1.0
NCBI submission package
SRS25344830
SRA accession
2023
collection_date
For viral metagenomic analysis, cerebrospinal fluid (CSF) samples were collected from 10 patients with encephalitis of unknown etiology in 2023. The 10 CSF samples were randomly pooled into a single specimen pool. This pooled sample was resuspended in 1 mL DPBS and subjected to three rapid freeze-thaw cycles on dry ice. After centrifugation (10 minutes, 15,000 g), the supernatant was collected and filtered through a 0.45 uL membrane (Millipore) to remove eukaryotic and bacterial cell-sized particles. From the filtered supernatant, 200 uL was treated with a nuclease cocktail to reduce free (non-viron-protected) nucleic acids. Total nucleic acids were extracted from the treated sample using the QIAamp MinElute Virus Spin Kit per the manufacturer's protocol. A single library was prepared with the Nextera XT DNA Sample Preparation Kit (Illumina) and sequenced on the MiSeq Illumina platform, generating 250 bases paired ends with dual barcoding for the pool.
description
China:Zhenjiang,Jiangsu
geo_loc_name
Homo sapiens
host
cerebrospinal fluid
isolation_source
missing
lat_lon
human metagenome
http://purl.obolibrary.org/obo/NCBITaxon_646099
organism
Metagenome or environmental sample from Human metagenome
title