1
Amount of dCas9 added (ul)
BAR-CAT 1.0
BAR-CAT Version Used
67649
Barcode_Dropouts
20
Bead resuspension OR Elution vol (ul)
18
Cumulative wash volume (mL)
Supercoiled
DNA Library Format (Supercoiled vs. Linear)
500
DNA input amount (ng)
2
Enriched DNA volume (ul) used per qPCR
15
Enrichment incubaton time at 37 C (min)
1384
Expected Number of Spacers in sgRNA library
SAMN48946957
External Id
0.80935
Fraction of Pop Not in Original Scrape
0.84512
Gini of Barcodes After Enrichment
University of Oregon
INSDC center name
2025-06-07T00:00:00Z
INSDC first public
2025-06-07T14:14:02.400Z
INSDC last update
SRS25298039
INSDC secondary accession
live
INSDC status
pEVBC8-DHFR-S2
Library Plasmid
5
Magnetic beads per enrichment (ul) before dilution
0.92336
Median Fold Enrichment (ignoring dropouts)
-0.11503
Median Log2 Enrichment Score (ignoring dropouts)
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
846480
Number of 20 nt BC reads per Llibrary
93418
Number of Barcodes in DNA Library
113000
Number of Enriched Non-Target Barcodes
520
Number of Enriched Targeted Barcodes (Coverage?)
1536
Number of Genes in DNA Library
1G
Number of Guanines Before Spacer
30
Number of PCR Cycles used to Amplify Enriched DNA
684
Number of Targeted Barcodes
1296
Observed Number of Spacers (Coverage)
0.99701
Off-Targets Population Fraction Enrichment Score (all)
SRS25298039
SRA accession
548
Target_Dropouts
1.24154
Targets Population Fraction Enrichment Score
9
Total number of washes
859878
Total reads/sequencing depth?
2
Wash volume per wash (mL)
not applicable
age
not applicable
collection_date
1
dCas9 Concentration (uM)
Proteinase K
dCas9 Denaturation
1.11
dCas9 to sgRNA ratio
3.96
dCas9/RNP to DNA ratio
not applicable
dev_stage
not applicable
geo_loc_name
synthetic construct
http://purl.obolibrary.org/obo/NCBITaxon_32630
organism
not applicable
sex
34
sgRNA input amount (ng)
0.3
sgRNA input concentration (uM)
3.564
sgRNA to DNA ratio
32630
strain
not applicable
tissue
enrichment E BC1.0 dhfr S2 SC 1384 target ProteinaseK 15min 500ng
title