1
Amount of dCas9 added (ul)
BAR-CAT 1.0
BAR-CAT Version Used
4994684
Barcode_Dropouts
20
Bead resuspension OR Elution vol (ul)
18
Cumulative wash volume (mL)
Supercoiled
DNA Library Format (Supercoiled vs. Linear)
500
DNA input amount (ng)
2
Enriched DNA volume (ul) used per qPCR
15
Enrichment incubaton time at 37 C (min)
12
Expected Number of Spacers in sgRNA library
SAMN48946972
External Id
0.61841
Fraction of Pop Not in Original Scrape
0.45991
Gini of Barcodes After Enrichment
University of Oregon
INSDC center name
2025-06-07T00:00:00Z
INSDC first public
2025-06-07T14:14:05.333Z
INSDC last update
SRS25298045
INSDC secondary accession
live
INSDC status
pEVBC8-DHFR-S4
Library Plasmid
5
Magnetic beads per enrichment (ul) before dilution
28.5525
Median Fold Enrichment (ignoring dropouts)
4.83554
Median Log2 Enrichment Score (ignoring dropouts)
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
9590686
Number of 20 nt BC reads per Llibrary
5300938
Number of Barcodes in DNA Library
931997
Number of Enriched Non-Target Barcodes
9
Number of Enriched Targeted Barcodes (Coverage?)
389
Number of Genes in DNA Library
4G
Number of Guanines Before Spacer
24
Number of PCR Cycles used to Amplify Enriched DNA
12
Number of Targeted Barcodes
12
Observed Number of Spacers (Coverage)
0.99995
Off-Targets Population Fraction Enrichment Score (all)
SRS25298045
SRA accession
3
Target_Dropouts
26.2189
Targets Population Fraction Enrichment Score
9
Total number of washes
3575776
Total reads/sequencing depth?
2
Wash volume per wash (mL)
not applicable
age
not applicable
collection_date
1
dCas9 Concentration (uM)
Proteinase K
dCas9 Denaturation
1.11
dCas9 to sgRNA ratio
3.96
dCas9/RNP to DNA ratio
not applicable
dev_stage
not applicable
geo_loc_name
synthetic construct
http://purl.obolibrary.org/obo/NCBITaxon_32630
organism
not applicable
sex
34
sgRNA input amount (ng)
0.3
sgRNA input concentration (uM)
3.564
sgRNA to DNA ratio
32630
strain
not applicable
tissue
enrichment H BC1.0 dhfr S4 SC 12 target ProteinaseK 15min 500ng 5primeGGGG
title