3
Amount of dCas9 added (ul)
BAR-CAT 1.0
BAR-CAT Version Used
4553102
Barcode_Dropouts
20
Bead resuspension OR Elution vol (ul)
18
Cumulative wash volume (mL)
Supercoiled
DNA Library Format (Supercoiled vs. Linear)
2500
DNA input amount (ng)
2
Enriched DNA volume (ul) used per qPCR
15
Enrichment incubaton time at 37 C (min)
12
Expected Number of Spacers in sgRNA library
SAMN48946965
External Id
0.65487
Fraction of Pop Not in Original Scrape
0.38738
Gini of Barcodes After Enrichment
University of Oregon
INSDC center name
2025-06-07T00:00:00Z
INSDC first public
2025-06-07T14:13:57.770Z
INSDC last update
SRS25298029
INSDC secondary accession
live
INSDC status
pEVBC8-DHFR-S4
Library Plasmid
5
Magnetic beads per enrichment (ul) before dilution
19.551
Median Fold Enrichment (ignoring dropouts)
4.28917
Median Log2 Enrichment Score (ignoring dropouts)
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
9590686
Number of 20 nt BC reads per Llibrary
5300938
Number of Barcodes in DNA Library
2162397
Number of Enriched Non-Target Barcodes
7
Number of Enriched Targeted Barcodes (Coverage?)
389
Number of Genes in DNA Library
1G
Number of Guanines Before Spacer
26
Number of PCR Cycles used to Amplify Enriched DNA
12
Number of Targeted Barcodes
12
Observed Number of Spacers (Coverage)
0.99998
Off-Targets Population Fraction Enrichment Score (all)
SRS25298029
SRA accession
5
Target_Dropouts
13.2668
Targets Population Fraction Enrichment Score
9
Total number of washes
5623803
Total reads/sequencing depth?
2
Wash volume per wash (mL)
not applicable
age
not applicable
collection_date
1
dCas9 Concentration (uM)
Proteinase K
dCas9 Denaturation
1.67
dCas9 to sgRNA ratio
2.376
dCas9/RNP to DNA ratio
not applicable
dev_stage
not applicable
geo_loc_name
synthetic construct
http://purl.obolibrary.org/obo/NCBITaxon_32630
organism
not applicable
sex
68
sgRNA input amount (ng)
0.3
sgRNA input concentration (uM)
1.4256
sgRNA to DNA ratio
32630
strain
not applicable
tissue
enrichment F BC1.0 dhfr S4 SC 12 target ProteinaseK 15min 2500ng DNA 3XdCas9 2XsgRNA
title