1
Amount of dCas9 added (ul)
BAR-CAT 0.3
BAR-CAT Version Used
141463
Barcode_Dropouts
20
Bead resuspension OR Elution vol (ul)
18
Cumulative wash volume (mL)
Supercoiled
DNA Library Format (Supercoiled vs. Linear)
500
DNA input amount (ng)
2
Enriched DNA volume (ul) used per qPCR
60
Enrichment incubaton time at 37 C (min)
3
Expected Number of Spacers in sgRNA library
SAMN48946954
External Id
0.09904
Fraction of Pop Not in Original Scrape
0.86557
Gini of Barcodes After Enrichment
University of Oregon
INSDC center name
2025-06-07T00:00:00Z
INSDC first public
2025-06-07T14:14:00.083Z
INSDC last update
SRS25298034
INSDC secondary accession
live
INSDC status
pEVBC3-RFP
Library Plasmid
5
Magnetic beads per enrichment (ul) before dilution
205.052
Median Fold Enrichment (ignoring dropouts)
7.67985
Median Log2 Enrichment Score (ignoring dropouts)
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
14641735
Number of 20 nt BC reads per Llibrary
306388
Number of Barcodes in DNA Library
665057
Number of Enriched Non-Target Barcodes
3
Number of Enriched Targeted Barcodes (Coverage?)
1
Number of Genes in DNA Library
2G
Number of Guanines Before Spacer
28
Number of PCR Cycles used to Amplify Enriched DNA
3
Number of Targeted Barcodes
3
Observed Number of Spacers (Coverage)
0.99841
Off-Targets Population Fraction Enrichment Score (all)
SRS25298034
SRA accession
0
Target_Dropouts
353.94
Targets Population Fraction Enrichment Score
9
Total number of washes
13000000
Total reads/sequencing depth?
2
Wash volume per wash (mL)
not applicable
age
not applicable
collection_date
1
dCas9 Concentration (uM)
Proteinase K
dCas9 Denaturation
1.11
dCas9 to sgRNA ratio
3.96
dCas9/RNP to DNA ratio
not applicable
dev_stage
not applicable
geo_loc_name
synthetic construct
http://purl.obolibrary.org/obo/NCBITaxon_32630
organism
not applicable
sex
34
sgRNA input amount (ng)
0.3
sgRNA input concentration (uM)
3.564
sgRNA to DNA ratio
32630
strain
not applicable
tissue
enrichment D BC0.3 rfp SC 3 target ProteinaseK 60min 500ng
title