1
Amount of dCas9 added (ul)
BAR-CAT 0.2
BAR-CAT Version Used
83414
Barcode_Dropouts
50
Bead resuspension OR Elution vol (ul)
18
Cumulative wash volume (mL)
Supercoiled
DNA Library Format (Supercoiled vs. Linear)
50
DNA input amount (ng)
5
Enriched DNA volume (ul) used per qPCR
15
Enrichment incubaton time at 37 C (min)
18
Expected Number of Spacers in sgRNA library
SAMN48946948
External Id
0.13638
Fraction of Pop Not in Original Scrape
0.58724
Gini of Barcodes After Enrichment
University of Oregon
INSDC center name
2025-06-07T00:00:00Z
INSDC first public
2025-06-07T14:13:52.397Z
INSDC last update
SRS25298018
INSDC secondary accession
live
INSDC status
pEVBC3-RFP
Library Plasmid
5
Magnetic beads per enrichment (ul) before dilution
19.4867
Median Fold Enrichment (ignoring dropouts)
4.28442
Median Log2 Enrichment Score (ignoring dropouts)
Model organism or animal
NCBI submission model
Model.organism.animal.1.0
NCBI submission package
14641735
Number of 20 nt BC reads per Llibrary
306388
Number of Barcodes in DNA Library
268362
Number of Enriched Non-Target Barcodes
18
Number of Enriched Targeted Barcodes (Coverage?)
1
Number of Genes in DNA Library
2G
Number of Guanines Before Spacer
22
Number of PCR Cycles used to Amplify Enriched DNA
18
Number of Targeted Barcodes
18
Observed Number of Spacers (Coverage)
0.99849
Off-Targets Population Fraction Enrichment Score (all)
SRS25298018
SRA accession
0
Target_Dropouts
22.6893
Targets Population Fraction Enrichment Score
9
Total number of washes
1675164
Total reads/sequencing depth?
2
Wash volume per wash (mL)
not applicable
age
not applicable
collection_date
1
dCas9 Concentration (uM)
None
dCas9 Denaturation
1.11
dCas9 to sgRNA ratio
39.6
dCas9/RNP to DNA ratio
not applicable
dev_stage
not applicable
geo_loc_name
synthetic construct
http://purl.obolibrary.org/obo/NCBITaxon_32630
organism
not applicable
sex
34
sgRNA input amount (ng)
0.3
sgRNA input concentration (uM)
35.64
sgRNA to DNA ratio
32630
strain
not applicable
tissue
enrichment C BC0.2 rfp SC 18 target
title