2025-06-16
ENA first public
ERC000011
ENA-CHECKLIST
Dipartimento di Medicina Molecolare e Biotecnologie Mediche Università degli studi di Napoli - Federico II
INSDC center name
public
INSDC status
ERS21137329
SRA accession
E-MTAB-14526:1a
Submitter Id
ArrayExpress
broker name
SUPM2
cell_line
neoplastic cell
cell_type
not collected
collection date
human
common name
Protocols: cell lines were from DSMZ (SUPM2, L82). MS-5 cells were kindly shared by Prof. Lucio Pastore (Department of Molecular Medicine and Medical Biotechnology, University of Napoli, Italy).  All cells were cultivated at 37°C in a humidified 5% CO2 atmosphere and were routinely tested for mycoplasma contamination using a Mycoplasma PCR detection kit (Applied Biological Materials Inc. abm # G238) and always tested negative. Drugs were purchased at MedChem express. All drugs were dissolved according to manufacturer’s instructions. Total RNA extraction from cells was performed using TRIZOL (Invitrogen) according to the manufacturer’s protocols.  Total RNA concentrations were determined by using NanoDrop spectrophotometer (Thermo Fisher) and their quality was assessed with the TapeStation 4150 (Agilent Technologies). After GEM generation, all the steps of cDNA synthesis, clean-up and amplification, DNA fragmentation, end-repair, adapter ligation and indexing PCR-amplification were done with the Chromium Single Cell 3‘ reagents following the manufacturer’s protocol while the DNA purification as well as the size selection steps were done with SPRIselect magnetic beads (Beckman Coulter, Brea, CA) as indicated by the 10X protocol. Finally, the concentration of each indexed library was determined by using Qubit dsDNA BR Assay (Thermo Fisher Scientific, USA) and their quality were assessed with the D1000 screentape on the TapeStation 4150 (Agilent Technologies, Santa Clara, CA).
description
ALCL
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
1a
title