2025-06-17
ENA first public
ERC000047
ENA-CHECKLIST
EMG
INSDC center name
public
INSDC status
ERS25048762
SRA accession
ERR13917544_concoct_46
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
metaspades_v3.15.5
assembly software
default
binning parameters
MGnify-genomes-generation-pipeline_v1.0.0
binning software
marine sediment
broad-scale environmental context
EBI-EMG
broker name
2017-04-19T00:00
collection date
65.83
%
completeness score
CheckM2_v1.0.1
completeness software
1.68
%
contamination score
This sample represents a Third Party Annotation (TPA) Metagenome-assembled genome assembled from the metagenomic run ERR13917544 of study ERP164333.
description
marine sediment
environmental medium
North Sea
geographic location (country and/or sea)
54.08762700
DD
geographic location (latitude)
7.96819100
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
sediment metagenome
isolation_source
protein degradation
local environmental context
sediment metagenome
metagenomic source
Candidatus Bathyarchaeota archaeon
organism
An incubation experiment was performed, investigating protein degradation by uncultivated Thermoplasmatota in marine anaerobic sediments of the Helgoland mud area. Slurry for the first generation was prepared in a 1:4 ratio of sediment and sulfate-free artificial sea water. The used sediment originated from core HE483 station 2-2, 45-70 cm and was retrieved by gravity coring in 2017. The incubation experiment was performed anoxically for two treatments: the control sample was amended with 20 mM sulfate (Na2SO4) and an antibiotics mix (D-cycloserin, kanamycin, vancomycin, ampicillin and streptomycin 50 mg/l for each), the protein amended sample was additionally amended with 1.86 g/l egg white protein. After 372 days 5 mL of the first generation incubations were transferred into anoxic Widdel medium. The incubation experiment was performed anoxically for two treatments in duplicates: the control samples were amended with 30 mM sulfate (Na2SO4) and an antibiotics mix (D-cycloserin, kanamycin, vancomycin, ampicillin and streptomycin 40 mg/l for each), the protein amended samples were additionally amended with 2.33 g/l egg white protein. Sampling of the second generation was performed after 98 and 157 days. DNA was extracted and the present and active archaeal community was identified by 16S rRNA gene sequencing.
project name
SAMEA116057598
sample derived from
Candidatus Bathyarchaeota archaeon
scientific_name
Illumina HiSeq 4000
sequencing method
multi-marker approach
taxonomic identity marker
Metagenome-assembled genome: ERR13917544_concoct_46
title