2025-06-18
ENA first public
ERC000011
ENA-CHECKLIST
Department of Biomedical Engineering, Vanderbilt University
INSDC center name
public
INSDC status
ERS25058362
SRA accession
E-MTAB-15239:TNF treated_1
Submitter Id
ArrayExpress
broker name
astrocyte
cell type
not collected
collection date
human
common name
Protocols: Astrocytes derived from iPSCs were detached from MatriGel coated plate with Accutase. Cells were then rinsed with PBS and incubated in Trizol (Thermo Fisher 15596026) for 10 minutes at room temperature. RNA was extracted using a Direct-zol RNA miniprep kit (Zymo R2050) according to the manufacturer’s instruction. This process was repeated two additional times from independent iPSC differentiations (biological n=3).  Raw sequencing reads were obtained for the 9paired-end samples.FASTQ reads were mapped to human genome (hg19) by HISAT2 (version 2.1.0)
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
 induced pluripotent stem cell
progenitor cell type
Homo sapiens
scientific_name
inflammatory cytokines
stimulus
TNF treated_1
title