2024-01-01
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland Molecular and Integrative Bioscience Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland
INSDC center name
public
INSDC status
ERS24379662
SRA accession
E-MTAB-15105:A006
Submitter Id
5
month
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Lgr5+ISCs were FACS sorted and lyzed in immediately in Trizol. Total RNA from sorted ISCs from dm-aKG or Ctrl treated mice(n=6) were isolated with Trizol. Total RNA was treated with DNase using the Heat&Run gDNA Removal(ArcticZymes) to remove residual DNA From the resulting RNA, RNA-seq libraries were made using the Universal RNA-Seq with NuQuant kit(Tecan). cDNA was generated using a mixture of random and poly(T) priming from 15-16 ng of Total RNA. The cDNA is then fragmented, followed by end repaired, adapter ligation, strand selection and ribodepletion using AnyDeplete mouse probes(Tecan). The final library amplification step was performed with 18x cycles of PCR.
description
adult
developmental stage
Lgr5-EGFP-IRES-CreERT2(+/-)
genotype
not collected
geographic location (country and/or sea)
PK6341A
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
small intestine
organism part
Mus musculus
scientific_name
female
sex
dm-aKG
stimulus
C57BL/6j
strain
A006
title