2025-06-19
ENA first public
ERC000011
ENA-CHECKLIST
ETH Zurich
INSDC center name
public
INSDC status
ERS24804341
SRA accession
E-MTAB-15175:PR8X31_1
Submitter Id
6
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Female 6-8 weeks old C57BL/6 mice (Janvier) were infected with 200 PFU of PR8 (A/PuertoRico/8/1934 (H1N1)) or X31 (a reassortant virus that contains the six internal segments from PR8 and the glycoprotein-encoding segments (HA/NA) from strain A/Aichi/1968 (H3N2)) (LD50 = 1250 PFU) intranasally (i.n.). the tibia and femur bones were flushed in ice-cold RPMI containing 10% FCS buffer with 10 ng/mL IL6 (216-16, Peprotech). An RBC lysis step was performed in 4 mL ammonium-chloride-potassium lysis buffer for 1 min at room temperature and subsequently inactivated with 26 mL RPMI containing 10% FCS. Cells were isolated via facs following the aforementioned experimental conditions 10x genomics 5' protocol was performed as according to manufacturers 10x genomics 5' protocol was performed as according to manufacturers
description
adult
developmental stage
influenza
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
B cell
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
bone marrow
organism part
Mus musculus
scientific_name
female
sex
C57BL/6J
strain
PR8X31_1
title