2024-01-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115162446
External Id
Institut Jacques Monod - Université Paris Cité-CNRS-
INSDC center name
2024-01-29T22:04:31Z
INSDC last update
public
INSDC status
ERS17944080
SRA accession
E-MTAB-13744:Sample 9
Submitter Id
ArrayExpress
broker name
not collected
collection date
brewer's yeast
common name
Protocols: rrp6Δ exponentially growing cells were treated with 1 μg/mL tunicamycin for 2h, washed and resuspended in Tm free medium. Samples were collected at t= -2h (Untreated), t = 0 (Tm 2 H), and t = 3 H (Wash 3 H) for analysis by RNA seq RNA was isolated from 10 OD of exponentially growing cells washed in MilliQ water and snap frozen. RNA was extracted with the Nucleospin RNA II Kit (Macherey-Nagel) mRNA sequencing libraries were prepared from polyA+ RNAs purified from 1 ug of total RNA using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations. A 150 bp read sequencing was Illumina sequenced with multiplexing. A mean of 17.7 ± 2.4 million passing Illumina quality filter reads was obtained for each of the samples. Library preparation was performed by Novogene (Novogene Co., Ltd).
description
rrp6Δ
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Saccharomyces cerevisiae
organism
Saccharomyces cerevisiae
organism
whole organism
organism part
Saccharomyces cerevisiae
scientific_name
Sample 9
title