2025-06-19
ENA first public
ERC000011
ENA-CHECKLIST
Instituto de Medicina Molecular - João Lobo Antunes, FMUL, University of Lisbon
INSDC center name
public
INSDC status
ERS18536086
SRA accession
E-MTAB-13930:Sample 10
Submitter Id
33
year
age
ArrayExpress
broker name
CD4-positive, alpha-beta memory T cell
cell_type
not collected
collection date
human
common name
Protocols: Thymocytes were isolated by Ficoll-Hypaque (GE Healthcare) from the cell suspensions obtained by thymic tissue manual dispersion. CD4SP Tregs and Tconvs were sort-purified based on the surface expression of CD4, CD8, CD27, CD25 and CD127 in FACS Aria III (BD Biosciences), reaching purities always above 95%. CD3 was intentionally not used to avoid possible signalling, but the sorting strategy was always further validated in parallel using CD3 and intracellular Foxp3 in a Fortessa flow cytometer (BD Biosciences). The staining protocols have been previously described (Nunes-Cabaço et al., 2010). Thymic samples were obtained during paediatric reconstructive cardiac surgery, using tissue that would be otherwise discarded from children without evidence of immunodeficiency or syndromatic diseases. Conversion between annotations was made with “biomaRt”.  RNA samples were extracted from cell pellets of sorted 600,000 tTregs and tTconvs, using the AllPrep DNA/RNA kit (QIAGEN) and following the manufacturer’s instructions.  Libraries were built selecting for polyadenylated RNA after depleting ribosomal fraction.
description
adult
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
BC3
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
1
replicate
Homo sapiens
scientific_name
female
sex
Sample 10
title