2025-06-20
ENA first public
ERC000022
ENA-CHECKLIST
THE UNIVERSITY OF NEW SOUTH WALES
INSDC center name
public
INSDC status
ERS25054466
SRA accession
BH-23_18S
Submitter Id
5.09
m
altitude
polar desert biome
broad-scale environmental context
2023-02-06
collection date
50
mm
depth
Bunger Hills soil from Background Transect, sample BH-23 (18S)
description
5.09
m
elevation
soil
environmental medium
Antarctica
geographic location (country and/or sea)
-66.2507534
DD
geographic location (latitude)
100.6030817
DD
geographic location (longitude)
Wilkes Land, Bunger Hills, Edgeworth David Field Camp
geographic location (region and locality)
ground soil
local environmental context
Illumina MiSeq (1 � 250 bp)
nucleic acid amplification
FASTDNA� SPIN Kit for Soil (MP Biomedicals, USA)
nucleic acid extraction
soil metagenome
organism
10
pH
APHA 4500 H+ � B (1:5)
pH method
1391F/EukBr
pcr primers
Microbial community of Bunger Hills, East Antarctica
project name
APHA 2510 B
salinity method
-80
�C
sample storage conditions
0.3
g
sample volume or weight for DNA extraction
soil metagenome
scientific_name
18S rRNA
target gene
V9 on 18S rRNA
target subfragment
Bunger Hills soil sample BH-23 (18S)
title
1.5
g/kg
total organic carbon
ALS standard method EP003
total organic carbon method
8
%
water content
Dried @ 105-110�C
water content method