2025-06-20
ENA first public
ERC000022
ENA-CHECKLIST
THE UNIVERSITY OF NEW SOUTH WALES
INSDC center name
public
INSDC status
ERS25054482
SRA accession
BH-13_16S
Submitter Id
2.72
m
altitude
polar desert biome
broad-scale environmental context
2023-02-06
collection date
410
mm
depth
Bunger Hills soil from Sample_Helipad Zone, sample BH-13 (16S)
description
2.72
m
elevation
soil
environmental medium
Antarctica
geographic location (country and/or sea)
-66.25012983
DD
geographic location (latitude)
100.6022529
DD
geographic location (longitude)
Wilkes Land, Bunger Hills, Edgeworth David Field Camp
geographic location (region and locality)
ground soil
local environmental context
Illumina MiSeq (2 � 250 bp)
nucleic acid amplification
FASTDNA� SPIN Kit for Soil (MP Biomedicals, USA)
nucleic acid extraction
soil metagenome
organism
6.6
pH
APHA 4500 H+ � B (1:5)
pH method
515F-Y/806RB
pcr primers
Microbial community of Bunger Hills, East Antarctica
project name
APHA 2510 B
salinity method
-80
�C
sample storage conditions
0.3
g
sample volume or weight for DNA extraction
soil metagenome
scientific_name
16S rRNA
target gene
V4 on 16S rRNA
target subfragment
Bunger Hills soil sample BH-13 (16S)
title
1
g/kg
total organic carbon
ALS standard method EP003
total organic carbon method
13.3
%
water content
Dried @ 105-110�C
water content method