2025-06-21
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113971165
External Id
UOM
INSDC center name
2023-07-03T10:13:40Z
INSDC last update
public
INSDC status
ERS15963069
SRA accession
E-MTAB-13138:SG5_GFP
Submitter Id
4 to 5
month
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Mammary tumors (~1 cm3) were dissected from 4 different double transgenic female MMTV-NIC; R26R-mT/mG mice along with their tail biopsies. Tumors were dissociated into single cells by collagenase treatment followed by trypsin and subsequent dispase digestions. Single cells were stained with Lineage antibodies against CD31, Ter110, and CD45 and then two cell subpopulations were FACS sorted from each tumor sample: Linneg;tdTOM+ (i.e Neuneg lineage) and Linneg;GFP+ (i.e Neupos lineage) fractions. Tumors were grown as spontaneous tumor model in vivo. Sorted cells were immediately frozen for genomic DNA isolation. Genomic DNA was isolated from sorted cells and tail biopsies by using the QIAmp Fast DNA Tissue kit (#51404, Qiagen) according to the manufacturer’s protocols. Sequencing libraries were generated using on-bead tagmentation chemistry with the Illumina® DNA Prep, (M) Tagmentation Kit (Illumina) according to the manufacturer’s protocol. Briefly, bead-linked transposomes were used to mediate the simultaneous fragmentation of gDNA (100-500ng) and the addition of Illumina sequencing primers. Next, reduced-cycle PCR amplification was used to amplify sequencing ready DNA fragments and to add the indices and adapters. Finally, sequencing-ready fragments were washed and pooled prior to paired-end sequencing.
description
adult
developmental stage
breast cancer
disease
MMTV-NIC; R26R-mT/mG
genotype
not collected
geographic location (country and/or sea)
Lin-neg, tdGFP-pos, Neu-pos
immunophenotype
2
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
mammary tumor
organism part
Mus musculus
scientific_name
female
sex
FVB-NJ
strain
SG5_GFP
title