2025-06-21
ENA first public
ERC000011
ENA-CHECKLIST
The second hospital of Jilin university
INSDC center name
public
INSDC status
ERS24832350
SRA accession
E-MTAB-15206:A3
Submitter Id
ArrayExpress
broker name
SW480
cell line
epithelial
cell type
not collected
collection date
human
common name
Protocols: Each group of 2x106 cells was used for eukaryotic mRNA sequencing Each group of 2x106 cells was used for eukaryotic mRNA sequencing, total RNA was provided from the cell samples, RNA concentration and purity were detected using Nanodrop2000, and then a whole-genome kit was used for RNA library construction. Use Oligo (dT) magnetic beads with polyA for base complementary pairing to isolate and enrich the mRNA. Then add fragmentation buffer to randomly fragment the mRNA. 300bp small fragments are sorted by magnetic beads and reverse transcribed into double fragments using a reverse transcription kit. Strand cDNA, fill it with End Repair Mix and connect to the adapter. After the above processing, the Illumina platform: i) library enrichment, PCR amplification cycles; ii) 2% agarose recovery; iii) TBS380 (Picogreen) machine; iv) cBot bridge PCR amplification to generate clusters; v) PE library, read length 2×150bp, sequenced.
description
adult
developmental stage
colorectal cancer
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
colon
organism part
Homo sapiens
scientific_name
A3
title