2025-06-23
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA113936644
External Id
Discovery and Translation Science Department Leeds Institute of Cardiovascular and Metabolic Medicine University of Leeds LIGHT Laboratories  Clarendon Way Leeds, LS2 9JT
INSDC center name
2023-06-23T11:15:21Z
INSDC last update
public
INSDC status
ERS15930805
SRA accession
E-MTAB-13110:Sample 4
Submitter Id
12
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Homozygous (Efcab4b-/-) and wild-type (Efcab4b+/+) WT mice were weighed at 8 weeks and 12 weeks where they were sacrificed. Liver tissue was placed in a tube with a metal lysis bead with 1 ml Trizol Reagent. Tubes were then placed in a tissue lyser and agitated at 26 Hz for 3 x 1 min sessions. The resultant liquid was transferred to 1.5 ml centrifuge tubes, 200 μl of phenol-chloroform were added and left to settle for 3 mins at room temperature, then spun at 12000g (4°C) for 15 mins. The supernatant (top aqueous phase) was transferred to new tubes and 500 μl of isopropanol were added to each tube and gently mixed before being left to stand for 10 mins. The samples were then spun for 15 mins at 12000g (4°C), and the supernatant discarded. The pellets were re-suspended in 1 ml of 75% ethanol (in dH2O) before a further spin at 8000g (4°C) for 5min. The supernatant was removed carefully, the pellet dried and then 20-50 μl of RNA-free dH2O was added to each sample which were then stored at -80°C. RNA was quantified using a NanoDrop®. The library preparation was constructed using the TruSeq Stranded mRNA kit (Illumina), following the manufacturer’s recommended protocol.
description
adult
developmental stage
Efcab4b-/-
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
liver
organism part
Mus musculus
scientific_name
male
sex
C57BL
strain
Sample 4
title