2025-06-28
ENA first public
ERC000011
ENA-CHECKLIST
Instituto de Neurociencias, CSIC-UMH
INSDC center name
public
INSDC status
ERS24937534
SRA accession
E-MTAB-15220:Gr66a_Fed_2
Submitter Id
5 to 7
day
age
ArrayExpress
broker name
neuron
cell type
not collected
collection date
fruit fly
common name
Protocols: Samples were collected after dissection in Dulbecco’s Phosphate Buffer Saline, then digested by an enzymatic mixture with papaine and collagenase, and finally sorted by FACS into RNA extraction buffer. Samples were dissected in cold Calcium- and Magnesium-free 1X Dulbecco’s phosphate buffered saline (DPBS), and transferred into LowBind tubes containing DPBS 0’1% bovine serum albumim (BSA). Then, samples were digested before procedure to FACS. Flies were growth in standard Iberin fly food in a 12 h light:12 h dark cycle at 25ºC until use. RNA was extracted by using the PicoArcturus (ThermoFisher, ref. 12204-01) according to manufacturer standard protocol. This procedure includes a serial centrifugation method which include washing buffer and 70% ethanol, to finally elude samples in the elution buffer provided. Libraries were prepared by the CRG (Genomic Research Centre, Barcelona, Spain) using an ultra-low RNA input protocol.
description
adult
developmental stage
Gr66a-Gal4>UAS-transTANGO
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Drosophila melanogaster
organism
Drosophila melanogaster
organism
brain
organism part
Drosophila melanogaster
scientific_name
mixed
sex
Fed
state
white
strain
Gr66a_Fed_2
title