2025-06-29
ENA first public
ERC000011
ENA-CHECKLIST
IFOM ETS - The AIRC Institute of Molecular Oncology
INSDC center name
public
INSDC status
ERS23277208
SRA accession
E-MTAB-14815:Abeta-3
Submitter Id
ArrayExpress
broker name
hippocampal neuron
cell_type
not collected
collection date
house mouse
common name
Protocols: Mouse primary neurons were processed after 48 hours for immunofluorescence, RNA extraction, and viability assay﻿. Maxwell® RSC simplyRNA Tissue Kit (AS134, Promega) For each sample, 500 ng of total RNA were used to generate a library of fragments using Illumina Stranded mRNA prep ligation kit. Oligo(dT) magnetic beads purify and capture the mRNA molecules containing polyA tails. The purified mRNA was fragmented and copied into first strand complimentary DNA (cDNA) using reverse transcriptase and random primers. In a second strand cDNA synthesis step, dUTP replaces dTTP to achieve strand specificity. The final steps added adenine (A) and thymine (T) bases to fragment ends and ligate adapters. The resulting products were purified and selectively amplified with 12 cycles of PCR to generate an indexed library of fragments. The library was quantified using Qubit 4.0 fluorimeter, checked on Agilent Bioanalyzer 2100
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
brain
organism part
Mus musculus
scientific_name
Abeta-3
title