2025-06-29
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA110448863
External Id
Cancer Research UK Beatson Institute
INSDC center name
2022-08-01T15:31:42Z
INSDC last update
public
INSDC status
ERS12546893
SRA accession
E-MTAB-12007:5A
Submitter Id
age
ArrayExpress
broker name
P13
cell_line
human
common name
Protocols: GBM patient-derived cell lines were obtained as described in \"Oudin, A. et al. Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors. STAR Protocols 2, 100534, doi:10.1016/j.xpro.2021.100534 (2021).\". Intracortical implantation of organoids in immunodeficient mice is performed to induce tumor growth in vivo and to establish PDOX models method was used. Animals must be housed in individually ventilated cages in a Specific Pathogen Free (SPF) facility, under controlled environment (temperature 22 ± 2°C, humidity between 45% and 65% and 12 h light / 12 h dark cycle) with free access to autoclaved and acidified water and irradiated food ad libitum. GBM naive cells were seeded at different densities in 6-well plates and cultured in Plasmax. 24h after seeding, media were renewed with fresh Plasmax, supplemented with vehicle or dexamethasone 0.1 uM, and cells were incubated for 72 hours at 21% O2. Cells were then harvested for RNA extraction. Samples were processed using the QIAshredder/RNeasy Mini Kit (Qiagen) Libraries for cluster generation and DNA sequencing were prepared using Illumina TruSeq RNA Library Preparation Kit. Quality and quantity of the DNA libraries were assessed on an Agilent 2200 TapeStation (D1000 screentape) and Qubit (Thermo Fisher Scientific), respectively.
description
adult
developmental stage
glioblastoma multiforme
disease
wild type genotype
genotype
in vivo via intracortical implantation until neoplasm formation
growth condition
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
brain
organism part
biological replicate 1
replicate
Homo sapiens
scientific_name
female
sex
5A
title