2025-06-30
ENA first public
ERC000011
ENA-CHECKLIST
GMU
INSDC center name
public
INSDC status
ERS25085996
SRA accession
E-MTAB-15234:NC_02
Submitter Id
ArrayExpress
broker name
spermatogonial stem cell-like cell
cell type
not collected
collection date
human
common name
Protocols: The cells were maintained in Dulbecco’s Modified Eagle Medium and Nutrient Mixture F-12 (DMEM/F-12; Gibco, USA), supplemented with 10% fetal bovine serum (FBS;ScienCell, USA), and 1% penicillin-streptomycin solution (NCM Biotech, China).  RNA was extracted from transfected SSC cells using Total RNA Extraction Reagent (Vazyme, Nanjing, China) according to the manufacturer's instructions.  The RNA extracted from the cells was initially subjected to quality control. mRNAs with poly(A) tails were enriched using oligo(dT) magnetic beads. These mRNAs were then randomly fragmented using divalent cations in a specified buffer, and library construction was performed following the NEB standard library construction protocol.
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
testis
organism part
Homo sapiens
scientific_name
NC_02
title