2025-06-30
ENA first public
ERC000011
ENA-CHECKLIST
UOM-BCF
INSDC center name
public
INSDC status
ERS20852370
SRA accession
E-MTAB-14341:jg28
Submitter Id
20
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Colon tissue was harvested and snap frozen in liquid nitrogen. Colon tissue was homogenised in lysing MatrixD tubes (MP biomedical) containing TRIzol (Invitrogen), using a bead mill homogeniser (Fisherbrand). RNA was extracted with chloroform and aqueous phase was precipitated with isopropyl alcohol. Supernatant was washed with 70% ethanol and the RNA pellet was resuspended in nuclease-free water. RNA libraries were prepared using standard Illumina protocols with TruSeq Stranded mRNA assay (Illumina)
description
adult
developmental stage
Bmal1flox
genotype
not collected
geographic location (country and/or sea)
naive
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
colon
organism part
Mus musculus
scientific_name
male
sex
C57Bl/6
strain
jg28
title