2025-06-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114568076
External Id
Department of Bioengineering University of Illinois
INSDC center name
2023-11-01T20:43:27Z
INSDC last update
public
INSDC status
ERS16602622
SRA accession
E-MTAB-13512:Apta01_12h_2
Submitter Id
ArrayExpress
broker name
Kasumi-1
cell_line
myeloid cell
cell_type
not collected
collection date
human
common name
Protocols: Cells were collected by centrifugation post experimental time points and stored and shipped at -80 C to the sequencing facility. Cells were grown in HPLM media supplemented with 10% fetal bovine serum with antibiotic-antimycotic solution. Aptamers were stored in 10 mM HEPES buffer (pH 7.2) containing 100 mM NaCl and 12 mM MgCl2 and cells were incubated in the same buffer during the treatment period of 6 h. RNA was extracted using Trizol following manufacturers protocol. Sequencing library was constructing using polyA enrichment PCR.
description
adult
developmental stage
acute myeloid leukemia
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
Apta01_12h_2
title