2025-06-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114308879
External Id
Institute of Molecular Genetics of the Czech Academy of Sciences
INSDC center name
2023-08-29T13:24:19Z
INSDC last update
public
INSDC status
ERS16297881
SRA accession
E-MTAB-13218:LLY
Submitter Id
ArrayExpress
broker name
not collected
collection date
zebrafish
common name
Protocols: The adult female zebrafish (identical in age, body weight, and gonadal status) were randomly divided into four experimental groups in different photic conditions: Normal: LD = 12H L:12H D, Treated: (i) continuous light for one week, LLW (7 days), (ii) for one month, LLM (30 days), and (iii) for one year, LLY (365 days). All the samplings were performed at same time (12.00 H) The ovary was removed by dissecting the body cavity respectively. Sample for whole transcriptome sequencing was stored in RNAlater (Life Technology, USA)], and at -80°C. Total RNA was isolated from the sample using Trizol (Invitrogen) method for RNA extraction. SENSE mRNA-Seq Library Prep Kit V2 (Lexogen, Austria) was used as per the protocol to generate the libraries.
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
continuous light for a year
growth condition
not applicable
isolate
Danio rerio
organism
Danio rerio
organism
ovary
organism part
Danio rerio
scientific_name
female
sex
LLY
title