2025-07-01
ENA first public
ERC000037
ENA-CHECKLIST
Treatment in minutes
Experimental Factor description
oxidddd----
Experimental Factor type
30
min
Experimental Factor values
CITA
INSDC center name
public
INSDC status
ERS22185638
SRA accession
Manzana_amarilla_de_octubre_30_3
Submitter Id
NaN
age
2021-11-04
collection date
apple
common name
RNA from apple pulp extracted from 'Manzana amarilla de octubre' at 30 mins of oxidation.
description
Manzana amarilla de octubre'
genotype
Spain
geographic location (country and/or sea)
41.68
DD
geographic location (latitude)
-0.66
DD
geographic location (longitude)
None
isolation and growth condition
fruit_pool
observation_unit_description
Malus domestica
organism
Apple
organism common name
PO:0025502
plant developmental stage
ENVO_00001998
plant growth medium
PO:0009001
plant structure
EO:0007161
plant treatment
Fruit
plant_anatomical_entity_name
Total RNA extraction from a pool of apple slices was performed using the CTAB protocol (Meisel 2005) RNA was treated with DNase using the TURBO DNA-freeTM kit (Invitrogen, Waltham, MA, USA), following the manufacturers� instructions. RNA quantity and purity were assessed with the Eukaryotic Total RNA Nanobioanalyzer Assay in a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).
protocol
3
replicate
Malus domestica
scientific_name
apina@cita-aragon.es
study_contact_email
Ana Pina Sobrino
study_contact_name
AL30E3
title
Field environment condition CO_715:0000185
type_of_growth_facility