2025-07-01
ENA first public
ERC000011
ENA-CHECKLIST
IMEX
INSDC center name
public
INSDC status
ERS24815486
SRA accession
E-MTAB-15193:Sample 7
Submitter Id
8
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: The trigeminal ganglia were dissected after euthanasia and cardiac perfusion with PBS to remove contaminating blood cells, collected in ice-cold TRI Reagent, and stored at -80°C until processing. both trigeminal ganglia from one mouse were homogenized in 1 ml of TRI Reagent, and then 0.2 ml of isopropanol was added. After centrifugation, RNA was purified from the aqueous phase using the Direct-zol RNA MiniPrep kit (Cat #R2052, Zymo Research, Irvine, CA, USA) following the manufacturer’s instructions. The concentration and purity of RNA were assessed with a NanoDrop 1000 spectrophotometer (ThermoFisher  Scientific, Waltham,  MA,  USA). RNA-Seq was performed by NovoGene (Sacramento, CA, USA) using the Illumina NovaSeq platform to generate 150 bp paired-end reads.
description
adult
developmental stage
dry eye syndrome
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
unilateral (right)
laterality
Mus musculus
organism
Mus musculus
organism
trigeminal ganglion
organism part
only contralateral (left) ganglion
sampling site
Mus musculus
scientific_name
female
sex
C57BL/6
strain
Sample 7
title