2025-07-01
ENA first public
ERC000037
ENA-CHECKLIST
Treatment in minutes
Experimental Factor description
oxidddd----
Experimental Factor type
30
min
Experimental Factor values
CITA
INSDC center name
public
INSDC status
ERS22185629
SRA accession
Borau-01_30_3
Submitter Id
NaN
age
2021-10-13
collection date
apple
common name
RNA from apple pulp extracted from 'Borau-01' at 30 mins of oxidation.
description
Borau-01'
genotype
Spain
geographic location (country and/or sea)
41.68
DD
geographic location (latitude)
-0.66
DD
geographic location (longitude)
None
isolation and growth condition
fruit_pool
observation_unit_description
Malus domestica
organism
Apple
organism common name
PO:0025502
plant developmental stage
ENVO_00001998
plant growth medium
PO:0009001
plant structure
EO:0007161
plant treatment
Fruit
plant_anatomical_entity_name
Total RNA extraction from a pool of apple slices was performed using the CTAB protocol (Meisel 2005) RNA was treated with DNase using the TURBO DNA-freeTM kit (Invitrogen, Waltham, MA, USA), following the manufacturers� instructions. RNA quantity and purity were assessed with the Eukaryotic Total RNA Nanobioanalyzer Assay in a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).
protocol
3
replicate
Malus domestica
scientific_name
apina@cita-aragon.es
study_contact_email
Ana Pina Sobrino
study_contact_name
AJ30E3
title
Field environment condition CO_715:0000176
type_of_growth_facility