2025-07-01
ENA first public
ERC000011
ENA-CHECKLIST
The University of Manchester
INSDC center name
public
INSDC status
ERS20608199
SRA accession
E-MTAB-14277:SB_G0_T0_rep1
Submitter Id
ArrayExpress
broker name
HES3
cell_line
not collected
collection date
human
common name
Protocols: Embryoid bodies on differentiation day 7.5 were dissociated to single cells by TrypLE Select and purified by FACS based on NKX2-5-GFP and SOX17-Tomato reporter status and immediately processed for ATAC-seq library preparation. ATAC-seq libraries were prepared using an ATAC-Seq Kit (Active Motif) as per the manufacturer’s instructions. Briefly, cells were gently washed with ice cold PBS and lysed with ATAC lysis buffer. Tagmentation master mix was prepared (Tagmentation buffer, PBS, 0.5% Digitonin, 10% Tween 20, Assembled Transposomes) and tagmentation carried out at 37oC for 30 minutes at 800 rpm. DNA was purified and then PCR amplified using unique combinations of i7 and i5 indexing primers per sample. After PCR amplification, samples were purified using SPRI beads and quality determined using Agilent Tapestation 2200. ATAC-seq libraries were prepared using an ATAC-Seq Kit (Active Motif) as per the manufacturer’s instructions.
description
cardiac differentiation
differentiation
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
embryonic stem cell
progenitor cell type
rep1
replicate
Homo sapiens
scientific_name
SB
stimulus
SB_G0_T0_rep1
title