2025-07-01
ENA first public
ERC000011
ENA-CHECKLIST
MPUSP
INSDC center name
public
INSDC status
ERS25085382
SRA accession
BKE5-F5
Submitter Id
Max Planck Unit for the Science  of Pathogens
collected_by
2021-03-22
collection date
Twenty-five ml of pooled gene deletion library (OD 0.4) were exposed to heat shock at 50�C for 2 hours in LB medium. One ml of culture was harvested at 15000 rpm for 3 minutes. The pellet was frozen in liquid nitrogen and stored at -80�C.
description
Germany
geographic location (country and/or sea)
Bacillus subtilis subsp. subtilis str. 168
organism
Bacillus subtilis subsp. subtilis str. 168
scientific_name
168
strain
Sequencing of barcodes of pooled gene deletion library in LB medium 2 hours after temperature shift to 50�C replicate 2
title