2025-07-02
ENA first public
ERC000011
ENA-CHECKLIST
Gyeongsang National University Animal Genomics Laboratory
INSDC center name
public
INSDC status
ERS20536016
SRA accession
E-MTAB-14232:guna
Submitter Id
5
year
age
ArrayExpress
broker name
not collected
collection date
dog
common name
Protocols: Eight beagle dogs (four males and four females) were investigated for sexual dimorphism in their transcriptomes. All dogs were five-year-old and had been neutered (castrated or spayed) before one year of age, and their healthy physical conditions were maintained through continuous monitoring, vaccination, and anthelmintic treatment. The experiment was ethically approved by the Institutional Animal Care and Use Committee of National Institute of Animal Science (NIAS 2022-586). Whole blood samples were collected using a 21-gauge needle and 5-mL syringe from the jugular vein of each dog. RNA from whole blood was extracted using a PAXgene Blood RNA Kit (762174; QIAGEN, Germantown, MD, USA) following the manufacturer’s instructions.  Libraries were prepared for 150 bp paired-end sequencing using a TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA).
description
adult
developmental stage
not collected
geographic location (country and/or sea)
not applicable
isolate
Canis lupus familiaris
organism
Canis lupus familiaris
organism
blood
organism part
Canis lupus familiaris
scientific_name
female
sex
guna
title