2025-07-03
ENA first public
ERC000011
ENA-CHECKLIST
University of Western Ontario
INSDC center name
public
INSDC status
ERS25120909
SRA accession
E-MTAB-15294:8CX
Submitter Id
9
month
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Mice were sacrificed at 9 months, brains were extracted on ice and dissected to obtain the prefrontal cortex from both hemispheres, tissue was snap frozen on dry ice then stored at -80 for up to 1 month. Nuclei were extracted from frozen tissues using the 10X Chromium Nuclei Isolation with RNase Inhibitor Kit according to directions. Isolated nuclei were suspended in buffer and run on a 10X Chromium to isolate individual nuclei and generate 3' tagged cDNA.  Brains were extracted and rapidly dissected on ice. The entire prefrontal cortex from both hemispheres was collected, and stored at -80 for up to 1 month. Mice were group housed, and fed standard chow and water ad libitum for 9 months then sacrificed and dissected. Mice were sacrificed during the light cycle. Nuclei were captured and lysed on the 10X Chromium, mRNA transcripts were polyA-selected, 3' tagged and reverse transcribed within nanodroplets. cDNA was collected from the 10X Chromium and converted to sequencing libraries using the 10X 3' scRNAseq Library Construction Kit.
description
adult
developmental stage
hMAPT,hAPP-NLF,hAPOE(E3)
genotype
not collected
geographic location (country and/or sea)
Mouse6
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
prefrontal cortex
organism part
Mus musculus
scientific_name
male
sex
C57B6
strain
8CX
title