2025-07-04
ENA first public
ERC000011
ENA-CHECKLIST
UOM-BCF
INSDC center name
public
INSDC status
ERS21379249
SRA accession
E-MTAB-14243_2:input_MEISKO_day5_exp1
Submitter Id
ArrayExpress
broker name
Nkx2-5 eGFP/w
cell_line
cardiac progenitor cell
cell_type
not collected
collection date
human
common name
Protocols: Cells were fixed with 1% HCHO for 10 minutes, neutralised with 0.125M Glycine for 5 min, washed twice with ice-cold PBS and spinned down. Cell pellets were snap frozen and stored at -80C. Embryonic stem (ES) cells were cultured on a monolayer of mitomycin inactivated mouse embryonic fibroblasts and maintained in DMEM/F12-based medium containing 20% knockout serum replacement (Invitrogen) and 10 ng/ml bFGF (Miltenyi Biotech). hPSCs were passaged using TrypLE Select (Invitrogen).  HESC NKX2-5eGFP/w and MEIS1/MEIS2 KO cell lines were differentiated using the embryoid body (EB) method as described in https://doi.org/10.1038/nmeth.1740. ChIPmentation samples and libraries were generated as described previously (https://www.doi.org/10.1016/j.xpro.2020.100187) with the use of the following antibodies: MEIS1 (Abcam ab19867; Active Motif MEIS1/2/3), GATA-6 (D61E4) XP Rabbit mAb (Cell Signaling Technology), H3K27ac (Abcam, ab4729) and rabbit polyclonal KMT2D (Atlas Antibodies). Purified DNA was amplified using Nextera indexes following manufacturer's protocol.
description
embryo stage
developmental stage
normal
disease
MEIS KO
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
inner cell mass
organism part
embryonic stem cell
progenitor cell type
Homo sapiens
scientific_name
female
sex
input_MEISKO_day5_exp1
title