2025-07-04
ENA first public
ERC000011
ENA-CHECKLIST
UOM-BCF
INSDC center name
public
INSDC status
ERS25138901
SRA accession
E-MTAB-15306:3RR_zt8_pre
Submitter Id
17
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Fecal pellets were collected and snap frozen. Once thawed, pellets were mixed with PBS and homogenised.  Feel pellets were collected from IEC-Bmal1-/- and Bmal1flox controls in naive state at ZT0, ZT4, ZT8, ZT12, ZT16, ZT20. Mice were then treated with 2% DSS. After 4 days of DSS, feel pellet collection was repeated. Microbial DNA was extracted with the DNeasy PowerSoil Pro Kit (Qiagen). Pre-amplification of the V4 region of 16S rRNA was performed using forward primer 5'-ACACTCTTTCCCTACACGACGCTCTTCCGAT-CTNNNNNGTGCCAGCMGCCGCGGTAA-3' (annealing sites in bold) and reverse primer 5'-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGGACTACHVGGGTWTCTAAT-3'. PhiX control v3 library (PhiX) was spiked into samples to balance low base diversity often found in microbiome samples.
description
adult
developmental stage
naïve
disease
wt
genotype
not collected
geographic location (country and/or sea)
naïve
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
feces
organism part
Mus musculus
scientific_name
male
sex
C57Bl/6
strain
3RR_zt8_pre
title