2025-07-04
ENA first public
ERC000011
ENA-CHECKLIST
CSIR-Centre for Cellular & Molecular Biology
INSDC center name
public
INSDC status
ERS22463355
SRA accession
E-MTAB-14641:C4_MEF_2H 30 Mins_S3
Submitter Id
ArrayExpress
broker name
MEF
cell_line
mouse embryonic fibroblast cell
cell_type
not collected
collection date
house mouse
common name
Protocols: Untreated and treated MEF cells were collected from 3 different 10cm plates for each group  RNA was extracted from MEF cells (three biological replicates) using RNeasy mini kit (QIAGEN, #74104). The quality and quantity of total RNA was checked using agarose gel and Qubit 4.0. After assessing the quality of RNA, 800-900 ng of total RNA was subjected to NEBNext® ultra™ directional RNA library prep kit for Illumina® (#E7420) using NEBNextPoly(A) mRNA magnetic isolation module (NEB #E7490). The QC of the prepared library was performed using High-Sensitivity Tape Station Kit (Agilent 2200, 5067-5585 (reagents) #5067-5584 ( screen tapes)) for fragment length distribution and Qubit dsDNA HS assay kit (Invitrogen, Q32851) for quantifying the library.
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
2H 30 Mins LLOMe S3
replicate
Mus musculus
scientific_name
2H 30 Mins LLOMe
stimulus
C4_MEF_2H 30 Mins_S3
title