2025-07-04
ENA first public
ERC000011
ENA-CHECKLIST
UOM-BCF
INSDC center name
public
INSDC status
ERS20598215
SRA accession
E-MTAB-14242:ATAC_day5_exp1
Submitter Id
ArrayExpress
broker name
Nkx2-5 eGFP/w
cell_line
cardiac progenitor cell
cell_type
not collected
collection date
human
common name
Protocols: Embryoid bodies were dispersed with TrypLE, cells were washed with ice-cold PBS, counted, aliquoted in 50000 to 100000 cells/aliquot and pelleted. Embryonic stem (ES) cells were cultured on a monolayer of mitomycin-inactivated mouse embryonic fibroblasts and maintained in DMEM/F12-based medium containing 20% knockout serum replacement (Invitrogen) and 10 ng/ml bFGF (Miltenyi Biotech). hPSCs were passaged using TrypLE Select (Invitrogen). HESC NKX2-5eGFP/w and MEIS1/MEIS2 KO cell lines were differentiated using the embryoid body (EB) method as described by in  https://doi.org/10.1038/nmeth.1740. Cell pellets lysis, tagmentation and DNA purification was performed using ATAC-Seq Kit (Active Motif) following manufacturer`s instructions. Purified DNA was amplified with 10 PCR cycles using Nextera indexes following ATAC-Seq Kit (Active Motif) protocol.
description
embryo stage
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
inner cell mass
organism part
embryonic stem cell
progenitor cell type
Homo sapiens
scientific_name
female
sex
ATAC_day5_exp1
title