2025-07-04
ENA first public
ERC000011
ENA-CHECKLIST
UOM-BCF
INSDC center name
public
INSDC status
ERS25138832
SRA accession
E-MTAB-15306:1R_zt12_dss
Submitter Id
8
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Fecal pellets were collected and snap frozen. Once thawed, pellets were mixed with PBS and homogenised.  Feel pellets were collected from IEC-Bmal1-/- and Bmal1flox controls in naive state at ZT0, ZT4, ZT8, ZT12, ZT16, ZT20. Mice were then treated with 2% DSS. After 4 days of DSS, feel pellet collection was repeated. Microbial DNA was extracted with the DNeasy PowerSoil Pro Kit (Qiagen). Pre-amplification of the V4 region of 16S rRNA was performed using forward primer 5'-ACACTCTTTCCCTACACGACGCTCTTCCGAT-CTNNNNNGTGCCAGCMGCCGCGGTAA-3' (annealing sites in bold) and reverse primer 5'-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGGACTACHVGGGTWTCTAAT-3'. PhiX control v3 library (PhiX) was spiked into samples to balance low base diversity often found in microbiome samples.
description
adult
developmental stage
DSS-treated
disease
wt
genotype
not collected
geographic location (country and/or sea)
DSS-treated
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
feces
organism part
Mus musculus
scientific_name
male
sex
C57Bl/6
strain
1R_zt12_dss
title