2025-07-04
ENA first public
ERC000011
ENA-CHECKLIST
UOM-BCF
INSDC center name
public
INSDC status
ERS20598028
SRA accession
E-MTAB-14241:input_day5_exp2
Submitter Id
ArrayExpress
broker name
Nkx2-5 eGFP/w
cell_line
embryonic stem cell
cell_type
cardiac progenitor cell
cell_type
not collected
collection date
human
common name
Protocols: Cells were fixed with 1% HCHO for 10 minutes, neutralised with 0.125M Glycine for 5 min, washed twice with ice-cold PBS and spinned down. Cell pellets were snap frozen and stored at -80C. Embryonic stem (ES) cells were cultured on a monolayer of mitomycin inactivated mouse embryonic fibroblasts and maintained in DMEM/F12-based medium containing 20% knockout serum replacement (Invitrogen) and 10 ng/ml bFGF (Miltenyi Biotech). hPSCs were passaged using TrypLE Select (Invitrogen). HOXB1_H11 cells were differentiated using the monolayer method as described in https://doi.org/10.1038/nmeth.1740. Doxycycline was added at day 4 of cardiac differentiation at 1 microgram/ml concentration. ChIPmentation samples and libraries were generated as described previously (https://www.doi.org/10.1016/j.xpro.2020.100187) with the use of the following antibody monoclonal ANTI-FLAG ® M2 antibody (Sigma-Aldrich) Purified DNA was amplified using Nextera indexes following manufacturer's protocol.
description
embryo stage
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
inner cell mass
organism part
Homo sapiens
scientific_name
female
sex
input_day5_exp2
title