2025-07-05
ENA first public
ERC000011
ENA-CHECKLIST
Calico Life Sciences LLC
INSDC center name
public
INSDC status
ERS25082535
SRA accession
E-MTAB-15273:Cerebellum_38
Submitter Id
4
month
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Mice were euthanized with CO2, and cerebellum, spinal cord, liver, kidney, lung, muscle and spleen were freshly collected and snap frozen in liquid nitrogen and stored at -80 ℃ 2BAct-maintained group: breeders were set up on 2BAct diet, experimental group maintained on 2BAct diet from birth into adulthood. 2BAct-withdrawal group: breeders were set up on 2BAct diet, experimental group maintained on 2BAct diet from birth until 4 months of age, then switched to control diet (0 mg/kg 2BAct) for 3 days prior to tissue collection. 2BAct-naive group: breeders set up on standard chow, experimental animals maintained on standard chow from birth to adulthood Tissues were treated with RNA-later ICE (Invitrogen #AM7030) for 24 hr at -20C and RNA extraction was performed following the MaxMAX total RNA isolation kit (Invitrogen #AM1830) RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA)
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
38
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Cerebellum
organism part
Mus musculus
scientific_name
male
sex
C57Bl/6N
strain
Cerebellum_38
title