2025-07-06
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114009071
External Id
Frontier Science Center for Stem Cell Research, Tongji University, Shanghai 200092, China.
INSDC center name
2023-07-10T15:19:58Z
INSDC last update
public
INSDC status
ERS15992937
SRA accession
E-MTAB-10588_2:C10_1
Submitter Id
ArrayExpress
broker name
MC38
cell_line
not collected
collection date
house mouse
common name
Protocols: Cell viability was detected with 0.4% trypan blue staining solution and counted with Countess II (Invitrogen) cell counter, and the cell viability was over 90%. RNA was extracted with TRIzol reagent (Ambion 15596-026)follow the instructions RNA was breaken into smart fragments with fragment buffer, and cDNAs were synthesized using the RNA fragments as templates, after which NEBNext Adaptor with hairpin loop structure were ligated ,and 3 µl USER Enzyme (NEB, USA) was used with purified adaptor-ligated cDNA at 37°C for 15 m in followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High -Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer.
description
colon carcinoma
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
colon
organism part
Mus musculus
scientific_name
C10_1
title