2025-07-06
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114004974
External Id
Frontier Science Center for Stem Cell Research, Tongji University, Shanghai 200092, China.
INSDC center name
2023-07-07T11:44:50Z
INSDC last update
public
INSDC status
ERS15988819
SRA accession
E-MTAB-10584_2:B39sub1
Submitter Id
ArrayExpress
broker name
MC38
cell_line
not collected
collection date
house mouse
common name
Protocols: The cells were washed once with TBS, then centrifuged at 4000g to discard the supernatant, added 10 times the volume of lysis buffer, and water bathed for 2 hours at 55℃. DNA extraction were performed follow the steps below: An equal volume of saturated phenol was added to the above sample treatment solution, then centrifuged at 5000g for 10 minutes. The upper aqueous phase was taked into a new tube, and repeat the step until the water phase was clear. Then 1/10 volume of 3M sodium acetate (PH5.2) and 2.5 times volume of absolute ethanol were added and centrifuged to discard the supernatant. Last 75% ethanol was used to wash for DNA extraction. For library Preparation, the genomic DNA was randomly broken into fragments about 180-280 bp in length by the Covaris sonicator. Then DNA fragments were end repaired and phosphorylated, T4 DNA ligase was used to ligate the Illumina sequencing adapter to both ends of the library DNA , and size selection was captured with magnetic beads. Captured libraries were enriched by PCR Amplification.
description
colon carcinoma
disease
Bap1 knockout
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
colon
organism part
Mus musculus
scientific_name
B39sub1
title