2025-07-06
ENA first public
ERC000013
ENA-CHECKLIST
SAMEA114003929
External Id
UNIVERSITY OF SZEGED
INSDC center name
2023-07-06T21:05:11Z
INSDC last update
public
INSDC status
ERS15987776
SRA accession
Can_fam_Toti_illumina_v3_4_20220629_invitrogen_I1_R2
Submitter Id
Quick-16S-AdapterFw-(TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG), Quick-16S-AdapterRev-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG and Illumina P5 5'-(AATGATACGGCGACCACCGA), P7 5'-(CAAGCAGAAGACGGCATACGAGAT)
adapters
Csongrad county
broad-scale environmental context
2021-12-01
collection date
Can_fam_Toti_illumina_v3_4_20220629_invitrogen_I1_R2
description
no
environmental medium
Hungary
geographic location (country and/or sea)
46.253
DD
geographic location (latitude)
20.14824
DD
geographic location (longitude)
13
years
host age
stool
host body product
Hungarian herding terrier: Pumi
host common name
omnivore
host diet
Harsanyi-Tekergo Bator
host family relationship
Canis lupus familiaris
host scientific name
male
host sex
cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host subspecific genetic lineage
9615
host taxid
Quick-16S NGS Library Prep Kit V3-V4
library construction method
PCR
library screening strategy
Szeged
local environmental context
3-(ACCTGGAT), 5-(TTCTAGAC)
multiplex identifiers
Quick-16S NGS Library Prep Kit V3-V4
nucleic acid amplification
PureLink Microbiome DNA Purification Kit, Invitrogen
nucleic acid extraction
gut metagenome
organism
initial denaturation:95degC_10min; denaturation=95degC_30sec; annealing=55deg_30sec; extension=72deg_3min
pcr conditions
341fw (CCTACGGGDGGCWGCAG, CCTAYGGGGYGCWGCAG), 806rev (GACTACNVGGGTMTCTAATCC)
pcr primers
Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes
project name
gut metagenome
scientific_name
software
sequence quality check
Illumina MiSeq v2 500 nano
sequencing method
16SrRNA
target gene
V3-V4
target subfragment
toti_illumina_v3_4_20220629_invitrogen_I1_R2
title