2025-07-06
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114009059
External Id
Frontier Science Center for Stem Cell Research, Tongji University, Shanghai 200092, China.
INSDC center name
2023-07-10T15:10:46Z
INSDC last update
public
INSDC status
ERS15992926
SRA accession
E-MTAB-10587_2:B39-3
Submitter Id
ArrayExpress
broker name
MC38
cell_line
not collected
collection date
house mouse
common name
Protocols: MC38-Bap1-Wildtype and MC38-Bap1-Knockout tumor cells expressing BFP fluorescent protein were injected into Rag2-/- mice subcutaneously. On the 10th day of tumor growth, the tumor cell was dissociated into single cells with Tumor Dissociation Kit (Miltenyi,130-096-730), and red blood cells were removed with ACK lysis buffer (Gibco,492-01). The dead cells in tumor cells were removed with Dead Cell Removal kit (Miltenyi,130-090-101), Then the tumor cells were resuspended with Facs buffer and BFP positive tumor cells were sorted by flow cytometry. Then RNA was extracted with TRIzol reagent (Ambion 15596-026)follow the instructions. RNA was breaken into smart fragments with fragment buffer, and cDNAs were synthesized using the RNA fragments as templates, after which NEBNext Adaptor with hairpin loop structure were ligated ,and 3 µl USER Enzyme (NEB, USA) was used with purified adaptor-ligated cDNA at 37°C for 15 m in followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High -Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer.
description
colon carcinoma
disease
Bap1 knockout
genotype
not collected
geographic location (country and/or sea)
injected into Rag2-deficient mice
growth condition
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
neoplasm
organism part
Mus musculus
scientific_name
B39-3
title