2025-07-06
ENA first public
ERC000013
ENA-CHECKLIST
SAMEA114003833
External Id
UNIVERSITY OF SZEGED
INSDC center name
2023-07-06T21:05:10Z
INSDC last update
public
INSDC status
ERS15987680
SRA accession
Can_fam_Toti_illumina_v1_3_20211216_qiagen_Q1_R2
Submitter Id
Perkin-Fw-(AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT), Perkin-Rev-(CAAGCAGAAGACGGCATACGAGATXXXXXXXXXXXX1GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT)
adapters
Csongrad county
broad-scale environmental context
2021-12-01
collection date
Can_fam_Toti_illumina_v1_3_20211216_qiagen_Q1_R2
description
no
environmental medium
Hungary
geographic location (country and/or sea)
46.253
DD
geographic location (latitude)
20.14824
DD
geographic location (longitude)
13
years
host age
stool
host body product
Hungarian herding terrier: Pumi
host common name
omnivore
host diet
Harsanyi-Tekergo Bator
host family relationship
Canis lupus familiaris
host scientific name
male
host sex
cross-breeding of Hungarian Puli with French and German herding dogs originated from 17. century
host subspecific genetic lineage
9615
host taxid
NEXTflex 16S V1 - V3 Amplicon-Seq Kit - 12
library construction method
PCR
library screening strategy
Szeged
local environmental context
TGACGCGCGGCC
multiplex identifiers
NEXTflex 16S V1 - V3 Amplicon-Seq Kit - 12
nucleic acid amplification
QIAamp Fast DNA Stool Mini Kit, Qiagen
nucleic acid extraction
gut metagenome
organism
initial denaturation:98degC_4min; denaturation=98degC_30sec; annealing=60deg_30sec; extension=72deg_20sec
pcr conditions
16S-V1-V3-Fw(CTCTTTCCCTACACGACGCTCTTCCGATCTAGAGTTTGATCCTGGCTCAG), 16S-V1-V3-Rev(CTGGAGTTCAGACGTGTGCTCTTCCGATCTGTATTACCGCGGCTGCTGG)
pcr primers
Comparative metagenomic profiling of canine gut microbial community using different sample preparation methodes
project name
gut metagenome
scientific_name
software
sequence quality check
Illumina MiSeq v2 500 nano
sequencing method
16SrRNA
target gene
V1-V3
target subfragment
toti_illumina_v1_3_20211216_qiagen_Q1_R2
title