2025-07-07
ENA first public
ERC000011
ENA-CHECKLIST
Duke-NUS Medical School
INSDC center name
public
INSDC status
ERS22605619
SRA accession
E-MTAB-14708:HZV020_D0
Submitter Id
63
year
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Blood was drawn from subjects into Tempus tubes and RNA extraction performed. RNA isolation from whole blood was performed using the Tempus Spin RNA Isolation Kit, eluted in 90uL elution buffer and stored at -80C. 1 μg total RNA was used for library preparation by Azenta Life Sciences. Poly(A) mRNA isolation was performed using Oligo(dT) beads and mRNA fragmentation was performed using divalent cations and high temperature. Priming was performed using random primers followed by synthesis of first strand and the second-strand cDNA. Purified cDNA was then treated to repair both ends and dA-tailing added, followed by a T-A ligation to add adaptors to both ends. Size selection of adaptor-ligated DNA was then performed using DNA Clean Beads. Each sample was then amplified by PCR using P5 and P7 primers and the PCR products were validated.
description
adult
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
HZV020
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
FR
responder
Homo sapiens
scientific_name
male
sex
HZV020_D0
title
Shingrix (RZV)
vaccine