2025-07-07
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Molecular Oncology, Philipps-University Marburg
INSDC center name
public
INSDC status
ERS20104326
SRA accession
E-MTAB-14099:LNCaP_ion_3
Submitter Id
ArrayExpress
broker name
LNCAP
cell_line
prostate gland cancer cell
cell_type
not collected
collection date
human
common name
Protocols: The human prostate cancer cell lines LNCaP and DU145 were obtained and authenticated from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Germany LNCaP, DSMZ-ACC 256; DU145, DSMZ-ACC 261).  Cells were cultured in accordance with the recommended conditions in RPMI full growth medium supplemented with fetal bovine serum.  LNCap and DU145 cells were treated by irradiations and LNCaP cells by addition of (5, 17)-17-hydroxy-androstane-3-one (di-hydro-testosterone; DHT) (20 nM) (Sigma-Aldrich). All treatments were performed in three independent triplicates with a calculated cell density of approximately 50% confluence at the time point of setup. For photon irradiation an X-RAD 320ix cabinet was used (X-ray unit (Precision X-Ray Inc, Denver, USA, 320 kV, 8 mA, ﬁlter: 0.5 mm Cu and 0.5 mm Al, dose rate 1 Gy/min).  12 C-irradiation was performed at the Marburg Ion-Beam Therapy Centre (MIT). Cells were irradiated with a horizontal beam of 114.5–129.5 MeV/n12 C-ions and positioned in the middle of a spread-out Bragg peak (SOBP) of 10–20 mm. Fields were applied using active scanning with a square of 324 cm2. RNA was isolated using the RNeasy Mini kit (Qiagen) and RNA quality was assessed using the Experion RNA StdSens Analysis Kit (Bio‐Rad).  RNAseq libraries were prepared from total RNA with the Lexogen QuantSeq 3'-mRNA Library Prep Kit FWD for Illumina (Lexogen, 015.24) in combination with the UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) (Lexogen, 081.96) following the manufacturer's protocol. Quality of sequencing libraries was controlled on a Bioanalyzer 2100 using the Agilent High Sensitivity DNA Kit (Agilent).
description
adult
developmental stage
prostate cancer
disease
TP53 wt/wt
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
prostate gland
organism part
Homo sapiens
scientific_name
LNCaP_ion_3
title