2025-07-09
ENA first public
ERC000011
ENA-CHECKLIST
INSERM U1170, Paris, France Gustave Roussy Institute, Villejuif, France Université Paris Saclay, France
INSDC center name
public
INSDC status
ERS20300401
SRA accession
E-MTAB-14190:CB2 DLX3 C
Submitter Id
ArrayExpress
broker name
Kit and Sca1-positive hematopoietic stem cell
cell_type
CD34+ cord blood cell
cell_type
not collected
collection date
human
common name
Protocols: CD34+ cord blood cells were transduced with lentivirus and sorted on GFP+ 4 days after. 5x104-1x105 GFP+ live-sorted cells were spun at 500g for 5 min, washed with cold DPBS, lysed in cold lysis buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, and 0.1% NP40, 3min on ice) and immediately spun at 500g for 10 min. The pelleted nuclei were resuspended in 50 µL of transposase reaction mix (Tagment DNA TDE1 Enzyme and Buffer Kit, Illumina) for 30 min at 39°C.  PCR purification was performed using Agencourt AMPure XP magnetic beads (Beckman Coulter A63880) in order to remove large fragments and remaining primers. Library quality was assessed using an Agilent 2100 Bioanalyzer using a High Sensitivity DNA chip (Agilent Technologies 5067-4626). Librairies were constructed by PCR using the Nextera DNA CD index kit (Illumina) and the NEBNext Ultra II Q5 master mix (NEB).
description
embryo
developmental stage
normal
disease
DLX3 overexpression
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
cord
organism part
3
replicate
Homo sapiens
scientific_name
CB2 DLX3 C
title