2025-07-09
ENA first public
ERC000011
ENA-CHECKLIST
INSERM U1170, Paris, France Gustave Roussy Institute, Villejuif, France Université Paris Saclay, France
INSDC center name
public
INSDC status
ERS20330300
SRA accession
E-MTAB-14204:in vivo EG (NSG_IL_429)
Submitter Id
ArrayExpress
broker name
induced pluripotent stem cell
cell_line
hematopoietic cell
cell_type
not collected
collection date
human
common name
Protocols: CD41+ Flow sorted differentiation day-13 hematopoietic cells from ctrl or EG iPSC, leukemic NSG mice or patient samples were collected. 5x104-1x105 GFP+ live-sorted cells were spun at 500g for 5 min, washed with cold DPBS, lysed in cold lysis buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, and 0.1% NP40, 3min on ice) and immediately spun at 500g for 10 min. The pelleted nuclei were resuspended in 50 µL of transposase reaction mix (Tagment DNA TDE1 Enzyme and Buffer Kit, Illumina) for 30 min at 39°C.  PCR purification was performed using Agencourt AMPure XP magnetic beads (Beckman Coulter A63880) in order to remove large fragments and remaining primers. Library quality was assessed using an Agilent 2100 Bioanalyzer using a High Sensitivity DNA chip (Agilent Technologies 5067-4626). Librairies were constructed by PCR using the Nextera DNA CD index kit (Illumina) and the NEBNext Ultra II Q5 master mix (NEB).
description
childhood acute megakaryoblastic leukemia
disease
CBFA2T3::GLIS2
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
pluripotent stem cell
progenitor cell type
1
replicate
Homo sapiens
scientific_name
in vivo EG (NSG_IL_429)
title