2025-07-10
ENA first public
ERC000011
ENA-CHECKLIST
Dipartimento di Medicina Molecolare e Biotecnologie Mediche (DMMBM), Federico II’ University of Naples
INSDC center name
public
INSDC status
ERS25118941
SRA accession
E-MTAB-15292:Sample 12
Submitter Id
ArrayExpress
broker name
PDX_MB002SU
cell line
not collected
collection date
house mouse
common name
Protocols: Fresh tumor tissues were resected under a stereoscope at endpoint. PDX tumor tissues were mechanically and enzymatically dissociated using a collagenase-based dissociation method. Approximately 10,000–16,000 tissue dissociated viable cells were loaded per sample on the Chromium Controller for encapsulation into gel beads-in-emulsion (GEMS) for reverse transcription of barcoded RNA.  Dynabead clean-up, PCR amplification and SPRIselect bead clean-up were carried out using the Chromium Single Cell 3' Gel Bead kit. Indexed single-cell libraries were generated using the Chromium Single Cell 3' Library kit and the Chromium i7 Multiplex kit.
description
medulloblastoma
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
tumor tissue
organism part
Mus musculus
scientific_name
Sample 12
title