2025-07-12
ENA first public
ERC000011
ENA-CHECKLIST
Centre for Experimental Medicine & Rheumatology, Queen Mary University of London
INSDC center name
public
INSDC status
ERS20877430
SRA accession
E-MTAB-14353:Sample 52
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: There were 52 water samples, 18 from drinking water treatment plants (DWTPs) and 34 from wastewater treatment plants (WWTPs).  We collected raw waters entering DWTP, waters subjected to oxidation after ozonation, raw waters taken from the inlet and outlet points of WWTPs, treated wastewater, and waters recycled through ultraviolet disinfection after treatment in WWTPs. All water samples were filtered through Whatman vacuum filtration. DNA extraction from Whatman filter papers was done using a spin column-based commercial nucleic acid extraction kit (Nucleogene Ltd., Kartal, Istanbul). This step was conducted using an Illumina DNA Prep kit (Illumina, USA, #20060059) and all kits were used according to the manufacturer's protocols. During library preparation, IDT for Illumina® DNA/RNA UD Indexes Set C (Illumina, USA, #20042666) adapters were used for sample indexing. The library was measured using a Qubit 4 Fluorometer and normalized before sequencing.
description
not collected
geographic location (country and/or sea)
not applicable
isolate
mixed sample
organism
mixed sample
organism
whole organism
organism part
freshwater sediment metagenome
sample type
Advanced
sampling site
mixed sample
scientific_name
Sample 52
title