2025-07-13
ENA first public
ERC000011
ENA-CHECKLIST
Rothamsted Research
INSDC center name
public
INSDC status
ERS20599582
SRA accession
E-MTAB-14268:Col replicate 1
Submitter Id
6
week
age
ArrayExpress
broker name
not collected
collection date
thale cress
common name
columbia
cultivar
Protocols: Unopened flower buds at floral stage 9 were collected in tubes, snap frozen in liquid nitrogen and stored at -80°C until further use. Plants were grown under long day conditions (16 hours light/8 hours dark) at 20°C. 100 mg of unopened flower buds was ground in liquid nitrogen and RNA was extracted using TRIzol reagent. Six micrograms of total RNA per sample was treated with DNase I. Four micrograms of total DNase I–treated total RNA was then treated with Ribo-Zero rRNA Removal Kit (MRZPL116, Epicentre) to deplete rRNA from the RNA sample. Fifty nanograms of rRNA-depleted DNase I–treated RNA was used to prepare RNA-seq libraries using the ScriptSeq v2 RNA-seq Library Preparation Kit (SSV21124, Epicentre)
description
whole plant flowering stage
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Arabidopsis thaliana
organism
Arabidopsis thaliana
organism
flower bud
organism part
Arabidopsis thaliana
scientific_name
Col replicate 1
title