2025-07-14
ENA first public
ERC000011
ENA-CHECKLIST
University of Toronto, St. Michael's Hospital
INSDC center name
public
INSDC status
ERS25089372
SRA accession
E-MTAB-15283:Ap9 no LPS 3
Submitter Id
ArrayExpress
broker name
RAW264.7
cell line
macrophage
cell type
not collected
collection date
house mouse
common name
Protocols: Lysates were prepared by washing cells twice with PBS+, lysed in recommended volume of RLT Lysis buffer from the Qiagen RNeasy RNA Isolation Kit (Cat. No. / ID: 74104) and collected by scraping with a sterile cell scraper. Lysates were vortexed for 20 seconds, centrifuged at max speed for 3 minutes at room temperature at stored at -80C for up to two weeks. RNA was isolated using Qiagen RNeasy RNA Isolation Kit (Cat. No. / ID: 74104) by following the manufacturer's instructions.  Library prep was performed using the NEB Ultra II Directional mRNA kit.
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
immune system
organism part
Mus musculus
scientific_name
none
stimulus
Ap9 no LPS 3
title