2025-07-15
ENA first public
ERC000011
ENA-CHECKLIST
Centre for Discovery Brain Sciences, University of Edinburgh
INSDC center name
public
INSDC status
ERS21390043
SRA accession
E-MTAB-14572:S5_265
Submitter Id
3 to 8
month
age
ArrayExpress
broker name
astrocyte
cell_type
not collected
collection date
house mouse
common name
Protocols: Single brain cells were isolated using Adult Brain Dissociation kit (Miltenyie, 130-107-677, Germany) as per the manufacturer’s instructions. The single brain cell suspension was then incubated with antibodies against CD31 (Biolegend, 102523, UK) CD11b (Biolegend, 101205, UK), ACSA2 (Miltenyie, 130-116-245, Germany), O4 (Miltenyie, 130-117-357, Germany), CD45 (Biolegend, 103125, Germany) for 15 minutes in ice, washed with PBS and then immediately FACS sorted into RNAprotect Cell Reagent (Qiagen, 76526, UK) under the gates of CD31+CD45-ACSA2- for brain endothelial cells, LY6G-CD11b+CD45low for microglia, ACSA+O4- for astrocytes. RNA extraction was carried out using the RNeasy Plus Micro Kit (Qiagen) as per the manufacturer’s instructions.  RNA-seq libraries were prepared by Edinburgh Genomics using the Illumina TruSeq stranded mRNA-seq kit, according to the manufacturer’s protocol (Illumina).
description
adult
developmental stage
astrocyte Nrf2 knockout
genotype
not collected
geographic location (country and/or sea)
S5_265
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
brain
organism part
Mus musculus
scientific_name
male
sex
saline
stimulus
C57BL/6
strain
S5_265
title