2025-07-17
ENA first public
ERC000011
ENA-CHECKLIST
University of Agriculture in Krakow, Poland
INSDC center name
public
INSDC status
ERS25143693
SRA accession
E-MTAB-15311:Sample 11
Submitter Id
43
day
age
ArrayExpress
broker name
not collected
collection date
barley
common name
BW084
cultivar
Protocols: Leaves from each mutant and Bowman cultivar were sampled after cold acclimation (CA) and after 24 h after de-acclimation (DA) in three biological replicates (leaves from three different plants). The leaves were immediately frozen in liquid nitrogen after sampling and stored at −80°C until use. Each sample was collected from the middle part of the youngest fully developed leaf and weighted 0.03–0.05 g. Plants were sown in pots (30 cm wide and 15 cm long) and grown for 3 weeks in a greenhouse at 18 °C (day/night) in daylight (the experiment was conducted in November in Krakow, Poland, 50°03’ N, 19°55’ E). Next, the plants were placed in a growth chamber for cold acclimation at a temperature of 2 °C/2 °C (day/night), with a photoperiod of 8 h/16 h (day/night) and 250 umol m-2 s-1 PPFD for 3 weeks. After cold acclimation, half of plants was de-acclimated at 12 °C/10 °C (day/night) 8 h/16 h (day/night) and 250 umol m-2 s-1 PPFD for 24 hours. Total RNA was isolated from 18 leaf samples using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). mRNA was isolated using oligo(dT)-attached magnetic beads, and cDNA libraries were constructed, converted into single-stranded circular DNA (ssCir DNA)
description
LP.04 four leaves visible stage
developmental stage
BW084-3
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Hordeum vulgare
organism
Hordeum vulgare
organism
leaf
organism part
Hordeum vulgare
scientific_name
De-acclimation
stimulus
Sample 11
title