2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
Rickard Sandberg lab, Department of Cell and Molecular Biology, Karolinska Institutet
INSDC center name
public
INSDC status
ERS23189400
SRA accession
E-MTAB-14822:Sample 1
Submitter Id
ArrayExpress
broker name
K562 or HEK293FT
cell_line
not collected
collection date
human
common name
Protocols: K562 cells: RPMI 1640 medium with 10% fetal bovine serum  and 1% Penicillin/Streptomycin at 37°C and 5% CO2, seeded to a density of 2*10^5 cells/ml and split 1:4 once the density approached 10^6 cells/ ml.  HEK293FT cells: complete DMEM medium with 4.5 g/l glucose, 6 mM L-glutamine, 10% fetal bovine serum, 0.1 mM MEM nonessential amino acids, 1 mM sodium pyruvate and 100 µg/ml Penicillin-Streptomycin, dissociated with TrypLE Express before re-seeding at 1:3 density every three to four days. During treatment, cells were kept in the incubator before they were spun down for 5 minutes at 300 RCF at the end of the incubation. RNA extraction was performed using the MGIEasy Nucleic Acid Extraction Kit. The sequencing libraries were prepared from isolated RNA through mRNA capture with an oligo-dT primer, and full length cDNA molecules were constructed through the use of a template switching oligo. The resulting cDNA fragments were pre-amplified through PCR and then fragmented through tagmentation to yield sequence ready fragments.
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
Sample 1
title