2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
SJTU
INSDC center name
public
INSDC status
ERS20302163
SRA accession
E-MTAB-14194:HepG2-shCtrl-m6A-IP-rep1
Submitter Id
ArrayExpress
broker name
HepG2
cell_line
liver cell
cell_type
not collected
collection date
human
common name
Protocols: HepG2 cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum. The cells were maintained at 37 °C in a 5% CO2 humidified atmosphere. When the cell density reached approximately 80%, the cells were collected. 1x10^7 HepG2 cells were used in shCtrl and YTHDC1 knockdown group. Total RNA was isolated by Trizol (Invitrogen) according to the manufacturer’s protocols. RNA was purified by NEBNext® rRNA Depletion Kit (NEB, USA). Fragmented and immunoprecipitated with anti-m6A antibody (Synaptic Systems, 202003). The RNA libraries were constructed using the NEBNext Ultra Directional RNA Library PrepKit (NEB, USA).
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
HepG2-shCtrl-m6A-IP-rep1
title