2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
Department of Biochemistry and Molecular Biology University of Southern Denmark
INSDC center name
public
INSDC status
ERS23770852
SRA accession
E-MTAB-14881:KO_RP_BAT3
Submitter Id
9-10
week
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: All mice were fasted two hours prior to sacrifice. Depending on subsequent experimental requirements mice were sacrificed by either cervical dislocation or anesthesia followed by heart puncture to draw blood. Anesthesia used: 25% hypnorm (0.315 mg fentanylcitrate, 10 mg of fluanisone, 0.5 mg methylparahydroxybenzoate and 1 mL sterile milliQ water) and 25% midazolam (Midazolam, 5 mg/mL) in sterile milliQ water, dosage: 0.01 mL/g mouse. EDTA plasma and tissues of interest were harvested and snap frozen in liquid nitrogen. Tissues were homogenized in TRI Reagent (Sigma) and total RNA was extracted in accordance with the manufacturer’s protocol. Sample preparation was performed as described in the NEBNext Poly(A) mRNA Magnetic Isolation Module kit and NEBNext Ultra II RNA Library Prep Kit E7770 (New England Biolabs, Ipswich, MA, USA) using 500ng input RNA. The amplified libraries were validated by Agilent 2100 Bioanalyzer using a DNA 1000 kit (Agilent Technologies, Inc., Santa Clara, CA, USA) and quantified by qPCR using the KaPa Library Kits (KaPa Biosystems, Wilmington, MA, USA).
description
adult
developmental stage
Adipoq-Acbp (-/-)
genotype
not collected
geographic location (country and/or sea)
2559
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
brown adipose tissue
organism part
Mus musculus
scientific_name
male
sex
C57BL/6J
strain
KO_RP_BAT3
title