2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
OXFORDUNIV
INSDC center name
public
INSDC status
ERS20598619
SRA accession
E-MTAB-14244:Sample 228
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Convalescent participants underwent a blood sample at least 28 days following their initial presentation and details regarding their clinical background, presentation and management were collected. Vaccinated participants had blood sampling 28 after their second dose of MVA-BN vaccination.  Single cells were directly sorted into 96-well PCR plates (Thermo Fisher Scientific) containing cell lysis buffer and stored at -80°C for further analysis. Reverse-transcription (RT) and PCR amplification were performed as described with the exception of using ISPCR primer with biotin tagged at the 5′ end and increasing the number of cycles to 25. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit
description
convalescent
disease staging
not collected
geographic location (country and/or sea)
MPX001
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood component
organism part
Homo sapiens
scientific_name
male
sex
Sample 228
title