2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
Rickard Sandberg lab, Department of Cell and Molecular Biology, Karolinska Institutet
INSDC center name
public
INSDC status
ERS23777837
SRA accession
E-MTAB-14924:Sample 1
Submitter Id
ArrayExpress
broker name
MCF-7
cell_line
not collected
collection date
human
common name
Protocols: MCF-7 cells were cultured at 50000 cells per well in 96-well plate overnight. Cells were treated for four hours with compounds from a panel of cancer drugs at a concentration of 10, 100, 1000, 10000 or 100000 micromolar for each compound in DMSO, or with only DMSO. Additionally, 200 micromolar 4sU was added four hours before cell harvest. RNA was isolated using magnetic beads following the BOMB-bio protocol 8.2 version 1.0 The sequencing libraries were prepared from isolated RNA through mRNA capture with an oligo-dT primer, and full length cDNA molecules were constructed through the use of a template switching oligo. The resulting cDNA fragments were pre-amplified through PCR and then fragmented through tagmentation to yield sequence ready fragments.
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
Sample 1
title