2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
Max Planck Institute of Immunobiology and Epigenetics (MPI-IE)
INSDC center name
public
INSDC status
ERS22468621
SRA accession
E-MTAB-13509_2:Ctrl_patient_1_2_3_4_5
Submitter Id
ArrayExpress
broker name
hematopoietic stem and progenitor cell
cell_type
healthy
clinical history
not collected
collection date
human
common name
Protocols: Human bone marrow was isolated from the sternum during surgery from patients who had experienced a myocardial infarction, as well as from control patients. The samples were subjected to Ficoll separation and subsequently frozen. On the day of sorting, the samples were thawed, and a total of 7,000 to 10,000 human hematopoietic stem and progenitor cells (HSPCs) (Lineage negative, CD38 negative, CD34 positive) were sorted in 2% BSA in PBS in two biological replicates. n/a The quality of the obtained cDNA and final libraries was assessed by capillary electrophoresis (Fragment analyzer, HS NGS Fragment Kit, Agilent).
description
adult
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
Pooled Ctrl patients 1-5
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
bone marrow
organism part
LineageNeg, CD38Neg, CD34Pos
phenotype
Homo sapiens
scientific_name
not available
sex
Ctrl_patient_1_2_3_4_5
title