2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
Unistra
INSDC center name
public
INSDC status
ERS22992033
SRA accession
E-MTAB-14786:PID666_Blood_1
Submitter Id
5
year
age
ArrayExpress
broker name
leukocyte
cell_type
not collected
collection date
human
common name
Protocols: Blood cells from standard venopuncture were treated with red blood cell lysis solution 1X (Miltenyi), washed and resuspended in PBS containing 2% fetal bovine serum to a concentration of ~800-1,000 cells/µl with high viability >95%.  The single-cell suspension was loaded at a volume to target of around 5000 cells per sample onto a Chromium Controller (10x Genomics, Pleasanton, CA) for Gel Bead-In-EMulsions “GEM” generation according to manufacturer’s instructions (10X Genomics, Pleasanton, CA). After GEM generation, single index libraries were generated using the Chromium Single Cell 3’ Library and Gel Bead kit v3.1, according to manufacturer’s instructions (PN 1000121, 10X Genomics, Pleasanton, CA). Size distribution and concentration of cDNA and final libraries were verified on an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies, Santa Clara, CA) and the concentration of the libraries were measured using Qubit Fluorometer (Thermo Fisher Scientific, Waltham, MA) and Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA).
description
adult
developmental stage
immunodeficiency disease
disease
not collected
geographic location (country and/or sea)
Patient _ Biol Replicate 1
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
male
sex
PID666_Blood_1
title