2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
OXFORDUNIV
INSDC center name
public
INSDC status
ERS20877852
SRA accession
E-MTAB-14360:05_VGTreated_Villi
Submitter Id
16
week
age
ArrayExpress
broker name
intestinal tissue
cell_type
not collected
collection date
house mouse
common name
Protocols: The proximal small bowel with dissected out and opened longitudinally.  The muscle layer was scraped off mechanically with a coverslip and discarded.  The villus compartment and crypt compartment were separated mechanically and digested using the MACS human umbilical cord dissociation kit (Miltenyi, 130-105-737) following the manufacturer’s instructions, with an enzymatic digestion time of 1h15m.  The 3 mice per group were pooled.  Cells were stained with propidium iodide (1:500, Invitrogen) and live cells were sorted using the BD FACSAriaTM Fusion Cell Sorter. Vil1-Grem1 mice were treated with a novel anti-Grem1 antibody (UCB Ab7326 mIgG, UCB Pharma), 30 mg/kg subcutaneously weekly from the age of 6-weeks old for 10 weeks. Nucleic acids were directly processed on intact cells using the Chromium Single Cell 3' Reagent v3 chemistry kit (10X Genomics) on a Chromium Instrument (10x Genomics). Libraries were prepared using the Chromium Single Cell 3' Reagent v3 chemistry kit (10X Genomics) on a Chromium Instrument (10X Genomics). Library quality was assessed using a TapeStation (Agilent Technologies).
description
adult
developmental stage
Vil1-Grem1
genotype
not collected
geographic location (country and/or sea)
pooled 2 females, 1 male
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
small intestine villi
organism part
Mus musculus
scientific_name
pooled 2 females, 1 male
sex
C57BL/6J
strain
05_VGTreated_Villi
title