2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
German Cancer Research Center
INSDC center name
public
INSDC status
ERS22930884
SRA accession
E-MTAB-14754:mitotic total RNA 2
Submitter Id
ArrayExpress
broker name
HeLa
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: HeLa cells were grown in normal conditions (DMEM high glucose medium, 10% FBS, 37°C, 5% CO2). 3 biological replicates were subjected to synchronization to prometaphse using double thymidine block and nocodazol treatment.  RNA extraction was performed using acidic phenol-chloroform-IAA mix and ethanol precipitation. The pellet was resuspended in nuclease free water. Library preparation occured at the DKFZ Core Facility using standard non-stranded library preparation after depletion of ribosomal RNA (Illumina RiboZero protocol).
description
adult
developmental stage
adenocarcinoma of cervix uteri
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
prometaphase REP2
growth condition
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
uterine cervix
organism part
Homo sapiens
scientific_name
mitotic total RNA 2
title