2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
Kyung Hee University
INSDC center name
public
INSDC status
ERS23769900
SRA accession
E-MTAB-14884:Wild-type2
Submitter Id
ArrayExpress
broker name
not collected
collection date
Japanese rice
common name
Dongjin
cultivar
Protocols: Mature anthers that include bicellular and tricellular stages of pollen were collected (11 to 13 stages of Anthers). Seeds of japonica rice cv. Dongjin was sterilized with 50% sodium hypochlorite and germinated in MS zero media in an incubator (14-h light/10-h dark, 28 °C (day)/22 °C (night), humidity 80%). After 1 week of germination, seedlings were transferred and grown in the greenhouse for 4 weeks under growth chamber conditions and transferred to the field. Mature anthers were frozen directly in Liquid nitrogen and finely ground using mortar. Grounded samples were homogenized using TRI reagents (RNAiso, Takara) and BCP (1-bromo-3-chloropropane). DNA, RNA, and protein layers were separated by centrifugation. Then, total RNA was extracted using the RNeasy Mini Kit (QIAGEN). The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, \"tagmentation\" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Oryza sativa Japonica Group
organism
Oryza sativa Japonica Group
organism
anther
organism part
Oryza sativa Japonica Group
scientific_name
Wild-type2
title