2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
EMBL
INSDC center name
public
INSDC status
ERS21039304
SRA accession
E-MTAB-14462:S25R2
Submitter Id
ArrayExpress
broker name
Schneider 2 cells
cell_line
Dm embryonic S2
cell_type
not collected
collection date
fruit fly
common name
Protocols: D. melanogaster S2 cells are cultured at 25°C in Schneider’s Drosophila medium (Gibco) supplemented with 10% of inactivated fetal bovine serum. For maintenance, cells were split every 3 to 5 days at ~1-2 million cells per mL for no more than 20 passages. Precision run-on sequencing (PRO-seq) was performed as previously described (Judd et al., 2020; Kwak et al., 2013) with slight modifications. For the Drosophila S2 cell samples, 10 million Drosophila S2 cells were used per sample with a spike-in of 0.1 million (1%) DNMT TKO mESCs. For the DNMT TKO mESC samples, 5 million DNMT TKO mESCs were used per sample with a spike-in of 0.05 million (1%) Drosophila S2 cells.
description
genetic modification
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Drosophila melanogaster
organism
Drosophila melanogaster
organism
Drosophila melanogaster
scientific_name
5
minute
time
S25R2
title
triptolide (10 mM)
treatment