2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
Fundación de Investigación Oftalmológica,
INSDC center name
public
INSDC status
ERS22590889
SRA accession
E-MTAB-14705:Sample 7
Submitter Id
ArrayExpress
broker name
Human fetal RPE
cell_type
not collected
collection date
human
common name
Protocols: After removing the culture media, lysis buffer was added on top of the cultures, then lysed cells were transferred to RNase-free eppendorf tubes.  The RNA of a total of 11 samples (3 biological replicates of each age, except for 25 weeks, with 2 biological replicates) was extracted using RNeasy Mini Kit (Qiagen), according to the manufacturer’s protocol mRNA enrichment and purification was performed using oligo(dT) coupled to magnetic beads. cDNA library construction involved mRNA fragmentation, cDNA synthesis (generating first-strand cDNA with random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis), end-repair, A-tailing and adapter ligation, followed by PCR amplification, circularization and rolling circle amplification for DNA nanoball (DNB) generation, which harbored more than 300 copies of one molecule.
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
Sample 7
title