2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
EMBL
INSDC center name
public
INSDC status
ERS21039313
SRA accession
E-MTAB-14462:TKO5R2
Submitter Id
ArrayExpress
broker name
mESC DNMT TKO 159
cell_line
mESC
cell_type
not collected
collection date
house mouse
common name
Protocols: Mouse ES cells were cultured on 0.2% gelatin-coated plates in ES medium (DMEM, supplemented with 15% FBS, LIF, 2-Mercaptoethanol, 2 mM L-Glutamine and 1x non-essential amino acids) at 37C and 5% CO2. Medium was changed daily and cells were split every second day. Precision run-on sequencing (PRO-seq) was performed as previously described (Judd et al., 2020; Kwak et al., 2013) with slight modifications. For the Drosophila S2 cell samples, 10 million Drosophila S2 cells were used per sample with a spike-in of 0.1 million (1%) DNMT TKO mESCs. For the DNMT TKO mESC samples, 5 million DNMT TKO mESCs were used per sample with a spike-in of 0.05 million (1%) Drosophila S2 cells.
description
gene knock out
genetic modification
dnmt1-/-, dnmt3a-/-, dnmt3b-/-
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Mus musculus
scientific_name
5
minute
time
TKO5R2
title
triptolide (10 mM)
treatment