2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
University of Florence Department of Agriculture, Food, Environment and Forestry (DAGRI)
INSDC center name
public
INSDC status
ERS20227905
SRA accession
E-MTAB-14165:GATuRep2
Submitter Id
30
day
age
ArrayExpress
broker name
not collected
collection date
European raspberry
common name
GlenAmple
cultivar
Protocols: Developing fruits of the raspberry cultivars ‘Anitra’, ‘Glen Ample’, ‘Varnes’ and ‘Veten’ were collected at three maturity stages; (1) unripe (at 25 days post-anthesis), (2) turning (at 30 days post anthesis), and (3) fully mature (35 days post anthesis). Fruit samples from each cultivar at each maturity stage were divided into 3 biological replicates, each with 15 berries with stage-representative characteristics. The berries were frozen in liquid nitrogen and kept at – 80 °C until further processing. The plants were maintained in a growth room at a constant temperature of 24 °C during the day and 18 °C at night, with a 16-hour photoperiod Tissue samples were ground to a fine powder under liquid nitrogen, and total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen, Oslo, Norway) following the manufacturer’s instructions. The concentration and purity of the resultant RNA was measured using a QIAxpert spectrophotometer (Qiagen, Oslo, Norway) and the integrity of the RNA was determined using a Qubit 4.0 fluorimeter (Thermo Fisher Scientific, Dartford, UK). Samples with an RNA integrity number (RIN) value above 7.0 were submitted for subsequent library preparation and sequencing. Library preparation was performed for the 36 fruit samples using the NEB Next® ultra RNA library prep kit (Biolabs, Inc., Beijing, China).
description
whole plant fruit development stage
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Rubus idaeus
organism
Rubus idaeus
organism
fruit
organism part
turning ripe
phenotype
Rubus idaeus
scientific_name
GATuRep2
title