2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
TVP Lab, WT/MRC Institute of Metabolic Science, University of Cambridge, United Kingdom
INSDC center name
public
INSDC status
ERS22181965
SRA accession
E-MTAB-14609:A6
Submitter Id
Batch2
batchid
ArrayExpress
broker name
PBAT
cell_line
Mature brown adipocyte
cell_type
not collected
collection date
house mouse
common name
Protocols: At the end of the treatment time, the cells were washed with phosphate-buffered saline (PBS) and snap-frozen on dry ice. Frozen cells were stored at -80°C until RNA extraction. The extraction was performed using the RNeasy Plus Mini Kit (Qiagen, 74104), according to the manufacturer's instructions. The cells underwent lysis in 350 μl of RLT buffer supplemented with 1% (v/v) β-mercaptoethanol (BME).  RNA libraries were prepared for sequencing by Novogene, processed to construct libraries suitable for the RNASeq analysis and following the standard protocols for RNA library construction. RNA integrity was confirmed by Bioanalyser analysis.
description
Mature adipocyte
developmental stage
9
day
differentiation
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
brown adipose tissue
organism part
Mus musculus
scientific_name
N/A
sex
BL6/J
strain
A6
title
BMP4
treatment