2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
EMBL
INSDC center name
public
INSDC status
ERS21039293
SRA accession
E-MTAB-14461:SMF_MM_DE_AMP_Plate4_DMSO_R2
Submitter Id
ArrayExpress
broker name
mESC DNMT TKO 159
cell_line
mESC
cell_type
not collected
collection date
house mouse
common name
Protocols: Mouse ES cells were cultured on 0.2% gelatin-coated plates in ES medium (DMEM, supplemented with 15% FBS, LIF, 2-Mercaptoethanol, 2 mM L-Glutamine and 1x non-essential amino acids) at 37C and 5% CO2. Medium was changed daily and cells were split every second day. Dual-enzyme SMF was performed following the protocol from Kleinendorst et al., 2021; Soenmezer et al., 2021.
description
gene knock out
genetic modification
dnmt1-/-, dnmt3a-/-, dnmt3b-/-
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Mus musculus
scientific_name
30
minute
time
SMF_MM_DE_AMP_Plate4_DMSO_R2
title
DMSO (500 nM)
treatment