2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
Kyung Hee University
INSDC center name
public
INSDC status
ERS18297062
SRA accession
E-MTAB-13830:Wildtype3
Submitter Id
ArrayExpress
broker name
not collected
collection date
Japanese rice
common name
Dongjin
cultivar
Protocols: Leaf samples from three independent replicates of both WT and OsABCB24 overexpression lines were processed.  Dongjin cultivar of Oryza sativa subspecies japonica served as the wild type (WT). All transgenic lines being developed on the genetic background of Dongjin. Seedlings were germinated on Murashige and Skoog medium for 10 days and transferred to soil in a greenhouse or paddy field. The greenhouse conditions were maintained at 28°C with 16 hours of light and 22°C with 8 hours of darkness, and humidity of 70%.  Leaf samples were frozen directly in Liquid nitrogen and finely ground. Grounded samples were homogenized using TRI reagents (RNAiso, Takara) and BCP (1-bromo-3-chloropropane). DNA, RNA, and protein layers were separated by centrifugation. Then, total RNA was extracted using the RNeasy Mini Kit (QIAGEN). The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, \"tagmentation\" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Oryza sativa Japonica Group
organism
Oryza sativa Japonica Group
organism
leaf
organism part
Oryza sativa Japonica Group
scientific_name
Wildtype3
title