2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
University of Southern Denmark
INSDC center name
public
INSDC status
ERS20948753
SRA accession
E-MTAB-14414:CX_4945_5
Submitter Id
ArrayExpress
broker name
3T3-L1
cell_line
small adipocyte
cell_type
not collected
collection date
house mouse
common name
Protocols: Cells were washed in PBS and harvested for RNA extraction 7 days after adipogenesis induction. Harvesting was done in duplicates in 3 separate experiments. Cells were cultured at 37°C, 5% CO2, in DMEM medium (Gibco, Invitrogen, Taastrup, Denmark) supplemented with fetal bovine serum (10%), and penicillin (100 U/ml)/streptomycin (100 µg/ml). A differentiation cocktail consisting of 0.5 mM 3-isobutyl-1-methylxanthine (IBMX, Sigma-Aldrich, Brøndby, Denmark), 1 µM Dexamethasone (Dex, Sigma-Aldrich), and 5 µg/ml Insulin (Ins, Sigma-Aldrich) was added to confluent cells at day 0. Fresh medium containing 10% FBS and 5 µg/ml Insulin was added to the cells after additional 72 hrs (i.e. day 3). Cells were subsequently supplemented with fresh growth medium on day 5. Where indicated, cells were additionally treated with vehicle (DMSO) or 10 µM CX-4945 (Silmitasertib, Selleck Chemicals, Houston, TX, USA) starting on day 0. Sample preparation was performed as described in the NEBNext ultra II RNA library prep kit (Illumina). The amplified libraries were validated by Agilent 2100 Bioanalyzer using a DNA 1000 kit from Agilent Technologies and quantified by qPCR using the KaPa Library Kits (KaPa Biosystems, Wilmington, MA, USA). Sample preparation was performed as described in the NEBNext ultra II RNA library prep kit (Illumina, San Diego, CA, USA). The amplified libraries were validated by Agilent 2100 Bioanalyzer using a DNA 1000 kit (Agilent Technologies, Inc., Santa Clara, CA, USA) and quantified by qPCR using the KaPa Library Kits (KaPa Biosystems, Wilmington, MA, USA).
description
mouse embryo stage
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
adipose tissue
organism part
Mus musculus
scientific_name
CX_4945_5
title