2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
ERIBA
INSDC center name
public
INSDC status
ERS23086808
SRA accession
E-MTAB-14808:MDA-MB-231 shFTO RNA-seq repl 1
Submitter Id
ArrayExpress
broker name
MDA-MB-231
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: Cells were grown as usual and harvested at 80% confluency in ice cold PBS with 100 μg/ml cycloheximide. RNA was isolated from a portion of cells processed for ribosome profiling using the RNeasy kit (Qiagen).  mRNA was isolated by NEXTFLEX® Poly(A) Beads (PerkinElmer) and quality checked by RNA 6000 nano chip (Agilent) on a Bioanalyzer 2100 (Agilent). Sequencing libraries were prepared using NEXTFLEX® Rapid Directional qRNA-Seq™ Library Prep Kit (PerkinElmer) according to the manufacturer’s instructions. Library quality was confirmed using a Bioanalyzer 2100 (Agilent) with the DNA 7500 kit (Agilent) and libraries were quantified by Qubit dsDNA BR assay (ThermoFisher).
description
adult
developmental stage
breast adenocarcinoma
disease
FTO knockdown
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
pleural effusion
metastatic site
Homo sapiens
organism
Homo sapiens
organism
breast
organism part
1
replicate
Homo sapiens
scientific_name
MDA-MB-231 shFTO RNA-seq repl 1
title