2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
Centre for Discovery Brain Sciences, University of Edinburgh
INSDC center name
public
INSDC status
ERS23862097
SRA accession
E-MTAB-14978:CTR3
Submitter Id
ArrayExpress
broker name
Innoprot P10361-IM
cell line
cerebrovascular endothelial cell
cell type
not collected
collection date
human
common name
Protocols: Human Brain Microvascular Endothelia Cells (BECs) were purchase from Innoprot (P10361-IM, Elexalde Derio, Spain). All plates and dishes for endothelial cells culture were coated with 0.2% Gelatine (G9391, Sigma-Aldrich) and 10ug/mL fibronectin (F1141-5MG, Sigma-Aldrich) for 30 min at 37 °C. Cells were cultured in Endothelial Cell Growth Medium with premixed supplements (C22022, Promocell) and 1% antibiotic-antimycotic solution (11570486, Gibco). RNA extraction was carried out using the RNeasy Plus Micro Kit (Qiagen) as per the manufacturer’s instructions. Libraries were prepared by Edinburgh Genomics using the Illumina TruSeq stranded mRNA-seq kit, according to the manufacturer’s protocol (Illumina).
description
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood vessel
organism part
Homo sapiens
scientific_name
Control
stimulus
CTR3
title