2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
Research Institute for Farm Animal Biology (FBN) Physiological Genomics Unit
INSDC center name
public
INSDC status
ERS20963102
SRA accession
E-MTAB-14436:N146
Submitter Id
24
week
age
ArrayExpress
broker name
not collected
collection date
chicken
common name
Protocols: The animal trial was performed at the Agricultural Experimental Station of the University of Hohenheim, Germany. The experimental protocol is in strict compliance with the German Animal Welfare Legislation and approved by the Regierungspräsidium Tubingen, Germany, and the Animal Welfare Committee of the University of Hohenheim (Project no. HOH67-21TEr). The hens were fed the experimental diets for a period of four weeks before slaughtering in week 19 (n = 40) and week 24 (n = 40), respectively. Kidney samples (n = 80) were dissected, snap-frozen in liquid nitrogen and stored in -80 °C. Before RNA extraction, whole frozen kidney samples were ground with pestle and mortar while submerged in liquid nitrogen. About forty grams of each kidney sample were used for RNA isolation using TRI reagent (Sigma-Aldrich, Taufkirchen, Germany) and phenol-chloroform-based RNA extraction according to the TRI reagent protocol. To ensure the absence of DNA, the isolated RNA was treated with DNase I (Roche Diagnostics, Mannheim, Germany) and finally purified using the NucleoSpin RNA extraction kit (Macherey-Nagel, Düren, Germany). The RNA concentration was determined through spectrophotometry using the NanoDrop ND-2000 (Peqlab, Erlangen, Germany) and RNA quality of final RNA was analyzed with the Bioanalyzer 2100 device (Agilent Technologies, Waldbronn, Germany).  The range of RNA integrity numbers (RIN) was between 6.4 and 8.9, with an average of 7.8. The absence of genomic DNA contamination was confirmed with polymerase chain reaction (PCR) specific for a chicken GAPDH fragment. RNA libraries were prepared using the Illumina Stranded mRNA preparation kit (Illumina, San Diego, CA, USA).
description
mature
developmental stage
reduced dietary phosphorus
diet
wild type genotype
genotype
not collected
geographic location (country and/or sea)
NA
individual
not applicable
isolate
Gallus gallus
organism
Gallus gallus
organism
kidney
organism part
Gallus gallus
scientific_name
female
sex
Lohmann Brown
strain
N146
title