2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
Kyung Hee University
INSDC center name
public
INSDC status
ERS23770583
SRA accession
E-MTAB-14885:ABCGmulti_mutant-3
Submitter Id
ArrayExpress
broker name
not collected
collection date
Japanese rice
common name
DongJin
cultivar
Protocols: Mature anthers that include bicellular and tricellular stages of pollen were collected (11 to 13 stages of Anthers). As this transgenic line exhibits complete sterility and fails to produce seeds, the plants analyzed in this study were T0 lines generated through tissue culture. These seedlings were maintained under growth chamber conditions in a greenhouse for four weeks prior to their transplantation into the paddy field. Mature anthers were frozen directly in Liquid nitrogen and finely ground using mortar. Grounded samples were homogenized using TRI reagents (RNAiso, Takara) and BCP (1-bromo-3-chloropropane). DNA, RNA, and protein layers were separated by centrifugation. Then, total RNA was extracted using the RNeasy Mini Kit (QIAGEN). The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, \"tagmentation\" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.
description
OsABCG16 (LOC_Os06g51460) and OsABCG28 (LOC_Os11g22350) double mutant
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Oryza sativa Japonica Group
organism
Oryza sativa Japonica Group
organism
anther
organism part
Oryza sativa Japonica Group
scientific_name
ABCGmulti_mutant-3
title