2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
German Cancer Research Center (DKFZ)
INSDC center name
public
INSDC status
ERS21087014
SRA accession
E-MTAB-14472:iCLIP2_KIFC1_REP2
Submitter Id
ArrayExpress
broker name
HeLa
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: HeLa cells were grown in normal conditions (DMEM high glucose medium, 10% FBS, 37°C, 5% CO2). 4 biological replicates were subjected to the iCLIP procedure with UV-crosslinking at 254 nm (200 mJ/cm2). We performed iCLIP2 protocol (Buchbender et al, 2020, methods 178:33-48) using anti-KIFC1 antibodies after cell lysis. After immunoprecipitation, the samples were run on a gel and transferred on a membrane. The membrane was cut at 90 to 150 kDa to 4-5 small pieces and transferred into a 2 ml low-bind tube for digestion with proteinase K. Further, RNA extraction was performed using acidic phenol-chloroform-IAA mix and ethanol precipitation. The pellet was resuspended in nuclease free water. For library preparation, we followed the iCLIP2 protocol as detailed in the publication Buchbender et al, 2020, methods 178:33-48 doi: 10.1016/j.ymeth.2019.10.003
description
adult
developmental stage
adenocarcinoma of cervix uteri
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
uterine cervix
organism part
Homo sapiens
scientific_name
iCLIP2_KIFC1_REP2
title