2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
Wageningen University and Research
INSDC center name
public
INSDC status
ERS20530065
SRA accession
E-MTAB-14229:Sample 1
Submitter Id
ArrayExpress
broker name
iPSC derived cell line
cell_line
somatic cell
cell_type
not collected
collection date
human
common name
Protocols: The total RNA was isolated from ChP organoids (challenged with S. suis ± Plg or Control) using the RNeasy Mini Kit (Qiagen). Three replicates were used for each condition and each replicate consisted of  three ChP organoids collected into a 15 mL tube. Organoids were dissociated using Accutase (STEMCELL, 07920) by pipetting up and down 10 times at 5 min intervals. Cells suspension was pelleted at 300 × g for 5 min, resuspended in 350 µL of Buffer RLT, and thoroughly disrupted by vortexing. 350 µL of 70% ethanol was added to tube and up to 700 µL of the sample was transferred to an RNeasy Mini spin column placed in a 2 mL collection tube.  Subsequently, genomic DNA was eliminated from RNA samples using DNA-freeTM DNA Removal Kit (Invitrogen AM 1906) following the manufacturer’s recommended protocol. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. First-strand cDNA was synthesized using random hexamer primers, followed by the second-strand cDNA synthesis using dTTP for non-directional library construction
description
adult
developmental stage
bacterial disease
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
choroid plexus (UBERON_0001886)
organism part
Homo sapiens
scientific_name
control
stimulus
Sample 1
title