2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
STANFORD UNIVERSITY
INSDC center name
public
INSDC status
ERS22949201
SRA accession
E-MTAB-14765:U87-MG uninfected
Submitter Id
ArrayExpress
broker name
U87-MG
cell_line
not collected
collection date
human
common name
Protocols: >500-fold coverage of mutagenized cells (Brunello library) was infected with EV-D68 IL (MOI 0.1). Resistant cell colonies were harvested. Uninfected starting populations of mutagenized cells were used as the unselected reference.  Total genomic DNA from both virus-resistant and uninfected cells was respectively extracted using QIAamp DNA Mini Kit (Qiagen). A first round of PCR was used to amplify the guide RNA sequences encoded in the gDNA, followed by a second round of PCR to add the barcodes/adapters for amplicon sequencing. 2% agarose gels and a QIAquick gel extraction kit were used to purify the amplicons.
description
brain glioblastoma
disease
lentiCas9-Blast; lentiGuide-Puro; lentiCas9-Blast
genotype
not collected
geographic location (country and/or sea)
none
infect
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
brain
organism part
Homo sapiens
scientific_name
U87-MG uninfected
title