2025-07-18
ENA first public
ERC000011
ENA-CHECKLIST
Shanghai Jiao Tong University
INSDC center name
public
INSDC status
ERS20302141
SRA accession
E-MTAB-14193:HepG2-shCtrl-rep2
Submitter Id
ArrayExpress
broker name
HepG2
cell_line
control cell
cell_type
not collected
collection date
human
common name
Protocols: HepG2 cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum. The cells were maintained at 37 °C in a 5% CO2 humidified atmosphere. When the cell density reached approximately 80%, the cells were collected. 5x10^6 HepG2 cells were used in shCtrl and YTHDC1 knockdown group. Total RNA was isolated by Trizol (Invitrogen) according to the manufacturer’s protocols. mRNA was purified by NEBNext Poly(A) mRNA Magnetic Isolation Beads (NEB, USA). The RNA libraries were constructed using the NEBNext Ultra Directional RNA Library PrepKit (NEB, USA).
description
hepatocellular carcinoma
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
HepG2-shCtrl-rep2
title