2025-07-19
ENA first public
ERC000033
ENA-CHECKLIST
Dutch COVID-19 response team
INSDC center name
public
INSDC status
ERS25269096
SRA accession
34217
Submitter Id
ERASMUS MC, UNIVERISTY MEDICAL CENTER
broker name
not provided
collecting institution
2025-04-03
collection date
Dutch COVID-19 response team
collector name
Human coronavirus 2019
common name
A SARS-CoV-2 specfic multiplex PCR for Nanopore sequencing was performed, similar to amplicon-based approaches as previously described. In short, primers for 86 overlapping amplicons spanning the entire genome were designed using primal. The amplicon length was set to 500bp with 75bp overlap between the different amplicons. The libraries were generated using the native barcode kits from Nanopore (EXP-NBD104 and EXP-NBD114 and SQK-LSK109) and sequenced on a R9.4 flow cell multiplexing up to 24 samples per sequence run. Raw data was demultiplexed, amplicon primers were trimmed and human data was removed by mapping against the human reference genome.
description
Netherlands
geographic location (country and/or sea)
EPI_ISL_20066321
gisaid accession id
Human
host common name
not provided
host health state
Homo sapiens
host scientific name
not provided
host sex
not provided
host subject id
hCov-19/Netherlands/NB-EMC-5447/03-04-2025
isolate
Severe acute respiratory syndrome coronavirus 2
organism
Severe acute respiratory syndrome coronavirus 2
scientific_name
SARS-CoV-2 sequence
title