2025-07-21
ENA first public
ERC000011
ENA-CHECKLIST
York Biomedical Research Institute
INSDC center name
public
INSDC status
ERS25171853
SRA accession
E-MTAB-15329:Input Control
Submitter Id
ArrayExpress
broker name
MCF-7
cell line
not collected
collection date
human
common name
Protocols: MCF7 cells (RRID:CVCL_0031) were cultured in DMEM (Gibco) with 10% FBS. Cells were seeded at 2 × 10⁶ per 15 cm dish and grown for 3 days. On day 4, media was replaced and cells were treated with 2 mM DMOG (or vehicle control). After 16 hours, cells were washed with PBS and crosslinked using 1% formaldehyde. Following immunoprecipitation, protein–DNA complexes were eluted, reverse crosslinked, and treated with RNase and proteinase K. DNA was purified using phenol-chloroform extraction and ethanol precipitation. ChIP DNA was used as input for library prep using the NEBNext Ultra II DNA Library Prep Kit, including end repair, adaptor ligation, size selection, and PCR amplification.
description
estrogen-receptor positive breast cancer
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
mammary gland
organism part
Homo sapiens
scientific_name
Input Control
title