2025-07-23
ENA first public
ERC000011
ENA-CHECKLIST
Institute for Regenerative Medicine & Biotherapy (IRMB) U1183 - INSERM - Universite Montpellier - CHU
INSDC center name
public
INSDC status
ERS18100591
SRA accession
E-MTAB-13763:CMML9
Submitter Id
not available
age
ArrayExpress
broker name
CD14 purified cells
cell_type
not collected
collection date
human
common name
Protocols: 10 samples from Bone marrow or peripheral blood separated on Fycoll-Hypaque. CD14+ monocytes were then sorted from PBMCs with magnetic beads and the AutoMacs system as previously described by Merlevede et al. (2016) Total RNA was isolated using mini RNeasy kit (Qiagen) according to the manufacturer instructions. The quantity and quality of extracted RNA was evaluated using the NanoDrop ND-1000 spectrophotometer and the Agilent 2100 BioAnalyzer.  Libraries have been prepared using the “TruSeq Stranded Total RNA Gold” Kit from Illumina.
description
adult
developmental stage
chronic myelomonocytic leukemia
disease
not collected
geographic location (country and/or sea)
9
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood component
organism part
Homo sapiens
scientific_name
male
sex
CMML9
title