2025-07-24
ENA first public
ERC000013
ENA-CHECKLIST
HIOH
INSDC center name
public
INSDC status
ERS24827774
SRA accession
PCRblank5_20250211_Rep2
Submitter Id
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT
adapters
natural
biological status
laboratory environment (ENVO:01001405)
broad-scale environmental context
2025-02-11
collection date
PCR blank consisting of Nuclease-free water, processed alongside feacal DNA extracts to monitor for contamination during the metabarcoding analyses. PCR performed on 2025-02-11
description
laboratory environment (ENVO:01001405)
environmental medium
Germany
geographic location (country and/or sea)
not collected
DD
geographic location (latitude)
not collected
DD
geographic location (longitude)
not applicable
geographic location (region and locality)
not applicable
host body habitat
not applicable
host body product
not applicable
host body site
not applicable
host common name
not applicable
host diet
not applicable
host disease status
not applicable
host life stage
not applicable
host scientific name
not applicable
host sex
not applicable
host subject id
2582415
host taxid
Tagsteady protocol with dual-indexed (P5 and P7) Illumina Y-adapters (https://doi.org/10.1111/1755-0998.13227)
library construction method
15.151
library reads sequenced
279
library size
laboratory environment (ENVO:01001405)
local environmental context
ACATTC : CAATCG
multiplex identifiers
Tagged conventional PCR (https://doi.org/10.1371/journal.pone.0000197 - modificactions indicated in PCR conditions and primers)
nucleic acid amplification
not applicable
nucleic acid extraction
blank sample
organism
95degC_10min, 35 cycles of 95degC_12s, 59degC_30s, 70degC_25s, and 72degC_7min
pcr conditions
16Smam1 5'-CGGTTGGGGTGACCTCGGA-3' and 16S mam2 5'-GCTGTTATCCCTAGGGTAACT-3'
pcr primers
Fire-footed rope squirrels (Funisciurus pyrropus) are a reservoir host of monkeypox virus (Orthopoxvirus monkeypox)
project name
other
sample capture status
not applicable
sample collection device
not applicable
sample collection method
P0.5Y
sample storage duration
4
°C
sample storage temperature
blank sample
scientific_name
not applicable
source material identifiers
16S mitochondrial DNA
target gene
130 bp fragment of 16S mitochondrial DNA
target subfragment
PCR blank (nuclease-free water), 2025-02-11
title