2025-07-24
ENA first public
ERC000013
ENA-CHECKLIST
HIOH
INSDC center name
public
INSDC status
ERS24827742
SRA accession
Metabarcoding_FecesBAK_CA_TNP_202212230_Rep3
Submitter Id
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT
adapters
wild
biological status
tropical rainforest biome (ENVO:01000228)
broad-scale environmental context
2022-12-30
collection date
Fecal sample collected from a sooty mangabey (Cercocebus atys) named BAK in Ta� National Park, C�te d'Ivoire, on 2022-12-30. Sample used for dietary analysis via 16S mitocondrial DNA metabarcoding.
description
forest soil (ENVO:00002261)
environmental medium
Cote d'Ivoire
geographic location (country and/or sea)
not collected
DD
geographic location (latitude)
not collected
DD
geographic location (longitude)
Cote d'Ivoire: Tai National Park
geographic location (region and locality)
gastrointestinal tract
host body habitat
stool
host body product
feces
host body site
Sooty mangabey
host common name
omnivore
host diet
Healthy
host disease status
adult
host life stage
Cercocebus atys atys
host scientific name
male
host sex
BAK
host subject id
1592215
host taxid
Tagsteady protocol with dual-indexed (P5 and P7) Illumina Y-adapters (https://doi.org/10.1111/1755-0998.13227)
library construction method
15.151
library reads sequenced
279
library size
forest floor (ENVO:01001582)
local environmental context
GTAATT : CACTAA
multiplex identifiers
Tagged conventional PCR (https://doi.org/10.1371/journal.pone.0000197 - modificactions indicated in PCR conditions and primers)
nucleic acid amplification
GeneMATRIX Stool DNA Purification Kit (Roboklon, Poland) - 40mg sample
nucleic acid extraction
feces metagenome
organism
95degC_10min, 35 cycles of 95degC_12s, 59degC_30s, 70degC_25s, and 72degC_7min
pcr conditions
16Smam1 5'-CGGTTGGGGTGACCTCGGA-3' and 16S mam2 5'-GCTGTTATCCCTAGGGTAACT-3'
pcr primers
Fire-footed rope squirrels (Funisciurus pyrropus) are a reservoir host of monkeypox virus (Orthopoxvirus monkeypox)
project name
active surveillance not initiated by an outbreak
sample capture status
plastic spatula and 2 mL cryotube
sample collection device
Faecal sample was collected from the ground right after defecation occurred with a plastic spatula and stored in a 2 mL cryotube
sample collection method
P2Y
sample storage duration
-80
°C
sample storage temperature
feces metagenome
scientific_name
BAK_feces_202212230
source material identifiers
16S mitochondrial DNA
target gene
130 bp fragment of 16S mitochondrial DNA
target subfragment
Fecal sample from sooty mangabey BAK, Ta� National Park, 2022-12-30, for 16S metabarcoding
title