2025-07-24
ENA first public
ERC000013
ENA-CHECKLIST
HIOH
INSDC center name
public
INSDC status
ERS24827778
SRA accession
PositiveControl_20250211_Rep3
Submitter Id
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT
adapters
breeder's line
biological status
laboratory environment (ENVO:01001405)
broad-scale environmental context
2025-02-11
collection date
domestic cattle
common name
Positive control consisting of german beef muscle previously bought in a german supermarket. PCR performed on 2025-02-11
description
laboratory environment (ENVO:01001405)
environmental medium
Germany
geographic location (country and/or sea)
not collected
DD
geographic location (latitude)
not collected
DD
geographic location (longitude)
not applicable
geographic location (region and locality)
skeletal muscle tissue
host body habitat
not applicable
host body product
skeletal muscle
host body site
Cattle
host common name
herbivore
host diet
Healthy
host disease status
not provided
host life stage
Bos taurus
host scientific name
not provided
host sex
not provided
host subject id
9913
host taxid
Tagsteady protocol with dual-indexed (P5 and P7) Illumina Y-adapters (https://doi.org/10.1111/1755-0998.13227)
library construction method
15.151
library reads sequenced
279
library size
laboratory environment (ENVO:01001405)
local environmental context
AGTCTC : CAGGAT
multiplex identifiers
Tagged conventional PCR (https://doi.org/10.1371/journal.pone.0000197 - modificactions indicated in PCR conditions and primers)
nucleic acid amplification
DNeasy Blood and Tissue kit (Qiagen, Germany) - 20mg sample
nucleic acid extraction
Bos taurus
organism
95degC_10min, 35 cycles of 95degC_12s, 59degC_30s, 70degC_25s, and 72degC_7min
pcr conditions
16Smam1 5'-CGGTTGGGGTGACCTCGGA-3' and 16S mam2 5'-GCTGTTATCCCTAGGGTAACT-3'
pcr primers
Fire-footed rope squirrels (Funisciurus pyrropus) are a reservoir host of monkeypox virus (Orthopoxvirus monkeypox)
project name
other
sample capture status
scalpel and tweezers
sample collection device
Positive control prepared using a small piece of raw beef muscle (Bos taurus) purchased from a supermarket in Germany
sample collection method
P0.5Y
sample storage duration
-20
°C
sample storage temperature
Bos taurus
scientific_name
not provided
source material identifiers
16S mitochondrial DNA
target gene
130 bp fragment of 16S mitochondrial DNA
target subfragment
Positive Control (beef muscle), 2025-02-11
title