2025-07-25
ENA first public
ERC000011
ENA-CHECKLIST
Istituto Italiano di Tecnologia - IIT
INSDC center name
public
INSDC status
ERS24548701
SRA accession
E-MTAB-15146:Sample 13
Submitter Id
ArrayExpress
broker name
CHO-K1
cell line
ovary cell
cell type
not collected
collection date
Chinese hamster
common name
Protocols: Transfections were carried out in 24-well plate for flow cytometry analysis.   H1299 cells were transfected with Lipofectamine® 3000 according to manufacturer’s instructions and 300 ng of total DNA in 24-well plates. CHO and HEK293 were transfected with PEI transfection reagent with 300 ng of total DNA in 24-well plates. All cells were plated approximately 24h before transfection at 70000 to 80000 cells per well in 24-wells plates. At the moment of transfection, cells were put in starvation conditions, meaning in poor media for the first 24h. DNA was diluted in Opti-MEM reduced serum media (Gibco), before being mixed and incubated for 25 minutes prior to addition to the cells. All drugs were added 4h after transfection.  Modified-RNAs (modRNA) were transfected using Lipofectamine® 3000 protocol under modified conditions in which no P3000 reagent was used. RNA was first diluted in Opti-MEM reduced serum media (Gibco) and it was then mixed with diluted Lipofectamine reagent as manufacturer’s instructions. The mix was incubated for 7 minutes prior to addition to the cells. The fast-forward protocol was used by seeding 120000 cells per well in 24-wells plates at the moment of transfection.  Libraries were prepared by Total RNAseq with ribo0 plus (Illumina)
description
adult
developmental stage
No disease related
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Cricetulus griseus
organism
Cricetulus griseus
organism
ovary
organism part
Cricetulus griseus
scientific_name
Sample 13
title