2025-07-25
ENA first public
ERC000011
ENA-CHECKLIST
Department of Surgery, Yonsei University College of Medicine
INSDC center name
public
INSDC status
ERS25114672
SRA accession
E-MTAB-15233:363
Submitter Id
63
year
age
ArrayExpress
broker name
Cluster 2 High
clinical history
not collected
collection date
human
common name
Protocols: Patients with papillary thyroid carcinoma (PTC) underwent thyroidectomy at Yonsei Cancer Center or Yongin Severance Hospital. Resected tissues were snap-frozen in liquid nitrogen immediately and stored at –80 °C. Cytology was classified as Bethesda V/VI, and risk was stratified using ATA 2015 guidelines. Total RNA was extracted using TRIzol® reagent (Invitrogen) per manufacturer's instructions. RNA concentration was measured using Quant-IT RiboGreen assay and quality evaluated using Agilent TapeStation. Only RNA with RIN ≥ 7.0 was used. RNA libraries were prepared using the Illumina TruSeq Stranded mRNA Sample Prep Kit. mRNA was isolated with poly-T magnetic beads, fragmented, and converted to cDNA, followed by end repair, adapter ligation, and PCR enrichment.
description
adult
developmental stage
thyroid cancer
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
thyroid gland
organism part
Homo sapiens
scientific_name
female
sex
363
title