2025-07-26
ENA first public
ERC000011
ENA-CHECKLIST
SJTU
INSDC center name
public
INSDC status
ERS20599300
SRA accession
E-MTAB-14256:WT FGSCs-Input
Submitter Id
ArrayExpress
broker name
FGSC
cell_line
germ line stem cell
cell_type
not collected
collection date
house mouse
common name
Protocols: FGSCs were cultured on STO feeder cells using Minimum Essential Medium-ɑ, supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 1 mM non-essential amino acid , 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, 10 ng/mL mouse epidermal growth factor , 10 ng/mL mouse leukemia inhibitory factor, 40 ng/mL mouse glial cell line-derived neurotrophic factor, 10 ng/mL human basic fibroblast growth factor, and 1% penicillin-streptomycin. The cells were maintained at 37 °C in a 5% CO2 humidified atmosphere. When the cell density reached approximately 80%, the cells were collected. Total RNA was isolated by Trizol (Invitrogen) according to the manufacturer’s protocols. RNA was purified by NEBNext® rRNA Depletion Kit (NEB, USA). Fragmented and immunoprecipitated with anti-m6A antibody (Synaptic Systems, 202003). The RNA libraries were constructed using the NEBNext Ultra Directional RNA Library PrepKit (NEB, USA).
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
ovary
organism part
Mus musculus
scientific_name
WT FGSCs-Input
title