2025-07-26
ENA first public
ERC000011
ENA-CHECKLIST
SJTU
INSDC center name
public
INSDC status
ERS20599304
SRA accession
E-MTAB-14257:WT FGSCs，RNA-seq，rep2
Submitter Id
ArrayExpress
broker name
FGSC
cell_line
germ line stem cell
cell_type
not collected
collection date
house mouse
common name
Protocols: FGSCs were cultured on STO feeder cells using Minimum Essential Medium-ɑ, supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 1 mM non-essential amino acid , 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, 10 ng/mL mouse epidermal growth factor , 10 ng/mL mouse leukemia inhibitory factor, 40 ng/mL mouse glial cell line-derived neurotrophic factor, 10 ng/mL human basic fibroblast growth factor, and 1% penicillin-streptomycin. The cells were maintained at 37 °C in a 5% CO2 humidified atmosphere. When the cell density reached approximately 80%, the cells were collected. Total RNA was isolated by Trizol (Invitrogen) according to the manufacturer’s protocols. mRNA was purified by NEBNext Poly(A) mRNA Magnetic Isolation Beads (NEB, USA). The RNA libraries were constructed using the NEBNext Ultra Directional RNA Library PrepKit (NEB, USA).
description
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
ovary
organism part
rep2
replicate
Mus musculus
scientific_name
WT FGSCs，RNA-seq，rep2
title