barrnap (version 0.9)
16S recovery software
No
16s recovered
No
23s recovered
No
5s recovered
2025-07-29
ENA first public
ERC000047
ENA-CHECKLIST
31.3
%
GC content
CAU
INSDC center name
public
INSDC status
Hisat2 (version 2.1.0) with jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1)
MAG coverage software
ERS25318149
SRA accession
ena-SAMPLE-TAB-24-07-2025-15:39:17:964-14846
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
OPERA-MS 0.9 using SPAdes 3.13.0
assembly software
coverage depth and k-mer binning. Scaffolds were filtered for min. length 1000bp and min. coverage 37. Quality-controlled metagenomic reads were mapped back to the filtered scaffolds with Hisat2 (version 2.1.0); the insert size was 0–1000 bp in non-deterministic, “fr” stranded mode with end-to-end alignment. Non-unique mappings and unaligned reads were discarded. The scaffold coverage depth was determined with the jgi_summarize_bam_contig_depths script from MetaBAT (version 2.12.1). Scaffolds were broken into 10 kbp chunks for CONCOCT.
binning parameters
MetaBAT (version 2.12.1), Autometa (version 2.0a0), CONCOCT (version 1.1.0), MaxBin (version 2.2.4), DASTool (version 1.1.2)
binning software
host associated gut microbiome
broad-scale environmental context
2020-08-05
collection date
94.4
%
completeness score
CheckM (version 1.1.2)
completeness software
1.7
%
contamination score
MAG_CA_Bacilli_03: Metagenomic assembled genome (MAG) from colon contents of n=135, untreated, wild-type, male C57BL/6J/Ukj mice, aged 2-30 months. Full cohort assembly of all metagenomic samples assembled together from short and long read sequencing with OPERA-MS 0.9 using SPAdes 3.13.0. Coverage and k-mer binning with MetaBAT (version 2.12.1), Autometa (version 2.0a0), CONCOCT (version 1.1.0), and MaxBin (version 2.2.4) produced bins which were refined into 383 MAGs via DASTool, of which 249 MAGs were of medium to high quality.
description
host associated gut microbiome
environmental medium
Germany
geographic location (country and/or sea)
50.9
DD
geographic location (latitude)
11.6
DD
geographic location (longitude)
mouse colon contents
isolation_source
host associated gut microbiome
local environmental context
mouse gut metagenome
metagenomic source
18
number of standard tRNAs extracted
uncultured Bacilli bacterium
organism
Cognition aging (CA); Aging-related cognitive decline linked with systemic inflammation and alterations of tissue-maintenance
project name
ERS25309092,ERS25309091,ERS25309090,ERS25309089,ERS25309088,ERS25309087,ERS25309086,ERS25309085,ERS25309084,ERS25309083,ERS25309082,ERS25309081,ERS25309080,ERS25309079,ERS25309078,ERS25309077,ERS25309076,ERS25309075,ERS25309074,ERS25309073,ERS25309072,ERS25309071,ERS25309070,ERS25309069,ERS25309068,ERS25309067,ERS25309066,ERS25309065,ERS25309064,ERS25309063,ERS25309062,ERS25309061,ERS25309060,ERS25309059,ERS25309058,ERS25309057,ERS25309056,ERS25309055,ERS25309054,ERS25309053,ERS25309052,ERS25309051,ERS25309050,ERS25309049,ERS25309048,ERS25309047,ERS25309046,ERS25309045,ERS25309044,ERS25309043,ERS25309042,ERS25309041,ERS25309040,ERS25309039,ERS25309038,ERS25309037,ERS25309036,ERS25309035,ERS25309034,ERS25309033,ERS25309032,ERS25309031,ERS25309030,ERS25309029,ERS25309028,ERS25309027,ERS25309026,ERS25309025,ERS25309024,ERS25309023,ERS25309022,ERS25309021,ERS25309020,ERS25309019,ERS25309018,ERS25309017,ERS25309016,ERS25309015,ERS25309014,ERS25309013,ERS25309012,ERS25309011,ERS25309010,ERS18444878,ERS18444879,ERS18444880,ERS18444881,ERS18444882,ERS18444883,ERS18444884,ERS18444885,ERS18444886,ERS18444887,ERS18444888,ERS18444889,ERS18444890,ERS18444891,ERS18444892,ERS18444893,ERS18444894,ERS18444895,ERS18444896,ERS18444897,ERS18444898,ERS18444899,ERS18444900,ERS18444901,ERS18444902,ERS18444903,ERS18444904,ERS18444905,ERS18444906,ERS18444907,ERS18444908,ERS18444909,ERS18444910,ERS18444911,ERS18444912,ERS18444913,ERS18444914,ERS18444915,ERS18444916,ERS18444917,ERS18444918,ERS18444919,ERS18444920,ERS18444921,ERS18444922,ERS18444923,ERS18444924,ERS18444925,ERS18444926,ERS18444927,ERS18444928,ERS18444929,ERS18590020,ERS18590021,ERS18590022,ERS18590023,ERS18590024,ERS18590025,ERS18590026,ERS18590027
sample derived from
uncultured Bacilli bacterium
scientific_name
tRNAscan-SE (version 2.0.12)
tRNA extraction software
GTDB-Tk, r214
taxonomic classification
multi-marker approach
taxonomic identity marker
CA_Bacilli_03
title