2025-07-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114307770
External Id
Dementia Research Institute at KCL, London, UK The Francis Crick Institute, London, UK National Institute of Chemistry, Ljubljana, Slovenia
INSDC center name
2023-08-29T11:07:22Z
INSDC last update
public
INSDC status
ERS16296772
SRA accession
E-MTAB-13304:h12_N_2
Submitter Id
ArrayExpress
broker name
HeLa
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: Samples were collected by trypsinising, spinning down, washing in PBS, spinning down and snap freezing on dry ice. Cells were grown in DMEM+Glutamax+10% FBS and seeded such that they achieved 70% confluency on the morning of the experiment, with each sample being derived from 2 wells of a 6 well plate. Pre-warmed doxycycline was added to cells either for either 0, 4, 8 or 12 hours prior to collection. RNA extractions were performed from cell pellets using the Maxwell RSC simplyRNA kit (Promega) using a Maxwell RSC Instrument (Promega). RNA was fragmented using magnesium ions and heat, purified, and reverse transcribed using an oligo-dT primer with an Illumina P7 sequence and UMIs. A template switching oligo with the P5 sequence was included in the reaction. The library was then amplified with indexed Illumina i5/i7 primers.
description
adult
developmental stage
cervical cancer
disease
nucleus
fraction
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
cervix epithelium
organism part
Homo sapiens
scientific_name
h12_N_2
title