2025-07-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114327418
External Id
Dementia Research Institute at KCL, London, UK The Francis Crick Institute, London, UK National Institute of Chemistry, Ljubljana, Slovenia
INSDC center name
2023-09-04T15:31:10Z
INSDC last update
public
INSDC status
ERS16316528
SRA accession
E-MTAB-13330:rna_sequencing
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Samples were derived from plasmid DNA, or from whole cells transfected for 16 hours with the plasmid pool. In the case of the transfected cells, cells were grown in DMEM+Glutamax+10% FBS and seeded such that they achieved 70% confluency on the morning of the experiment, with each sample being derived from 2 wells of a 6 well plate. In the case of the transfected cells, cells were transfected with the plasmid pool using Lipofectamine 3000 according to manufacturer instructions. In the case of the transfected cells, RNA extractions were performed from cell pellets using the Maxwell RSC simplyRNA kit (Promega) using a Maxwell RSC Instrument (Promega). Targeted PCR of the whole transcript, followed by Nanopore adapter ligation. In the case of the RNA sequencing, we performed oligo-dT based RT using Superscript IV
description
RNA barcoded pool of transcripts
genotype
not collected
geographic location (country and/or sea)
grown in HeLa cells
growth condition
not applicable
isolate
synthetic construct
organism
synthetic construct
organism
whole organism
organism part
synthetic construct
scientific_name
rna_sequencing
title