2025-07-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114327511
External Id
Dementia Research Institute at KCL, London, UK The Francis Crick Institute, London, UK National Institute of Chemistry, Ljubljana, Slovenia
INSDC center name
2023-09-04T16:34:28Z
INSDC last update
public
INSDC status
ERS16316621
SRA accession
E-MTAB-13329:clk-in-t3_6hr
Submitter Id
ArrayExpress
broker name
HeLa
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: Samples were collected by trypsinising, spinning down, washing in PBS, spinning down and snap freezing on dry ice. Cells were grown in DMEM+Glutamax+10% FBS and seeded such that they achieved 70% confluency on the morning of the experiment, with each sample being derived from 2 wells of a 6 well plate. Cells were either transfected, dox-induced, and sometimes drug treated for the period of time described in the accession description and sample names. RNA extractions were performed from cell pellets using the Maxwell RSC simplyRNA kit (Promega) using a Maxwell RSC Instrument (Promega). Superscript IV RT using a barcoded RT primer specific to the reporter transcript. Specific amplification of the barcoded sequences using nested PCR. Final library amplification using indexed i5/i7 Illumina primers.
description
adult
developmental stage
cervical cancer
disease
barcoded transcript pool
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
cervix epithelium
organism part
Homo sapiens
scientific_name
clk-in-t3_6hr
title