2025-07-31
ENA first public
ERC000011
ENA-CHECKLIST
Department of Biology, University of Fribourg.
INSDC center name
public
INSDC status
ERS21137440
SRA accession
E-MTAB-14523:N204_wildtype
Submitter Id
50 hours post-L1 stage
hour
age
ArrayExpress
broker name
not collected
collection date
Protocols: At least 1000 animals from wild-type and lsy-2(ot64) strains were synchronized and collected as young adult hermaphrodites 50 hours post-larval hatching after hypochlorite treatment, at 20°C (Stiernagle, T. 2006). Total RNA was extracted with TRIzol Reagent (Invitrogen; Carlsbad CA, USA) and RNA was purified using the PureLink RNA Mini Kit (Invitrogen; Carlsbad IA, USA) according to the manufacturer's instructions cDNA libraries were built using the TruSeq stranded mRNA library preparation kit (Illumina Inc.; San Diego CA, USA) by the Next Generation Sequencing (NGS) Platform in Bern (https://www.ngs.unibe.ch/)
description
newly molted young adult hermaphrodite
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Caenorhabditis elegans
organism
Caenorhabditis elegans
organism
whole organism
organism part
Caenorhabditis elegans
scientific_name
hermaphrodite
sex
N2
strain
N204_wildtype
title