2025-07-31
ENA first public
ERC000011
ENA-CHECKLIST
Charité-Universitätsmedizin Berlin Department of Endocrinology and Metabolism
INSDC center name
public
INSDC status
ERS23860073
SRA accession
E-MTAB-14973:GEX-BLK_0124
Submitter Id
ArrayExpress
broker name
BIHi001-B
cell line
hiPSC-derived Hepatocyte-Like Cells
cell type
not collected
collection date
human
common name
Protocols: On day 28 of differentiation, the cell culture medium was discarded, and cells were transferred and stored at -80 °C. On the day of RNA extraction, plates were thawed on ice, lysis buffer was added to the culture wells, and the surface was scraped to maximize lysate yield. RNA from samples was extracted with Aurum™ Total RNA Mini Kit (Bio-Rad) according to manufacturer's instructions. Bulk RNA-seq libraries were generated using Poly(A) mRNA selection and prepared with the NEBNext Ultra II RNA Library Prep Kit for Illumina, following the manufacturer’s instructions.
description
neonate
developmental stage
No disease was diagnosed.
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
no T3
growth condition
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
dermal fibroblast
organism part
Homo sapiens
scientific_name
GEX-BLK_0124
title