2025-07-31
ENA first public
ERC000011
ENA-CHECKLIST
CAMS China Oxford Institute (CAMS-COI)
INSDC center name
public
INSDC status
ERS23797886
SRA accession
E-MTAB-14933_2:Sample 1584
Submitter Id
69
year
age
ArrayExpress
broker name
CD4-positive, alpha-beta T cell
cell_type
not collected
collection date
human
common name
Protocols: Patients were recruited from the John Radcliffe Hospital in Oxford, UK, between March 2020 and September 2021 by identification of patients hospitalized during the SARS-CoV-2 pandemic and recruited into the Sepsis Immunomics study. All patients were sampled at least 28 days after symptom onset during the primary infection, while 10 patients were further sampled around 3-4 years after initial infection. Single cells were directly sorted into 96-well PCR plates (Thermo Fisher Scientific) containing cell lysis buffer and stored at −80°C for further SmartSeq2 analysis. ScRNA-seq with ex vivo tetramer+ cells was performed using SmartSeq2 analysis as described previously (Picelli et al (2014)). Reverse-transcription (RT) and PCR amplification were performed with the exception of using ISPCR primer with biotin tagged at the 5′ end and increasing the number of cycles to 25. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit
description
adult
developmental stage
COVID-19
disease
S751-765
epitope
not collected
geographic location (country and/or sea)
C_COV19_047
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood component
organism part
Homo sapiens
scientific_name
male
sex
Sample 1584
title