2025-07-31
ENA first public
ERC000011
ENA-CHECKLIST
Mautner BHF Career Development Fellow Discovery and Translation Science Department Leeds Institute of Cardiovascular and Metabolic Medicine
INSDC center name
public
INSDC status
ERS21051350
SRA accession
E-MTAB-14458:Sample 1
Submitter Id
ArrayExpress
broker name
endothelial cell derived cell line
cell_line
endothelial cell
cell_type
not collected
collection date
house mouse
common name
Protocols: We generated mice with endothelial-restricted over-expression of human wildtype IGF-1R (hIGFREO/ApoE-/-) or a signaling defective K1003R mutant human IGF-1R (mIGFREO/ApoE-/-) Total RNA was prepared from tissues using TriZol reagent (Sigma). RNA sequencing was performed by sequencing facilities at the University of Leeds. After the QC procedures, mRNA from eukaryotic organisms is enriched using oligo(dT) beads. First, the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.
description
fetal
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
umbilical vein
organism part
Mus musculus
scientific_name
Sample 1
title