2025-07-31
ENA first public
ERC000011
ENA-CHECKLIST
Research Institute for Farm Animal Biology (FBN), Physiological Genomics
INSDC center name
public
INSDC status
ERS21050031
SRA accession
E-MTAB-14452:Tal_11
Submitter Id
170
day
age
ArrayExpress
broker name
not collected
collection date
pig
common name
Protocols: Animal handling and compassionate euthanasia were performed in compliance with pertinent ethical laws, standards, and regulations. The study included 8 female German Landrace pigs, with an average age of 170 ± 14 days and weight of 105 ± 8 kg. The adrenal gland and brain tissues, including the amygdala, hippocampus, hypothalamus, pituitary gland, and thalamus were swiftly removed, flash-frozen in liquid nitrogen, and preserved at − 80 °C for subsequent analyses.  Tissue sample was finely grounded in liquid nitrogen into powder. Total RNA was purified using the RNeasy Mini Kit (Qiagen, Germany) and treated with DNase I (Roche Diagnostics, Mannheim, Germany) to remove trace DNA contamination. The RNA quantity and purity were assessed using a NanoDrop ND-1000 spectrophotometer (Peqlab) and the RNA integrity was determined using the RNA 6000 Nano Kit for 2100 Bioanalyzer System (Agilent Technologies). DNA libraries were generated using the Illumina Stranded mRNA preparation kit (Illumina, San Diego, CA, USA).
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Sus scrofa
organism
Sus scrofa
organism
thalamus
organism part
Sus scrofa
scientific_name
female
sex
German Landrace
strain
Tal_11
title