2025-07-31
ENA first public
ERC000011
ENA-CHECKLIST
EMBL
INSDC center name
public
INSDC status
ERS25339141
SRA accession
ena-upload-cli
SUBMISSION_TOOL
0.9.0
SUBMISSION_TOOL_VERSION
c30b8372-c39c-4926-aadc-ee766cb9522a
Submitter Id
10-12 weeks
age
not provided
collection date
house mouse
common name
In vitro fertilizations followed a modified protocol (Guan et al. 2014) to increase throughput and synchronize development. Sperm was collected from the cauda epididymis and vasa deferentia of treated males, incubated in HTF capacitation media, and allowed to swim up. Cumulus-oocyte complexes were collected from superovulated females treated with PMSG and hCG in KSOM media, then inseminated with 0.2 million sperm in KSOM + GSH media for four hours in low-oxygen incubator conditions. Zygotes were then washed and cultured to the blastocyst stage under low oxygen (5% CO2, 5% O2 for FVB and B6J backgrounds) or standard oxygen (5% CO2, 20% O2 for B6N background) conditions.
description
Blastocyst
dev_stage
wild type
genotype
not applicable
geographic location (country and/or sea)
birth
initial time point
Mus musculus
organism
Blastocyst
organism part
Mus musculus
scientific_name
unknown sex
sex
fvb
strain
BlastoFVBxxY3xx1xx22
subject_id
BlastoFVBxxY3xx1xx22
title
control
treatment