2025-07-31
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114526822
External Id
Leeds Institute of Cardiovascular and Metabolic Medicine
INSDC center name
2023-10-23T20:46:14Z
INSDC last update
public
INSDC status
ERS16508158
SRA accession
E-MTAB-13481:Sample 7
Submitter Id
18
month
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Mice aged 3 or 18 months old underwent terminal isoflurane anesthesia followed by exsanguination. The brain (including cerebellum and brain stem) was harvested and hemisected longitudinally; the right side was mechanically pulverized (Qiagen TissueLyser II). The New England Biolabs Monarch Total RNA Miniprep Kit was used to isolate RNA and perform DNAse I treatment. RNA-seq was performed by the University of Leeds Next Generation Sequencing core facility (Illumina NextSeq 2000) to acquire 150 base pair single-end reads.
description
adult
developmental stage
IRKO
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
brain
organism part
Mus musculus
scientific_name
male
sex
C57BL/6J (664)
strain
Sample 7
title