2025-07-31
ENA first public
ERC000011
ENA-CHECKLIST
BAH-GU
INSDC center name
public
INSDC status
ERS25329576
SRA accession
E-MTAB-15349:INC0464
Submitter Id
50+
year
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: INCISE FFPE tissue full sections were cut to be 4μm thick and mounted on glass slides. To generate lysates, the full FFPE tissue was scraped off and placed in 1x FFPE Lysis Buffer (BioSpyder), and deparaffinised. Further processing included incubation in FFPE Protease (BioSpyder), followed by mechanical homogenization by trituration.  Detector oligo hybridization, digestion, and ligation, and subsequent PCR amplification was performed using a unique primer set for each sample, introducing a sample-specific barcode and Illumina adaptors according to manufacturer’s instructions.  Barcoded PCR products was added to a flip-spin reservoir and purified using the Macherey-Nagel NucleoSpin Gel and PCR Cleanup Kit (Cat. 740609.50, Macherey-Nagel) and quantified by Qubit using the dsDNA kit (Cat. Q32851, ThermoFisher Scientific), to generate a single library.
description
adult
developmental stage
colorectal adenoma
disease
not collected
geographic location (country and/or sea)
INC0464
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
colorectum
organism part
Homo sapiens
scientific_name
male
sex
Non-SOX9 Mutant
sox9 mutational status (group)
INC0464
title